Human Gene MAN2B2 (ENST00000285599.8) from GENCODE V44
  Description: Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA. (from RefSeq NM_015274)
Gencode Transcript: ENST00000285599.8
Gencode Gene: ENSG00000013288.9
Transcript (Including UTRs)
   Position: hg38 chr4:6,575,189-6,623,362 Size: 48,174 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg38 chr4:6,575,211-6,621,285 Size: 46,075 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:6,575,189-6,623,362)mRNA (may differ from genome)Protein (1009 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MA2B2_HUMAN
DESCRIPTION: RecName: Full=Epididymis-specific alpha-mannosidase; EC=3.2.1.24; AltName: Full=Mannosidase alpha class 2B member 2; Flags: Precursor;
CATALYTIC ACTIVITY: Hydrolysis of terminal, non-reducing alpha-D- mannose residues in alpha-D-mannosides.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBCELLULAR LOCATION: Secreted (Potential).
SIMILARITY: Belongs to the glycosyl hydrolase 38 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.50 RPKM in Artery - Aorta
Total median expression: 425.11 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -5.2022-0.236 Picture PostScript Text
3' UTR -827.802077-0.399 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011013 - Glyco_hydro-type_carb-bd
IPR011330 - Glyco_hydro/deAcase_b/a-brl
IPR013780 - Glyco_hydro_13_b
IPR011682 - Glyco_hydro_38_C
IPR015341 - Glyco_hydro_38_cen_dom
IPR000602 - Glyco_hydro_38_core

Pfam Domains:
PF09261 - Alpha mannosidase middle domain
PF01074 - Glycosyl hydrolases family 38 N-terminal domain
PF07748 - Glycosyl hydrolases family 38 C-terminal domain

ModBase Predicted Comparative 3D Structure on Q9Y2E5
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004559 alpha-mannosidase activity
GO:0008496 mannan endo-1,6-alpha-mannosidase activity
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0030246 carbohydrate binding
GO:0046872 metal ion binding

