Human Gene CFAP77 (ENST00000393216.3) from GENCODE V44
  Description: Homo sapiens cilia and flagella associated protein 77 (CFAP77), transcript variant 2, mRNA. (from RefSeq NM_001282957)
Gencode Transcript: ENST00000393216.3
Gencode Gene: ENSG00000188523.9
Transcript (Including UTRs)
   Position: hg38 chr9:132,410,211-132,573,319 Size: 163,109 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr9:132,410,272-132,572,510 Size: 162,239 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:132,410,211-132,573,319)mRNA (may differ from genome)Protein (284 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
LynxMalacardsMGIneXtProtPubMedUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CI171_HUMAN
DESCRIPTION: RecName: Full=Uncharacterized protein C9orf171;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.01 RPKM in Testis
Total median expression: 22.65 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.2061-0.380 Picture PostScript Text
3' UTR -213.10809-0.263 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  ModBase Predicted Comparative 3D Structure on Q6ZQR2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Cellular Component:
GO:0005929 cilium
GO:0042995 cell projection


-  Descriptions from all associated GenBank mRNAs
  AK128819 - Homo sapiens cDNA FLJ46082 fis, clone TESTI2005153.
BC118593 - Homo sapiens chromosome 9 open reading frame 171, mRNA (cDNA clone MGC:148067 IMAGE:40108008), complete cds.
BC124555 - Homo sapiens chromosome 9 open reading frame 171, mRNA (cDNA clone MGC:148068 IMAGE:40108012), complete cds.
KJ904268 - Synthetic construct Homo sapiens clone ccsbBroadEn_13662 C9orf171 gene, encodes complete protein.
KJ905101 - Synthetic construct Homo sapiens clone ccsbBroadEn_14495 C9orf171 gene, encodes complete protein.

-  Other Names for This Gene
  Alternate Gene Symbols: C9orf171, CI171_HUMAN, ENST00000393216.1, ENST00000393216.2, NM_001282957, Q147X1, Q6ZQR2, uc004cbo.1, uc004cbo.2, uc004cbo.3, uc004cbo.4, uc004cbo.5, uc004cbo.6
UCSC ID: ENST00000393216.3
RefSeq Accession: NM_001282957
Protein: Q6ZQR2 (aka CI171_HUMAN)
CCDS: CCDS65167.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.