ID:SKA2_HUMAN DESCRIPTION: RecName: Full=Spindle and kinetochore-associated protein 2; AltName: Full=Protein FAM33A; FUNCTION: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. SUBUNIT: Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3. Forms a heterodimer with SKA1; the heterodimer interacting with SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer. The core SKA1 complex associates with microtubules and forms oligomeric assemblies. Interacts directly with SKA1. Binds directly to microtubules; but with a much lower affinity than SKA1. May interact with NR3C1; the relevance of such interaction remains unclear in vivo. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. SIMILARITY: Belongs to the SKA2 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8WVK7
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Mouse
Rat
Zebrafish
D. melanogaster
C. elegans
S. cerevisiae
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
Gene Ontology (GO) Annotations with Structured Vocabulary
LF384789 - JP 2014500723-A/192292: Polycomb-Associated Non-Coding RNAs. MA620366 - JP 2018138019-A/192292: Polycomb-Associated Non-Coding RNAs. BC106003 - Homo sapiens family with sequence similarity 33, member A, mRNA (cDNA clone MGC:110975 IMAGE:6143473), complete cds. AK056473 - Homo sapiens cDNA FLJ31911 fis, clone NT2RP7004751. AK022820 - Homo sapiens cDNA FLJ12758 fis, clone NT2RP2001328. BC017873 - Homo sapiens family with sequence similarity 33, member A, mRNA (cDNA clone MGC:22673 IMAGE:4685972), complete cds. HQ447776 - Synthetic construct Homo sapiens clone IMAGE:100071108; CCSB012915_02 family with sequence similarity 33, member A (FAM33A) gene, encodes complete protein. KJ896119 - Synthetic construct Homo sapiens clone ccsbBroadEn_05513 SKA2 gene, encodes complete protein. JD474460 - Sequence 455484 from Patent EP1572962. LF327223 - JP 2014500723-A/134726: Polycomb-Associated Non-Coding RNAs. MA562800 - JP 2018138019-A/134726: Polycomb-Associated Non-Coding RNAs. JD432045 - Sequence 413069 from Patent EP1572962. LF319941 - JP 2014500723-A/127444: Polycomb-Associated Non-Coding RNAs. LF327222 - JP 2014500723-A/134725: Polycomb-Associated Non-Coding RNAs. MA555518 - JP 2018138019-A/127444: Polycomb-Associated Non-Coding RNAs. MA562799 - JP 2018138019-A/134725: Polycomb-Associated Non-Coding RNAs. LF327219 - JP 2014500723-A/134722: Polycomb-Associated Non-Coding RNAs. MA562796 - JP 2018138019-A/134722: Polycomb-Associated Non-Coding RNAs. FJ648812 - Homo sapiens family with sequence similarity 33 member A (FAM33A) mRNA, partial cds.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q8WVK7 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction
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