Human Gene ZP3 (ENST00000394857.8) from GENCODE V44
  Description: Homo sapiens zona pellucida glycoprotein 3 (ZP3), transcript variant 1, mRNA. (from RefSeq NM_001110354)
RefSeq Summary (NM_001110354): The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000394857.8
Gencode Gene: ENSG00000188372.15
Transcript (Including UTRs)
   Position: hg38 chr7:76,424,950-76,442,069 Size: 17,120 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr7:76,424,965-76,442,056 Size: 17,092 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:76,424,950-76,442,069)mRNA (may differ from genome)Protein (424 aa)
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-  Comments and Description Text from UniProtKB
  ID: ZP3_HUMAN
DESCRIPTION: RecName: Full=Zona pellucida sperm-binding protein 3; AltName: Full=Sperm receptor; AltName: Full=ZP3A/ZP3B; AltName: Full=Zona pellucida glycoprotein 3; Short=Zp-3; AltName: Full=Zona pellucida protein C; Contains: RecName: Full=Processed zona pellucida sperm-binding protein 3; Flags: Precursor;
FUNCTION: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
SUBUNIT: Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity).
SUBCELLULAR LOCATION: Processed zona pellucida sperm-binding protein 3: Secreted, extracellular space, extracellular matrix.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Oocytes.
DOMAIN: The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
PTM: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
PTM: N-glycosylated (By similarity).
PTM: O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy (By similarity).
SIMILARITY: Belongs to the ZP domain family. ZPC subfamily.
SIMILARITY: Contains 1 ZP domain.
WEB RESOURCE: Name=Protein Spotlight; Note=Molecular chastity - Issue 93 of April 2008; URL="http://web.expasy.org/spotlight/back_issues/sptlt093.shtml";

-  Primer design for this transcript
 

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-  MalaCards Disease Associations
  MalaCards Gene Search: ZP3
Diseases sorted by gene-association score: infertility (7), ovarian disease (6), retinitis pigmentosa 27 (3), reproductive system disease (2), female reproductive system disease (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.14 RPKM in Testis
Total median expression: 76.51 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001507 - ZP_dom
IPR017977 - ZP_dom_CS

Pfam Domains:
PF00100 - Zona pellucida-like domain

ModBase Predicted Comparative 3D Structure on P21754
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0030246 carbohydrate binding
GO:0032190 acrosin binding
GO:0042802 identical protein binding
GO:0048018 receptor agonist activity

Biological Process:
GO:0001809 positive regulation of type IV hypersensitivity
GO:0001825 blastocyst formation
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0002687 positive regulation of leukocyte migration
GO:0002922 positive regulation of humoral immune response
GO:0007338 single fertilization
GO:0007339 binding of sperm to zona pellucida
GO:0010469 regulation of receptor activity
GO:0010513 positive regulation of phosphatidylinositol biosynthetic process
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0035803 egg coat formation
GO:0042102 positive regulation of T cell proliferation
GO:0045892 negative regulation of transcription, DNA-templated
GO:0045893 positive regulation of transcription, DNA-templated
GO:0048015 phosphatidylinositol-mediated signaling
GO:0048599 oocyte development
GO:0050729 positive regulation of inflammatory response
GO:2000344 positive regulation of acrosome reaction
GO:2000360 negative regulation of binding of sperm to zona pellucida
GO:2000368 positive regulation of acrosomal vesicle exocytosis
GO:2000386 positive regulation of ovarian follicle development
GO:2000388 positive regulation of antral ovarian follicle growth

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005622 intracellular
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031012 extracellular matrix


-  Descriptions from all associated GenBank mRNAs
  AK292763 - Homo sapiens cDNA FLJ76082 complete cds, highly similar to Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), mRNA.
BC113949 - Homo sapiens zona pellucida glycoprotein 3 (sperm receptor), mRNA (cDNA clone MGC:117239 IMAGE:5170097), complete cds.
X56777 - Homo sapiens mRNA for ZP3 gene.
M60504 - Homo sapiens ZP3 protein (ZP3) mRNA, complete cds.
AB464425 - Synthetic construct DNA, clone: pF1KB7229, Homo sapiens ZP3 gene for zona pellucida glycoprotein 3, without stop codon, in Flexi system.
BC140332 - Synthetic construct Homo sapiens clone IMAGE:100014356, MGC:173217 zona pellucida glycoprotein 3 (sperm receptor) (ZP3) mRNA, encodes complete protein.
BC146482 - Synthetic construct Homo sapiens clone IMAGE:100015017, MGC:180178 zona pellucida glycoprotein 3 (sperm receptor) (ZP3) mRNA, encodes complete protein.
AK056788 - Homo sapiens cDNA FLJ32226 fis, clone PLACE6004380, highly similar to ZONA PELLUCIDA SPERM-BINDING PROTEIN 3A PRECURSOR.
S52509 - Homo sapiens ZP3-474 (ZP3A) mRNA, partial cds.
JD530548 - Sequence 511572 from Patent EP1572962.
JD278613 - Sequence 259637 from Patent EP1572962.
JD232593 - Sequence 213617 from Patent EP1572962.
JD547561 - Sequence 528585 from Patent EP1572962.
JD547562 - Sequence 528586 from Patent EP1572962.
JD227507 - Sequence 208531 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P21754 (Reactome details) participates in the following event(s):

R-HSA-1297338 Association of ADAM and B4GALT1 With ZP3
R-HSA-1300644 Interaction With The Zona Pellucida
R-HSA-1187000 Fertilization
R-HSA-1474165 Reproduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000394857.1, ENST00000394857.2, ENST00000394857.3, ENST00000394857.4, ENST00000394857.5, ENST00000394857.6, ENST00000394857.7, NM_001110354, P21754, Q06633, Q29RW0, uc003ufd.1, uc003ufd.2, uc003ufd.3, uc003ufd.4, uc003ufd.5, uc003ufd.6, ZP3A, ZP3B, ZP3_HUMAN, ZPC
UCSC ID: ENST00000394857.8
RefSeq Accession: NM_001110354
Protein: P21754 (aka ZP3_HUMAN)
CCDS: CCDS47618.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.