Human Gene SPEF2 (ENST00000282469.10) from GENCODE V44
  Description: Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA. (from RefSeq NM_144722)
Gencode Transcript: ENST00000282469.10
Gencode Gene: ENSG00000152582.14
Transcript (Including UTRs)
   Position: hg38 chr5:35,617,844-35,671,575 Size: 53,732 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg38 chr5:35,617,998-35,670,248 Size: 52,251 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:35,617,844-35,671,575)mRNA (may differ from genome)Protein (514 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SPEF2_HUMAN
DESCRIPTION: RecName: Full=Sperm flagellar protein 2; AltName: Full=Protein KPL2;
FUNCTION: Required for correct axoneme development (By similarity).
SIMILARITY: Contains 1 CH (calponin-homology) domain.
SEQUENCE CAUTION: Sequence=BAB15700.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.77 RPKM in Cervix - Endocervix
Total median expression: 56.01 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -59.10154-0.384 Picture PostScript Text
3' UTR -267.501327-0.202 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000850 - Adenylate_kin
IPR001715 - CH-domain
IPR010441 - DUF1042
IPR008906 - HATC

Pfam Domains:
PF00406 - Adenylate kinase
PF05699 - hAT family C-terminal dimerisation region
PF06294 - CH-like domain in sperm protein

ModBase Predicted Comparative 3D Structure on Q9C093
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Descriptions from all associated GenBank mRNAs
  BC067761 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:5298423), containing frame-shift errors.
BC016773 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:4092589), with apparent retained intron.
EF553528 - Homo sapiens clone DKFZp686K07130 FLJ23577 mRNA sequence, alternatively spliced.
HM005670 - Homo sapiens clone HTL-T-47a testis tissue sperm-binding protein Li 47a mRNA, complete cds.
AK058124 - Homo sapiens cDNA FLJ25395 fis, clone TST02553.
BC110984 - Homo sapiens sperm flagellar 2, mRNA (cDNA clone IMAGE:5163398), complete cds.
BC156528 - Synthetic construct Homo sapiens clone IMAGE:100063160, MGC:190710 sperm flagellar 2 (SPEF2) mRNA, encodes complete protein.
BC172520 - Synthetic construct Homo sapiens clone IMAGE:100069214, MGC:199225 sperm flagellar 2 (SPEF2) mRNA, encodes complete protein.
AL832607 - Homo sapiens mRNA; cDNA DKFZp451H1817 (from clone DKFZp451H1817).

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000282469.1, ENST00000282469.2, ENST00000282469.3, ENST00000282469.4, ENST00000282469.5, ENST00000282469.6, ENST00000282469.7, ENST00000282469.8, ENST00000282469.9, KIAA1770, KPL2, NM_144722, Q2TAC9, Q96LL6, Q9C093, Q9H5C7, Q9H5Q7, SPEF2_HUMAN, uc003jjn.1, uc003jjn.2
UCSC ID: ENST00000282469.10
RefSeq Accession: NM_144722
Protein: Q9C093 (aka SPEF2_HUMAN)
CCDS: CCDS3910.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.