Human Gene KLC4 (ENST00000259708.7) from GENCODE V44
  Description: Homo sapiens kinesin light chain 4 (KLC4), transcript variant 3, mRNA. (from RefSeq NM_201523)
Gencode Transcript: ENST00000259708.7
Gencode Gene: ENSG00000137171.15
Transcript (Including UTRs)
   Position: hg38 chr6:43,059,857-43,075,093 Size: 15,237 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg38 chr6:43,060,210-43,074,672 Size: 14,463 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesGeneReviewsMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:43,059,857-43,075,093)mRNA (may differ from genome)Protein (637 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGImyGene2
neXtProtPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KLC4_HUMAN
DESCRIPTION: RecName: Full=Kinesin light chain 4; Short=KLC 4; AltName: Full=Kinesin-like protein 8;
FUNCTION: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity).
SUBUNIT: Oligomeric complex composed of two heavy chains and two light chains (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (Probable).
SIMILARITY: Belongs to the kinesin light chain family.
SIMILARITY: Contains 7 TPR repeats.
SEQUENCE CAUTION: Sequence=BAC86788.1; Type=Erroneous translation; Note=Wrong choice of CDS;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.76 RPKM in Liver
Total median expression: 543.54 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -118.50353-0.336 Picture PostScript Text
3' UTR -155.50421-0.369 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002151 - Kinesin_light
IPR015792 - Kinesin_light_repeat
IPR015390 - Rabaptin_Rab5-bd_dom
IPR013026 - TPR-contain_dom
IPR011990 - TPR-like_helical
IPR013105 - TPR_2
IPR019734 - TPR_repeat

Pfam Domains:
PF09311 - Rabaptin-like protein
PF07719 - Tetratricopeptide repeat

ModBase Predicted Comparative 3D Structure on Q9NSK0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGD  WormBase 
Protein SequenceProtein Sequence  Protein Sequence 
AlignmentAlignment  Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003777 microtubule motor activity
GO:0005515 protein binding

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005871 kinesin complex
GO:0005874 microtubule


-  Descriptions from all associated GenBank mRNAs
  AK127018 - Homo sapiens cDNA FLJ45074 fis, clone BRAWH3027440, highly similar to Probable kinesin light chain 3.
AK297433 - Homo sapiens cDNA FLJ58264 complete cds, highly similar to Kinesin light chain 4.
AK056393 - Homo sapiens cDNA FLJ31831 fis, clone NT2RP6000123, highly similar to Kinesin light chain 4.
BC012357 - Homo sapiens kinesin light chain 4, mRNA (cDNA clone IMAGE:4654235), complete cds.
BC080637 - Homo sapiens kinesin light chain 4, mRNA (cDNA clone MGC:99549 IMAGE:6573021), complete cds.
BC103727 - Homo sapiens kinesin light chain 4, mRNA (cDNA clone MGC:111777 IMAGE:6157949), complete cds.
AK094102 - Homo sapiens cDNA FLJ36783 fis, clone ADRGL2001830, highly similar to Kinesin light chain 4.
AK055293 - Homo sapiens cDNA FLJ30731 fis, clone FEBRA2000105, highly similar to Kinesin light chain 4.
BX640717 - Homo sapiens mRNA; cDNA DKFZp686J01192 (from clone DKFZp686J01192).
AK127894 - Homo sapiens cDNA FLJ46000 fis, clone SMINT2010753, highly similar to Probable kinesin light chain 3.
DQ895432 - Synthetic construct Homo sapiens clone IMAGE:100009892; FLH184347.01L; RZPDo839E12143D kinesin light chain 4 (KLC4) gene, encodes complete protein.
DQ892231 - Synthetic construct clone IMAGE:100004861; FLH184351.01X; RZPDo839E12144D kinesin light chain 4 (KLC4) gene, encodes complete protein.
EU831841 - Synthetic construct Homo sapiens clone HAIB:100066870; DKFZo004B0722 kinesin light chain 4 protein (KLC4) gene, encodes complete protein.
EU831763 - Synthetic construct Homo sapiens clone HAIB:100066792; DKFZo008B0721 kinesin light chain 4 protein (KLC4) gene, encodes complete protein.
AL162078 - Homo sapiens mRNA; cDNA DKFZp761H229 (from clone DKFZp761H229); partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NSK0 (Reactome details) participates in the following event(s):

R-HSA-983194 Kinesin-1 is a heterotetramer
R-HSA-5672083 KTN1 binds Kinesin-1
R-HSA-983266 Kinesins bind microtubules
R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules
R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs
R-HSA-983189 Kinesins
R-HSA-5625970 RHO GTPases activate KTN1
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-195258 RHO GTPase Effectors
R-HSA-109582 Hemostasis
R-HSA-2132295 MHC class II antigen presentation
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-194315 Signaling by Rho GTPases
R-HSA-1280218 Adaptive Immune System
R-HSA-8856688 Golgi-to-ER retrograde transport
R-HSA-162582 Signal Transduction
R-HSA-168256 Immune System
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: B3KNY4, B3KPI3, B3KSQ3, ENST00000259708.1, ENST00000259708.2, ENST00000259708.3, ENST00000259708.4, ENST00000259708.5, ENST00000259708.6, KLC4_HUMAN, KNSL8, NM_201523, Q66K28, Q96EG6, Q9NSK0, uc003otw.1, uc003otw.2, uc003otw.3
UCSC ID: ENST00000259708.7
RefSeq Accession: NM_201523
Protein: Q9NSK0 (aka KLC4_HUMAN)
CCDS: CCDS4883.1, CCDS4882.1

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene KLC4:
hsp (Hereditary Spastic Paraplegia Overview)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.