Human Gene SHFL (ENST00000591813.5) from GENCODE V44
  Description: Homo sapiens shiftless antiviral inhibitor of ribosomal frameshifting (SHFL), transcript variant 2, mRNA. (from RefSeq NM_001308277)
Gencode Transcript: ENST00000591813.5
Gencode Gene: ENSG00000130813.18
Transcript (Including UTRs)
   Position: hg38 chr19:10,086,340-10,092,927 Size: 6,588 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr19:10,086,428-10,092,302 Size: 5,875 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:10,086,340-10,092,927)mRNA (may differ from genome)Protein (255 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
MalacardsMGIneXtProtOMIMPubMedUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CS066_HUMAN
DESCRIPTION: RecName: Full=UPF0515 protein C19orf66;
SIMILARITY: Belongs to the UPF0515 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 41.51 RPKM in Liver
Total median expression: 549.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.7088-0.440 Picture PostScript Text
3' UTR -229.80625-0.368 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026795 - UPF0515

ModBase Predicted Comparative 3D Structure on Q9NUL5
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding

Biological Process:
GO:0034340 response to type I interferon
GO:0034341 response to interferon-gamma
GO:0034342 response to type III interferon
GO:0045071 negative regulation of viral genome replication
GO:0051607 defense response to virus

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC026180 - Homo sapiens chromosome 19 open reading frame 66, mRNA (cDNA clone MGC:33008 IMAGE:4826265), complete cds.
AK002148 - Homo sapiens cDNA FLJ11286 fis, clone PLACE1009581.
BC010847 - Homo sapiens chromosome 19 open reading frame 66, mRNA (cDNA clone MGC:8786 IMAGE:3896535), complete cds.
BC035817 - Homo sapiens chromosome 19 open reading frame 66, mRNA (cDNA clone MGC:46073 IMAGE:5554801), complete cds.
AL137694 - Homo sapiens mRNA; cDNA DKFZp761N2124 (from clone DKFZp761N2124).
AK096142 - Homo sapiens cDNA FLJ38823 fis, clone LIVER2008751.
AK098062 - Homo sapiens cDNA FLJ40743 fis, clone TOVAR2000575.
JD046074 - Sequence 27098 from Patent EP1572962.
CU690214 - Synthetic construct Homo sapiens gateway clone IMAGE:100023284 5' read FLJ11286 mRNA.
KJ904804 - Synthetic construct Homo sapiens clone ccsbBroadEn_14198 C19orf66 gene, encodes complete protein.
AK001098 - Homo sapiens cDNA FLJ10236 fis, clone HEMBB1000391.
JD458277 - Sequence 439301 from Patent EP1572962.
KJ902811 - Synthetic construct Homo sapiens clone ccsbBroadEn_12205 C19orf66 gene, encodes complete protein.
JD544810 - Sequence 525834 from Patent EP1572962.
JD122947 - Sequence 103971 from Patent EP1572962.
JD171381 - Sequence 152405 from Patent EP1572962.
JD115458 - Sequence 96482 from Patent EP1572962.
JD231834 - Sequence 212858 from Patent EP1572962.
JD546336 - Sequence 527360 from Patent EP1572962.
JD051131 - Sequence 32155 from Patent EP1572962.
JD541395 - Sequence 522419 from Patent EP1572962.
JD038563 - Sequence 19587 from Patent EP1572962.
JD411668 - Sequence 392692 from Patent EP1572962.
JD252852 - Sequence 233876 from Patent EP1572962.
JD429811 - Sequence 410835 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8MQT9, C19orf66, CS066_HUMAN, ENST00000591813.1, ENST00000591813.2, ENST00000591813.3, ENST00000591813.4, NM_001308277, Q4G188, Q8IYH6, Q8N8V1, Q9NUL5, uc002mmv.1, uc002mmv.2, uc002mmv.3, uc002mmv.4, uc002mmv.5
UCSC ID: ENST00000591813.5
RefSeq Accession: NM_001308277
Protein: Q9NUL5 (aka CS066_HUMAN)
CCDS: CCDS77231.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.