Human Gene WNK2 (ENST00000297954.9) from GENCODE V44
  Description: Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 1, mRNA. (from RefSeq NM_001282394)
RefSeq Summary (NM_001282394): The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013].
Gencode Transcript: ENST00000297954.9
Gencode Gene: ENSG00000165238.17
Transcript (Including UTRs)
   Position: hg38 chr9:93,184,122-93,319,205 Size: 135,084 Total Exon Count: 31 Strand: +
Coding Region
   Position: hg38 chr9:93,184,930-93,318,027 Size: 133,098 Coding Exon Count: 30 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:93,184,122-93,319,205)mRNA (may differ from genome)Protein (2297 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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HGNCHPRDMalacardsMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WNK2_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase WNK2; EC=2.7.11.1; AltName: Full=Antigen NY-CO-43; AltName: Full=Protein kinase lysine-deficient 2; AltName: Full=Protein kinase with no lysine 2; AltName: Full=Serologically defined colon cancer antigen 43;
FUNCTION: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activation requires autophosphorylation of Ser- 356. Phosphorylation of Ser-352 also promotes increased activity (By similarity).
SUBUNIT: Forms a complex with the phosphorylated form of STK39 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane.
TISSUE SPECIFICITY: Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.
PTM: Autophosphorylated.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
SIMILARITY: Contains 1 protein kinase domain.
CAUTION: Cys-224 is present instead of the conserved Lys which is expected to be an active site residue. Lys-207 appears to fulfill the required catalytic function.
SEQUENCE CAUTION: Sequence=AAH37965.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: WNK2
Diseases sorted by gene-association score: gastric cardia carcinoma (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 27.36 RPKM in Heart - Atrial Appendage
Total median expression: 307.69 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -141.40266-0.532 Picture PostScript Text
3' UTR -418.871178-0.356 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR024678 - Kinase_OSR1/WNK_CCT
IPR000719 - Prot_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF12202 - Oxidative-stress-responsive kinase 1 C-terminal domain
PF00069 - Protein kinase domain

ModBase Predicted Comparative 3D Structure on Q9Y3S1
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0008285 negative regulation of cell proliferation
GO:0016310 phosphorylation
GO:0035556 intracellular signal transduction
GO:0046777 protein autophosphorylation
GO:0050801 ion homeostasis
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0090263 positive regulation of canonical Wnt signaling pathway
GO:2000651 positive regulation of sodium ion transmembrane transporter activity

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BC172768 - Synthetic construct Homo sapiens clone IMAGE:9094254 WNK lysine deficient protein kinase 2 (WNK2) gene, partial cds.
AJ242724 - Homo sapiens mRNA for putative mitogen-activated protein kinase kinase kinase (PRKWNK2 gene), splice variant KIAA1760-related.
AB044546 - Homo sapiens P/OKcl.13 mRNA for mitogen-activated protein kinase kinase kinase, partial cds.
AB051547 - Homo sapiens mRNA for KIAA1760 protein, partial cds.
AB463351 - Synthetic construct DNA, clone: pF1KA1760, Homo sapiens WNK2 gene for WNK lysine deficient protein kinase 2, without stop codon, in Flexi system.
JD474004 - Sequence 455028 from Patent EP1572962.
JD128439 - Sequence 109463 from Patent EP1572962.
JD125185 - Sequence 106209 from Patent EP1572962.
JD336051 - Sequence 317075 from Patent EP1572962.
AK310390 - Homo sapiens cDNA, FLJ17432.
AK096968 - Homo sapiens cDNA FLJ39649 fis, clone SMINT2004909, moderately similar to Homo sapiens mRNA for mitogen-activated protein kinase kinase kinase.
BC051027 - Homo sapiens mRNA similar to serologically defined colon cancer antigen 43 (cDNA clone IMAGE:4417441).
JD318793 - Sequence 299817 from Patent EP1572962.
JD493504 - Sequence 474528 from Patent EP1572962.
JD462050 - Sequence 443074 from Patent EP1572962.
JD199979 - Sequence 181003 from Patent EP1572962.
JD068303 - Sequence 49327 from Patent EP1572962.
BC172769 - Synthetic construct Homo sapiens clone IMAGE:9094255 WNK lysine deficient protein kinase 2 (WNK2) gene, partial cds.
JD115012 - Sequence 96036 from Patent EP1572962.
JD086004 - Sequence 67028 from Patent EP1572962.
AK000694 - Homo sapiens cDNA FLJ20687 fis, clone KAIA302, highly similar to AF039702 Homo sapiens antigen NY-CO-43 mRNA.
AF039702 - Homo sapiens antigen NY-CO-43 mRNA, complete cds.
BC172770 - Synthetic construct Homo sapiens clone IMAGE:9094256 WNK lysine deficient protein kinase 2 (WNK2) gene, partial cds.
BC037965 - Homo sapiens WNK lysine deficient protein kinase 2, mRNA (cDNA clone IMAGE:5735496), partial cds.
AK124314 - Homo sapiens cDNA FLJ42323 fis, clone TRACH2025344.
AK093910 - Homo sapiens cDNA FLJ36591 fis, clone TRACH2014045.
JD165967 - Sequence 146991 from Patent EP1572962.
JD048965 - Sequence 29989 from Patent EP1572962.
JD314149 - Sequence 295173 from Patent EP1572962.
JD441030 - Sequence 422054 from Patent EP1572962.
JD140530 - Sequence 121554 from Patent EP1572962.
JD450679 - Sequence 431703 from Patent EP1572962.
JD381656 - Sequence 362680 from Patent EP1572962.
JD508996 - Sequence 490020 from Patent EP1572962.
JD549966 - Sequence 530990 from Patent EP1572962.
JD479763 - Sequence 460787 from Patent EP1572962.
JD226467 - Sequence 207491 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000297954.1, ENST00000297954.2, ENST00000297954.3, ENST00000297954.4, ENST00000297954.5, ENST00000297954.6, ENST00000297954.7, ENST00000297954.8, KIAA1760, NM_001282394, P/OKcl.13, PRKWNK2, Q5VWF1, Q5VWF2, Q8IY36, Q9C0A3, Q9H3P4, Q9Y3S1, SDCCAG43, uc004ati.1, uc004ati.2, uc004ati.3, uc004ati.4, WNK2_HUMAN
UCSC ID: ENST00000297954.9
RefSeq Accession: NM_001282394
Protein: Q9Y3S1 (aka WNK2_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.