Human Gene MOB4 (ENST00000409360.1) from GENCODE V44
  Description: Homo sapiens MOB family member 4, phocein (MOB4), transcript variant 4, mRNA. (from RefSeq NM_001204094)
RefSeq Summary (NM_001204094): This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HSPE1.[provided by RefSeq, Feb 2011].
Gencode Transcript: ENST00000409360.1
Gencode Gene: ENSG00000115540.15
Transcript (Including UTRs)
   Position: hg38 chr2:197,516,160-197,551,279 Size: 35,120 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr2:197,523,660-197,550,646 Size: 26,987 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:197,516,160-197,551,279)mRNA (may differ from genome)Protein (193 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCLynxMalacardsMGIneXtProtOMIM
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PHOCN_HUMAN
DESCRIPTION: RecName: Full=MOB-like protein phocein; AltName: Full=2C4D; AltName: Full=Class II mMOB1; AltName: Full=Mob1 homolog 3; Short=Mob3; AltName: Full=Mps one binder kinase activator-like 3; AltName: Full=Preimplantation protein 3;
FUNCTION: May play a role in membrane trafficking, specifically in membrane budding reactions (By similarity).
SUBUNIT: Binds STRN4 (By similarity). Interacts with DNM1 and EPS15 (By similarity). Interacts with nucleoside diphosphate kinase (By similarity). Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2.
INTERACTION: Q9P2B4:CTTNBP2NL; NbExp=5; IntAct=EBI-713935, EBI-1774273; O43815:STRN; NbExp=3; IntAct=EBI-713935, EBI-1046642; Q9Y228:TRAF3IP3; NbExp=2; IntAct=EBI-713935, EBI-765817;
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Note=In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.
PTM: Phosphorylated on serine residues.
SIMILARITY: Belongs to the MOB1/phocein family.
SEQUENCE CAUTION: Sequence=AAP97221.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAB45697.1; Type=Frameshift; Positions=168;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MOB4
Diseases sorted by gene-association score: myeloproliferative syndrome, transient (6), cerebral cavernous malformations 3 (5), central nervous system hemangioma (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.49 RPKM in Adrenal Gland
Total median expression: 7.80 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -108.00232-0.466 Picture PostScript Text
3' UTR -123.70633-0.195 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005301 - Mob1_phocein

Pfam Domains:
PF03637 - Mob1/phocein family

ModBase Predicted Comparative 3D Structure on Q9Y3A3
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0046872 metal ion binding

Cellular Component:
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0032580 Golgi cisterna membrane
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK289377 - Homo sapiens cDNA FLJ76596 complete cds, highly similar to Homo sapiens preimplantation protein 3 (PREI3), transcript variant 2, mRNA.
AF250319 - Homo sapiens CGI95-iso mRNA, complete cds.
BC005237 - Homo sapiens MOB1, Mps One Binder kinase activator-like 3 (yeast), mRNA (cDNA clone MGC:12264 IMAGE:3996585), complete cds.
LF383606 - JP 2014500723-A/191109: Polycomb-Associated Non-Coding RNAs.
MA619183 - JP 2018138019-A/191109: Polycomb-Associated Non-Coding RNAs.
AK292938 - Homo sapiens cDNA FLJ78747 complete cds, highly similar to Homo sapiens preimplantation protein 3 (PREI3), transcript variant 1, mRNA.
AK027043 - Homo sapiens cDNA: FLJ23390 fis, clone HEP17176.
AF093825 - Homo sapiens 2C4D mRNA, complete cds.
AF151853 - Homo sapiens CGI-95 protein mRNA, complete cds.
AK307497 - Homo sapiens cDNA, FLJ97445.
AL080070 - Homo sapiens mRNA; cDNA DKFZp564M112 (from clone DKFZp564M112); complete cds.
AB015441 - Homo sapiens mRNA for Mob3, complete cds.
AK297514 - Homo sapiens cDNA FLJ55109 complete cds, highly similar to Preimplantation protein 3.
AK297294 - Homo sapiens cDNA FLJ52887 complete cds, highly similar to Preimplantation protein 3.
CR457371 - Homo sapiens full open reading frame cDNA clone RZPDo834C1210D for gene PREI3, preimplantation protein 3; complete cds, incl. stopcodon.
AB590538 - Synthetic construct DNA, clone: pFN21AB6914, Homo sapiens MOBKL3 gene for MOB1, Mps One Binder kinase activator-like 3, without stop codon, in Flexi system.
AJ580638 - Homo sapiens mRNA for Mob1 protein (MOB1 gene).
LF317765 - JP 2014500723-A/125268: Polycomb-Associated Non-Coding RNAs.
MA553342 - JP 2018138019-A/125268: Polycomb-Associated Non-Coding RNAs.
LF317769 - JP 2014500723-A/125272: Polycomb-Associated Non-Coding RNAs.
MA553346 - JP 2018138019-A/125272: Polycomb-Associated Non-Coding RNAs.
LF317770 - JP 2014500723-A/125273: Polycomb-Associated Non-Coding RNAs.
MA553347 - JP 2018138019-A/125273: Polycomb-Associated Non-Coding RNAs.
JD315732 - Sequence 296756 from Patent EP1572962.
JD357703 - Sequence 338727 from Patent EP1572962.
JD311407 - Sequence 292431 from Patent EP1572962.
JD562563 - Sequence 543587 from Patent EP1572962.
JD049964 - Sequence 30988 from Patent EP1572962.
JD559823 - Sequence 540847 from Patent EP1572962.
JD093423 - Sequence 74447 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B4DML0, CGI-95, MOB3, MOBKL3, NM_001204094, PHOCN, PHOCN_HUMAN, PREI3, Q53SE0, Q7Z4Y6, Q9H2P3, Q9H5J1, Q9Y3A3, Q9Y4T8, uc021vun.1, uc021vun.2
UCSC ID: ENST00000409360.1
RefSeq Accession: NM_001204094
Protein: Q9Y3A3 (aka PHOCN_HUMAN)
CCDS: CCDS2322.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.