Human Gene MIR421 (ENST00000365696.4) from GENCODE V44
  Description: Homo sapiens microRNA 421 (MIR421), microRNA. (from RefSeq NR_030398)
RefSeq Summary (NR_030398): microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]. Sequence Note: This record represents a predicted microRNA stem-loop as defined by miRBase. Some sequence at the 5' and 3' ends may not be included in the intermediate precursor miRNA produced by Drosha cleavage.
Gencode Transcript: ENST00000365696.4
Gencode Gene: ENSG00000202566.4
Transcript (Including UTRs)
   Position: hg38 chrX:74,218,377-74,218,461 Size: 85 Total Exon Count: 1 Strand: -


Page IndexSequence and LinksPrimersCTDRNA-Seq ExpressionMicroarray Expression
Other SpeciesmRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:74,218,377-74,218,461)mRNA (may differ from genome)No protein
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
EnsemblEntrez GeneExonPrimerGencodeHGNCLynx
PubMedBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.28 RPKM in Brain - Cerebellum
Total median expression: 0.28 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Descriptions from all associated GenBank mRNAs
  DI443572 - KR 1020140074997-A/193: MICRORNAS IN NEURODEGENERATIVE DISORDERS.
DM176541 - A nucleics Acid regulating cell growth.
HW807898 - JP 2014534810-A/193: MICRORNAS IN NEURODEGENERATIVE DISORDERS.
HZ408442 - JP 2015528002-A/1039: CHIRAL CONTROL.
LG051776 - KR 1020150036642-A/1042: CHIRAL CONTROL.
DI443571 - KR 1020140074997-A/192: MICRORNAS IN NEURODEGENERATIVE DISORDERS.
DM176465 - A nucleics Acid regulating cell growth.
FW312195 - DETECTING NUCLEIC ACIDS.
FW364294 - Oligonucleotides for modulating target RNA activity.
HI965102 - Sequence 387 from Patent WO2010139812.
HI966410 - Sequence 730 from Patent WO2010139811.
HI967276 - Sequence 730 from Patent WO2010139810.
HV595203 - JP 2010539959-A/584: Micromirs.
HW807897 - JP 2014534810-A/192: MICRORNAS IN NEURODEGENERATIVE DISORDERS.
HZ074538 - JP 2013535982-A/629: Single-Stranded RNAi Agents Containing an Internal, Non-Nucleic Acid Spacer.
HZ082794 - JP 2015513906-A/151: Stem Cell Microparticles.
HZ157760 - JP 2015130862-A/584: Micromirs.
HZ186607 - JP 2015146814-A/356: Oligonucleotides for modulating target RNA activity.
HZ436575 - JP 2015529450-A/151: Stem Cell Microparticles.
HZ480458 - JP 2015535430-A/964: TERMINALLY MODIFIED RNA.
HZ790389 - JP 2016504050-A/2134: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
JA401140 - Sequence 387 from Patent WO2011080318.
JA402118 - Sequence 387 from Patent WO2011080315.
JA403125 - Sequence 387 from Patent WO2011080316.
JA405756 - Sequence 387 from Patent EP2336353.
JA427253 - Sequence 586 from Patent WO2011072177.
JA429282 - Sequence 387 from Patent WO2011095623.
JA791640 - Sequence 42 from Patent WO2012089772.
JB022517 - Sequence 359 from Patent WO2013026685.
JB023062 - Sequence 316 from Patent WO2013026684.
JB050134 - Sequence 221 from Patent WO2013020926.
JB074867 - Sequence 127 from Patent WO2012168448.
JB620723 - Sequence 703 from Patent WO2013063544.
JC171779 - Sequence 151 from Patent WO2013150303.
JC513121 - Sequence 3374 from Patent WO2014113089.
JC572590 - Sequence 151 from Patent WO2014125276.
JC582278 - Sequence 151 from Patent WO2014125277.
JE953974 - Sequence 151 from Patent WO2015052526.
JE955727 - Sequence 151 from Patent WO2015052527.
LF137479 - JP 2016507550-A/151: Method of Producing Microparticles.
LF161350 - JP 2016513950-A/1416: Oligomers with improved off-target profile.
LF162444 - JP 2016513095-A/151: Stem Cell Microparticles and miRNA.
LF953196 - JP 2016534103-A/151: Stem Cell Microparticles and miRNA.
LG005541 - KR 1020150004822-A/151: STEM CELL MICROPARTICLES.
LG053237 - KR 1020150059168-A/151: STEM CELL MICROPARTICLES.
LG240476 - KR 1020160035087-A/151: STEM CELL MICROPARTICLES AND MIRNA.
LP714647 - Sequence 19 from Patent WO2015092707.
LP715538 - Sequence 59 from Patent WO2016001030.
LP727036 - Sequence 730 from Patent EP2883966.
LP769515 - Sequence 42 from Patent EP3294910.
LP819306 - Sequence 730 from Patent EP3296406.
LP939248 - Sequence 730 from Patent EP3184651.
LP951888 - Sequence 394 from Patent WO2017157650.
LQ070709 - Sequence 1033 from Patent EP2964234.
LQ278582 - Sequence 121 from Patent WO2016066288.
LQ441120 - Sequence 127 from Patent EP3029157.
LV470561 - JP 2016534036-A/151: Stem Cell Microparticles and miRNA.
LY405268 - KR 1020160117535-A/573: POLY OLIGOMER COMPOUND WITH BIOCLEAVABLE CONJUGATES.
LY416923 - KR 1020160145093-A/29: USE OF MICRO-RIBONUCLEIC ACID(MIRNA) TO DIAGNOSE TRANSPLANT REJECTION AND TOLERANCE OF IMMUNOSUPPRESSION THERAPY.
LZ233964 - JP 2017184756-A/356: Oligonucleotides for modulating target RNA activity.
MA260507 - JP 2017505623-A/573: POLY OLIGOMER COMPOUND WITH BIOCLEAVABLE CONJUGATES.
MA400855 - JP 2018515097-A/42: Method for Diagnosis and Prognosis of Chronic Heart Failure.
MA405812 - WO 2018155427-A/586: A probe with reduced false positive binding, a method to desing the probe, and the use thereof.
MA644210 - JP 2017113010-A/2134: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
MA732094 - JP 2017140048-A/964: TERMINALLY MODIFIED RNA.
MP015318 - Sequence 521 from Patent WO2019016252.
MS673513 - Sequence 316 from Patent EP3135774.
MS834241 - Sequence 387 from Patent EP3112479.
MS901886 - Sequence 359 from Patent EP3156505.
MS930526 - Sequence 19 from Patent EP3178943.
MA801263 - JP 2018183181-A/2134: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
MP129251 - Sequence 151 from Patent EP3470073.
MP162174 - Sequence 151 from Patent WO2019069093.
MP395043 - Sequence 141 from Patent EP3565903.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000365696.1, ENST00000365696.2, ENST00000365696.3, NR_030398, uc022bzb.1, uc022bzb.2, uc022bzb.3
UCSC ID: ENST00000365696.4
RefSeq Accession: NR_030398

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.