Human Gene EPHX2 (ENST00000521400.6) from GENCODE V44
Description: Homo sapiens epoxide hydrolase 2 (EPHX2), transcript variant 1, mRNA. (from RefSeq NM_001979) RefSeq Summary (NM_001979): This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]. Gencode Transcript: ENST00000521400.6 Gencode Gene: ENSG00000120915.14 Transcript (Including UTRs) Position: hg38 chr8:27,491,143-27,545,564 Size: 54,422 Total Exon Count: 19 Strand: + Coding Region Position: hg38 chr8:27,491,209-27,544,522 Size: 53,314 Coding Exon Count: 19
ID:HYES_HUMAN DESCRIPTION: RecName: Full=Bifunctional epoxide hydrolase 2; Includes: RecName: Full=Cytosolic epoxide hydrolase 2; Short=CEH; EC=3.3.2.10; AltName: Full=Epoxide hydratase; AltName: Full=Soluble epoxide hydrolase; Short=SEH; Includes: RecName: Full=Lipid-phosphate phosphatase; EC=3.1.3.76; FUNCTION: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. CATALYTIC ACTIVITY: An epoxide + H(2)O = a glycol. CATALYTIC ACTIVITY: (9S,10S)-10-hydroxy-9- (phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10- dihydroxyoctadecanoate + phosphate. COFACTOR: Magnesium. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=21 uM for threo-9,10-phosphonooxy-hydroxy-octadecanoic acid; KM=1.1 mM for p-nitrophenyl phosphate; Vmax=338 nmol/min/mg enzyme with threo-9,10-phosphonooxy- hydroxy-octadecanoic acid; Vmax=5.8 nmol/min/mg enzyme with p-nitrophenyl phosphate; SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. Peroxisome. INDUCTION: By compounds that cause peroxisome proliferation such as clofibrate, tiadenol and fenofibrate. DOMAIN: The N-terminal domain has phosphatase activity. The C- terminal domain has epoxide hydrolase activity. PTM: The N-terminus is blocked. PTM: The covalent modification of cysteine by 15-deoxy-Delta12,14- prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S- nitrosylation and S-palmitoylation (Probable). SIMILARITY: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/ephx2/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P34913
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006625 protein targeting to peroxisome GO:0006629 lipid metabolic process GO:0006805 xenobiotic metabolic process GO:0006874 cellular calcium ion homeostasis GO:0006954 inflammatory response GO:0008152 metabolic process GO:0008217 regulation of blood pressure GO:0009636 response to toxic substance GO:0010628 positive regulation of gene expression GO:0016311 dephosphorylation GO:0017144 drug metabolic process GO:0019373 epoxygenase P450 pathway GO:0019439 aromatic compound catabolic process GO:0042632 cholesterol homeostasis GO:0042759 long-chain fatty acid biosynthetic process GO:0046272 stilbene catabolic process GO:0046839 phospholipid dephosphorylation GO:0072593 reactive oxygen species metabolic process GO:0090181 regulation of cholesterol metabolic process GO:0097176 epoxide metabolic process
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