Human Gene TMEM214 (ENST00000238788.14) from GENCODE V44
  Description: Homo sapiens transmembrane protein 214 (TMEM214), transcript variant 1, mRNA. (from RefSeq NM_017727)
Gencode Transcript: ENST00000238788.14
Gencode Gene: ENSG00000119777.20
Transcript (Including UTRs)
   Position: hg38 chr2:27,032,965-27,041,694 Size: 8,730 Total Exon Count: 17 Strand: +
Coding Region
   Position: hg38 chr2:27,033,016-27,040,837 Size: 7,822 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:27,032,965-27,041,694)mRNA (may differ from genome)Protein (689 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TM214_HUMAN
DESCRIPTION: RecName: Full=Transmembrane protein 214;
INTERACTION: O15116:LSM1; NbExp=1; IntAct=EBI-372318, EBI-347619;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the TMEM214 family.
SEQUENCE CAUTION: Sequence=AAL55739.1; Type=Erroneous initiation; Sequence=BAA91038.1; Type=Erroneous initiation; Sequence=BAC11331.1; Type=Frameshift; Positions=94; Sequence=CAH10591.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 74.08 RPKM in Pituitary
Total median expression: 1569.37 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.0051-0.314 Picture PostScript Text
3' UTR -257.50857-0.300 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019308 - DUF2359_TMEM214

Pfam Domains:
PF10151 - TMEM214, C-terminal, caspase 4 activator

ModBase Predicted Comparative 3D Structure on Q6NUQ4
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsembl  
Protein SequenceProtein SequenceProtein SequenceProtein Sequence  
AlignmentAlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0006915 apoptotic process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005881 cytoplasmic microtubule
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC068479 - Homo sapiens transmembrane protein 214, mRNA (cDNA clone MGC:87131 IMAGE:4814193), complete cds.
AK297150 - Homo sapiens cDNA FLJ57745 complete cds.
AK296575 - Homo sapiens cDNA FLJ58194 complete cds.
AK097308 - Homo sapiens cDNA FLJ39989 fis, clone STOMA2000678.
AK074981 - Homo sapiens cDNA FLJ90500 fis, clone NT2RP3004025.
CU686645 - Synthetic construct Homo sapiens gateway clone IMAGE:100022842 5' read FLJ20254 mRNA.
KJ899031 - Synthetic construct Homo sapiens clone ccsbBroadEn_08425 TMEM214 gene, encodes complete protein.
AL833725 - Homo sapiens mRNA; cDNA DKFZp666E065 (from clone DKFZp666E065).
BC002467 - Homo sapiens transmembrane protein 214, mRNA (cDNA clone IMAGE:3347870), partial cds.
JD372455 - Sequence 353479 from Patent EP1572962.
JD169932 - Sequence 150956 from Patent EP1572962.
JD390839 - Sequence 371863 from Patent EP1572962.
JD134660 - Sequence 115684 from Patent EP1572962.
JD190135 - Sequence 171159 from Patent EP1572962.
JD500789 - Sequence 481813 from Patent EP1572962.
AK315359 - Homo sapiens cDNA, FLJ96404.
JD159150 - Sequence 140174 from Patent EP1572962.
JD197451 - Sequence 178475 from Patent EP1572962.
JD566806 - Sequence 547830 from Patent EP1572962.
JD361175 - Sequence 342199 from Patent EP1572962.
JD424746 - Sequence 405770 from Patent EP1572962.
AK000261 - Homo sapiens cDNA FLJ20254 fis, clone COLF6926.
AL834157 - Homo sapiens mRNA; cDNA DKFZp761L1314 (from clone DKFZp761L1314).
AF289555 - Homo sapiens clone pp446 unknown mRNA.
AK097001 - Homo sapiens cDNA FLJ39682 fis, clone SMINT2010200.
JD555854 - Sequence 536878 from Patent EP1572962.
JD536982 - Sequence 518006 from Patent EP1572962.
JD181425 - Sequence 162449 from Patent EP1572962.
JD251565 - Sequence 232589 from Patent EP1572962.
JD470120 - Sequence 451144 from Patent EP1572962.
JD209966 - Sequence 190990 from Patent EP1572962.
JD370432 - Sequence 351456 from Patent EP1572962.
JD276294 - Sequence 257318 from Patent EP1572962.
JD123694 - Sequence 104718 from Patent EP1572962.
JD195323 - Sequence 176347 from Patent EP1572962.
JD165113 - Sequence 146137 from Patent EP1572962.
JD382392 - Sequence 363416 from Patent EP1572962.
JD165642 - Sequence 146666 from Patent EP1572962.
JD165643 - Sequence 146667 from Patent EP1572962.
JD543885 - Sequence 524909 from Patent EP1572962.
JD305325 - Sequence 286349 from Patent EP1572962.
JD335894 - Sequence 316918 from Patent EP1572962.
JD280812 - Sequence 261836 from Patent EP1572962.
JD288770 - Sequence 269794 from Patent EP1572962.
JD165639 - Sequence 146663 from Patent EP1572962.
JD201106 - Sequence 182130 from Patent EP1572962.
JD164732 - Sequence 145756 from Patent EP1572962.
JD103843 - Sequence 84867 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A6NNF2, B3KUI9, B5MCD8, D6W547, ENST00000238788.1, ENST00000238788.10, ENST00000238788.11, ENST00000238788.12, ENST00000238788.13, ENST00000238788.2, ENST00000238788.3, ENST00000238788.4, ENST00000238788.5, ENST00000238788.6, ENST00000238788.7, ENST00000238788.8, ENST00000238788.9, NM_017727, PP446, Q53SW1, Q69YH4, Q6NUQ4, Q8NC45, Q8WZ37, Q9NXH2, TM214_HUMAN, uc002ria.1, uc002ria.2, uc002ria.3, uc002ria.4, uc002ria.5, uc002ria.6
UCSC ID: ENST00000238788.14
RefSeq Accession: NM_017727
Protein: Q6NUQ4 (aka TM214_HUMAN)
CCDS: CCDS42664.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.