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0006013 mannose metabolic process
GO:0006517 protein deglycosylation
GO:0008152 metabolic process
GO:0009313 oligosaccharide catabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0043202 lysosomal lumen
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF206820 - JP 2014500723-A/14323: Polycomb-Associated Non-Coding RNAs.
MA442397 - JP 2018138019-A/14323: Polycomb-Associated Non-Coding RNAs.
BC094773 - Homo sapiens mannosidase, alpha, class 2B, member 2, mRNA (cDNA clone MGC:104672 IMAGE:30531368), complete cds.
AK301473 - Homo sapiens cDNA FLJ61716 complete cds, highly similar to Epididymis-specific alpha-mannosidase precursor(EC 3.2.1.24).
JD193911 - Sequence 174935 from Patent EP1572962.
JD271251 - Sequence 252275 from Patent EP1572962.
AL832232 - Homo sapiens mRNA; cDNA DKFZp686F0253 (from clone DKFZp686F0253).
AL833071 - Homo sapiens mRNA; cDNA DKFZp451A0519 (from clone DKFZp451A0519); complete cds.
BC033307 - Homo sapiens mannosidase, alpha, class 2B, member 2, mRNA (cDNA clone MGC:24942 IMAGE:4932398), complete cds.
GQ891475 - Homo sapiens clone HEL-S-196 epididymis secretory sperm binding protein mRNA, complete cds.
KJ898474 - Synthetic construct Homo sapiens clone ccsbBroadEn_07868 MAN2B2-like gene, encodes complete protein.
AB023152 - Homo sapiens mRNA for KIAA0935 protein, partial cds.
LF358263 - JP 2014500723-A/165766: Polycomb-Associated Non-Coding RNAs.
MA593840 - JP 2018138019-A/165766: Polycomb-Associated Non-Coding RNAs.
LF358262 - JP 2014500723-A/165765: Polycomb-Associated Non-Coding RNAs.
MA593839 - JP 2018138019-A/165765: Polycomb-Associated Non-Coding RNAs.
AK025803 - Homo sapiens cDNA: FLJ22150 fis, clone HRC00109.
LF358261 - JP 2014500723-A/165764: Polycomb-Associated Non-Coding RNAs.
MA593838 - JP 2018138019-A/165764: Polycomb-Associated Non-Coding RNAs.
BC036836 - Homo sapiens mannosidase, alpha, class 2B, member 2, mRNA (cDNA clone IMAGE:4993533), complete cds.
LF358260 - JP 2014500723-A/165763: Polycomb-Associated Non-Coding RNAs.
MA593837 - JP 2018138019-A/165763: Polycomb-Associated Non-Coding RNAs.
AK075246 - Homo sapiens cDNA FLJ90765 fis, clone THYRO1000584, highly similar to Epididymis-specific alpha-mannosidase precursor (EC 3.2.1.24).
LF358259 - JP 2014500723-A/165762: Polycomb-Associated Non-Coding RNAs.
MA593836 - JP 2018138019-A/165762: Polycomb-Associated Non-Coding RNAs.
LF358256 - JP 2014500723-A/165759: Polycomb-Associated Non-Coding RNAs.
MA593833 - JP 2018138019-A/165759: Polycomb-Associated Non-Coding RNAs.
JD296540 - Sequence 277564 from Patent EP1572962.
JD308031 - Sequence 289055 from Patent EP1572962.
JD112578 - Sequence 93602 from Patent EP1572962.
JD490805 - Sequence 471829 from Patent EP1572962.
JD481942 - Sequence 462966 from Patent EP1572962.
LF358255 - JP 2014500723-A/165758: Polycomb-Associated Non-Coding RNAs.
MA593832 - JP 2018138019-A/165758: Polycomb-Associated Non-Coding RNAs.
JD353544 - Sequence 334568 from Patent EP1572962.
JD260994 - Sequence 242018 from Patent EP1572962.
JD104425 - Sequence 85449 from Patent EP1572962.
JD103348 - Sequence 84372 from Patent EP1572962.
JD039282 - Sequence 20306 from Patent EP1572962.
JD049354 - Sequence 30378 from Patent EP1572962.
JD531020 - Sequence 512044 from Patent EP1572962.
JD420522 - Sequence 401546 from Patent EP1572962.
JD189160 - Sequence 170184 from Patent EP1572962.
JD373880 - Sequence 354904 from Patent EP1572962.
JD224750 - Sequence 205774 from Patent EP1572962.
JD318343 - Sequence 299367 from Patent EP1572962.
JD352572 - Sequence 333596 from Patent EP1572962.
JD524775 - Sequence 505799 from Patent EP1572962.
JD414570 - Sequence 395594 from Patent EP1572962.
JD337754 - Sequence 318778 from Patent EP1572962.
JD463315 - Sequence 444339 from Patent EP1572962.
JD426724 - Sequence 407748 from Patent EP1572962.
JD286934 - Sequence 267958 from Patent EP1572962.
JD067421 - Sequence 48445 from Patent EP1572962.
JD397764 - Sequence 378788 from Patent EP1572962.
JD416241 - Sequence 397265 from Patent EP1572962.
JD242708 - Sequence 223732 from Patent EP1572962.
JD330999 - Sequence 312023 from Patent EP1572962.
JD287300 - Sequence 268324 from Patent EP1572962.
JD341818 - Sequence 322842 from Patent EP1572962.
JD479569 - Sequence 460593 from Patent EP1572962.
JD395012 - Sequence 376036 from Patent EP1572962.
JD560671 - Sequence 541695 from Patent EP1572962.
JD476692 - Sequence 457716 from Patent EP1572962.
JD414729 - Sequence 395753 from Patent EP1572962.
JD120507 - Sequence 101531 from Patent EP1572962.
JD379589 - Sequence 360613 from Patent EP1572962.
JD094075 - Sequence 75099 from Patent EP1572962.
JD311554 - Sequence 292578 from Patent EP1572962.
JD356269 - Sequence 337293 from Patent EP1572962.
JD505683 - Sequence 486707 from Patent EP1572962.
JD553757 - Sequence 534781 from Patent EP1572962.
JD544510 - Sequence 525534 from Patent EP1572962.
JD371060 - Sequence 352084 from Patent EP1572962.
JD355351 - Sequence 336375 from Patent EP1572962.
JD330030 - Sequence 311054 from Patent EP1572962.
JD324000 - Sequence 305024 from Patent EP1572962.
JD483471 - Sequence 464495 from Patent EP1572962.
JD232030 - Sequence 213054 from Patent EP1572962.
JD279562 - Sequence 260586 from Patent EP1572962.
JD489434 - Sequence 470458 from Patent EP1572962.
JD500064 - Sequence 481088 from Patent EP1572962.
JD288490 - Sequence 269514 from Patent EP1572962.
JD148383 - Sequence 129407 from Patent EP1572962.
JD463900 - Sequence 444924 from Patent EP1572962.
JD478652 - Sequence 459676 from Patent EP1572962.
JD279210 - Sequence 260234 from Patent EP1572962.
JD223335 - Sequence 204359 from Patent EP1572962.
JD286822 - Sequence 267846 from Patent EP1572962.
JD511607 - Sequence 492631 from Patent EP1572962.
JD319922 - Sequence 300946 from Patent EP1572962.
JD319923 - Sequence 300947 from Patent EP1572962.
JD319924 - Sequence 300948 from Patent EP1572962.
JD376771 - Sequence 357795 from Patent EP1572962.
JD411971 - Sequence 392995 from Patent EP1572962.
JD257010 - Sequence 238034 from Patent EP1572962.
JD441598 - Sequence 422622 from Patent EP1572962.
JD329546 - Sequence 310570 from Patent EP1572962.
JD172917 - Sequence 153941 from Patent EP1572962.
JD321982 - Sequence 303006 from Patent EP1572962.
JD398227 - Sequence 379251 from Patent EP1572962.
JD417051 - Sequence 398075 from Patent EP1572962.
JD520394 - Sequence 501418 from Patent EP1572962.
JD129276 - Sequence 110300 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00511 - Other glycan degradation

Reactome (by CSHL, EBI, and GO)

Protein Q9Y2E5 (Reactome details) participates in the following event(s):

R-HSA-6799581 MAN2B2 hydrolyses GlcNAc (Man)3 to GlcNAc:Man
R-HSA-8853383 Lysosomal oligosaccharide catabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000285599.1, ENST00000285599.2, ENST00000285599.3, ENST00000285599.4, ENST00000285599.5, ENST00000285599.6, ENST00000285599.7, KIAA0935, MA2B2_HUMAN, NM_015274, Q66MP2, Q86T67, Q9Y2E5, uc003gjf.1, uc003gjf.2, uc003gjf.3
UCSC ID: ENST00000285599.8
RefSeq Accession: NM_015274
Protein: Q9Y2E5 (aka MA2B2_HUMAN or M2B2_HUMAN)
CCDS: CCDS33951.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.