Human Gene CHST2 (ENST00000309575.5) from GENCODE V44
  Description: Homo sapiens carbohydrate sulfotransferase 2 (CHST2), mRNA. (from RefSeq NM_004267)
RefSeq Summary (NM_004267): This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gencode Transcript: ENST00000309575.5
Gencode Gene: ENSG00000175040.6
Transcript (Including UTRs)
   Position: hg38 chr3:143,119,771-143,124,014 Size: 4,244 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg38 chr3:143,120,817-143,122,409 Size: 1,593 Coding Exon Count: 1 

Page IndexSequence and LinksPrimersCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureOther SpeciesmRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:143,119,771-143,124,014)mRNA (may differ from genome)Protein (530 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGImyGene2
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 27.96 RPKM in Brain - Spinal cord (cervical c-1)
Total median expression: 358.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -503.00944-0.533 Picture PostScript Text
3' UTR -442.001605-0.275 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Descriptions from all associated GenBank mRNAs
  LF208581 - JP 2014500723-A/16084: Polycomb-Associated Non-Coding RNAs.
MA444158 - JP 2018138019-A/16084: Polycomb-Associated Non-Coding RNAs.
AB021124 - Homo sapiens mRNA for N-acetylglucosamine 6-O-sulfotransferase, complete cds.
BC042160 - Homo sapiens cDNA clone IMAGE:4871530, containing frame-shift errors.
AK023268 - Homo sapiens cDNA FLJ13206 fis, clone NT2RP3004670, highly similar to Carbohydrate sulfotransferase 2 (EC 2.8.2.-).
BC068604 - Homo sapiens carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, mRNA (cDNA clone IMAGE:5287173).
BC036930 - Homo sapiens carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, mRNA (cDNA clone IMAGE:5244181).
LF212301 - JP 2014500723-A/19804: Polycomb-Associated Non-Coding RNAs.
MA447878 - JP 2018138019-A/19804: Polycomb-Associated Non-Coding RNAs.
BC017499 - Homo sapiens carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, mRNA (cDNA clone IMAGE:4562846).
AB014679 - Homo sapiens GN6ST mRNA for N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST), complete cds.
AB014680 - Homo sapiens GN6ST mRNA for long form of N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST), complete cds.
JD278679 - Sequence 259703 from Patent EP1572962.
JD394391 - Sequence 375415 from Patent EP1572962.
AF083066 - Homo sapiens carbohydrate sulfotransferase 2 (CHST2) mRNA, complete cds.
BC105010 - Homo sapiens carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, mRNA (cDNA clone MGC:132670 IMAGE:8144013), complete cds.
BC105012 - Homo sapiens carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, mRNA (cDNA clone MGC:132672 IMAGE:8144015), complete cds.
EU668328 - Homo sapiens epididymis secretory protein Li 75 (HEL-S-75) mRNA, complete cds.
AB590672 - Synthetic construct DNA, clone: pFN21AE1967, Homo sapiens CHST2 gene for carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, without stop codon, in Flexi system.
EU832159 - Synthetic construct Homo sapiens clone HAIB:100067188; DKFZo008G0225 carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 protein (CHST2) gene, encodes complete protein.
EU832253 - Synthetic construct Homo sapiens clone HAIB:100067282; DKFZo004G0226 carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 protein (CHST2) gene, encodes complete protein.
LF213165 - JP 2014500723-A/20668: Polycomb-Associated Non-Coding RNAs.
MA448742 - JP 2018138019-A/20668: Polycomb-Associated Non-Coding RNAs.
LF378112 - JP 2014500723-A/185615: Polycomb-Associated Non-Coding RNAs.
MA613689 - JP 2018138019-A/185615: Polycomb-Associated Non-Coding RNAs.
JD500240 - Sequence 481264 from Patent EP1572962.
JD559526 - Sequence 540550 from Patent EP1572962.
JD392353 - Sequence 373377 from Patent EP1572962.
JD147374 - Sequence 128398 from Patent EP1572962.
JD055893 - Sequence 36917 from Patent EP1572962.
LF378111 - JP 2014500723-A/185614: Polycomb-Associated Non-Coding RNAs.
MA613688 - JP 2018138019-A/185614: Polycomb-Associated Non-Coding RNAs.
JD498809 - Sequence 479833 from Patent EP1572962.
LF378110 - JP 2014500723-A/185613: Polycomb-Associated Non-Coding RNAs.
MA613687 - JP 2018138019-A/185613: Polycomb-Associated Non-Coding RNAs.
LF378109 - JP 2014500723-A/185612: Polycomb-Associated Non-Coding RNAs.
MA613686 - JP 2018138019-A/185612: Polycomb-Associated Non-Coding RNAs.
LF378108 - JP 2014500723-A/185611: Polycomb-Associated Non-Coding RNAs.
MA613685 - JP 2018138019-A/185611: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00533 - Glycosaminoglycan biosynthesis - keratan sulfate
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY-6567 - chondroitin sulfate biosynthesis (late stages)
PWY-6569 - chondroitin sulfate biosynthesis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000309575.1, ENST00000309575.2, ENST00000309575.3, ENST00000309575.4, NM_004267, uc003evm.1, uc003evm.2, uc003evm.3, uc003evm.4, uc003evm.5, V9HVX9
UCSC ID: ENST00000309575.5
RefSeq Accession: NM_004267
CCDS: CCDS3129.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.