Human Gene ACOX3 (ENST00000356406.10) from GENCODE V44
  Description: Homo sapiens acyl-CoA oxidase 3, pristanoyl (ACOX3), transcript variant 1, mRNA. (from RefSeq NM_003501)
RefSeq Summary (NM_003501): Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000356406.10
Gencode Gene: ENSG00000087008.16
Transcript (Including UTRs)
   Position: hg38 chr4:8,366,282-8,440,723 Size: 74,442 Total Exon Count: 18 Strand: -
Coding Region
   Position: hg38 chr4:8,366,961-8,416,521 Size: 49,561 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:8,366,282-8,440,723)mRNA (may differ from genome)Protein (700 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ACOX3_HUMAN
DESCRIPTION: RecName: Full=Peroxisomal acyl-coenzyme A oxidase 3; EC=1.3.3.6; AltName: Full=Branched-chain acyl-CoA oxidase; Short=BRCACox; AltName: Full=Pristanoyl-CoA oxidase;
FUNCTION: Oxidizes the CoA-esters of 2-methyl-branched fatty acids (By similarity).
CATALYTIC ACTIVITY: Acyl-CoA + O(2) = trans-2,3-dehydroacyl-CoA + H(2)O(2).
COFACTOR: FAD (By similarity).
PATHWAY: Lipid metabolism; peroxisomal fatty acid beta-oxidation.
SUBCELLULAR LOCATION: Peroxisome (Potential).
SIMILARITY: Belongs to the acyl-CoA oxidase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • C576882 1-(2-trifluoromethoxyphenyl)-2-nitroethanone
  • C524733 17-ethynyl-5-androstene-3, 7, 17-triol
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C472791 3-(4'-hydroxy-3'-adamantylbiphenyl-4-yl)acrylic acid
  • D000082 Acetaminophen
  • D000643 Ammonium Chloride
  • D002117 Calcitriol
  • D002251 Carbon Tetrachloride
  • D008694 Methamphetamine
          more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.17 RPKM in Esophagus - Mucosa
Total median expression: 285.53 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -26.3090-0.292 Picture PostScript Text
3' UTR -187.00679-0.275 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006090 - Acyl-CoA_Oxase/DH_1
IPR006091 - Acyl-CoA_Oxase/DH_cen-dom
IPR012258 - Acyl-CoA_oxidase
IPR002655 - Acyl-CoA_oxidase_C
IPR009075 - AcylCo_DH/oxidase_C
IPR009100 - AcylCoA_DH/oxidase

Pfam Domains:
PF01756 - Acyl-CoA oxidase
PF00441 - Acyl-CoA dehydrogenase, C-terminal domain
PF02770 - Acyl-CoA dehydrogenase, middle domain

ModBase Predicted Comparative 3D Structure on O15254
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003995 acyl-CoA dehydrogenase activity
GO:0003997 acyl-CoA oxidase activity
GO:0005102 receptor binding
GO:0016402 pristanoyl-CoA oxidase activity
GO:0016491 oxidoreductase activity
GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
GO:0050660 flavin adenine dinucleotide binding
GO:0071949 FAD binding

Biological Process:
GO:0006625 protein targeting to peroxisome
GO:0006629 lipid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006635 fatty acid beta-oxidation
GO:0033539 fatty acid beta-oxidation using acyl-CoA dehydrogenase
GO:0033540 fatty acid beta-oxidation using acyl-CoA oxidase
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005777 peroxisome
GO:0005782 peroxisomal matrix
GO:0005829 cytosol
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AK225080 - Homo sapiens mRNA for ACOX3 protein variant, clone: CAS04861.
BC017053 - Homo sapiens acyl-Coenzyme A oxidase 3, pristanoyl, mRNA (cDNA clone MGC:9319 IMAGE:3915484), complete cds.
Y11411 - H.sapiens mRNA for pristanoyl-CoA oxidase.
AK313242 - Homo sapiens cDNA, FLJ93747, highly similar to Homo sapiens acyl-Coenzyme A oxidase 3, pristanoyl (ACOX3), mRNA.
KJ892494 - Synthetic construct Homo sapiens clone ccsbBroadEn_01888 ACOX3 gene, encodes complete protein.
JD408207 - Sequence 389231 from Patent EP1572962.
JD230790 - Sequence 211814 from Patent EP1572962.
JD058339 - Sequence 39363 from Patent EP1572962.
DQ585076 - Homo sapiens piRNA piR-52188, complete sequence.
CU677813 - Synthetic construct Homo sapiens gateway clone IMAGE:100020397 5' read ACOX3 mRNA.
JD552895 - Sequence 533919 from Patent EP1572962.
JD429681 - Sequence 410705 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00071 - Fatty acid metabolism
hsa00592 - alpha-Linolenic acid metabolism
hsa01040 - Biosynthesis of unsaturated fatty acids
hsa01100 - Metabolic pathways
hsa03320 - PPAR signaling pathway
hsa04146 - Peroxisome

Reactome (by CSHL, EBI, and GO)

Protein O15254 (Reactome details) participates in the following event(s):

R-HSA-9033233 PEX5S,L binds cargo proteins containing PTS1
R-HSA-389891 (2S)-pristanoyl-CoA + O2 => trans-2,3-dehydropristanoyl-CoA + H2O2 (ACOX3)
R-HSA-9033236 PEX5S,L:Cargo binds PEX13:PEX14 of PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Complex)
R-HSA-9033241 Peroxisomal protein import
R-HSA-389887 Beta-oxidation of pristanoyl-CoA
R-HSA-392499 Metabolism of proteins
R-HSA-390918 Peroxisomal lipid metabolism
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ACOX3_HUMAN, BRCOX, ENST00000356406.1, ENST00000356406.2, ENST00000356406.3, ENST00000356406.4, ENST00000356406.5, ENST00000356406.6, ENST00000356406.7, ENST00000356406.8, ENST00000356406.9, NM_003501, O15254, PRCOX, Q96AJ8, uc003glc.1, uc003glc.2, uc003glc.3, uc003glc.4, uc003glc.5, uc003glc.6
UCSC ID: ENST00000356406.10
RefSeq Accession: NM_003501
Protein: O15254 (aka ACOX3_HUMAN or CAO3_HUMAN)
CCDS: CCDS3401.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.