Human Gene TMEM164 (ENST00000372073.5) from GENCODE V49
  Description: Positive regulator of ferroptosis. Selectively mediates ATG5- dependent autophagosome formation during ferroptosis, rather than during starvation, and regulates the degradation of ferritin, GPX4 and lipid droplets to increase iron accumulation and lipid peroxidation, thereby promoting ferroptotic cell death. (from UniProt Q5U3C3)
Gencode Transcript: ENST00000372073.5
Gencode Gene: ENSG00000157600.14
Transcript (Including UTRs)
   Position: hg38 chrX:110,003,095-110,177,788 Size: 174,694 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chrX:110,003,775-110,173,451 Size: 169,677 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2025-09-17 13:57:07

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:110,003,095-110,177,788)mRNA (may differ from genome)Protein (297 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
LynxMalacardsMGImyGene2PubMedUniProtKB

-  Comments and Description Text from UniProtKB
  ID: TM164_HUMAN
DESCRIPTION: RecName: Full=Transmembrane protein 164;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the TMEM164 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.09 RPKM in Colon - Transverse
Total median expression: 278.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -137.10336-0.408 Picture PostScript Text
3' UTR -1534.104337-0.354 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026508 - TMEM164

ModBase Predicted Comparative 3D Structure on Q5U3C3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Cellular Component:
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LF211687 - JP 2014500723-A/19190: Polycomb-Associated Non-Coding RNAs.
MA447264 - JP 2018138019-A/19190: Polycomb-Associated Non-Coding RNAs.
AK094127 - Homo sapiens cDNA FLJ36808 fis, clone ASTRO2000533.
AK026332 - Homo sapiens cDNA: FLJ22679 fis, clone HSI10687.
BC085605 - Homo sapiens transmembrane protein 164, mRNA (cDNA clone MGC:105056 IMAGE:6142249), complete cds.
BC111767 - Homo sapiens cDNA clone IMAGE:40026091.
KJ894946 - Synthetic construct Homo sapiens clone ccsbBroadEn_04340 TMEM164 gene, encodes complete protein.
JD054031 - Sequence 35055 from Patent EP1572962.
JD214371 - Sequence 195395 from Patent EP1572962.
JD059917 - Sequence 40941 from Patent EP1572962.
LF382489 - JP 2014500723-A/189992: Polycomb-Associated Non-Coding RNAs.
MA618066 - JP 2018138019-A/189992: Polycomb-Associated Non-Coding RNAs.
LF382498 - JP 2014500723-A/190001: Polycomb-Associated Non-Coding RNAs.
MA618075 - JP 2018138019-A/190001: Polycomb-Associated Non-Coding RNAs.
JD468090 - Sequence 449114 from Patent EP1572962.
JD554122 - Sequence 535146 from Patent EP1572962.
JD181689 - Sequence 162713 from Patent EP1572962.
JD281209 - Sequence 262233 from Patent EP1572962.
JD291176 - Sequence 272200 from Patent EP1572962.
JD164294 - Sequence 145318 from Patent EP1572962.
JD140903 - Sequence 121927 from Patent EP1572962.
LF382499 - JP 2014500723-A/190002: Polycomb-Associated Non-Coding RNAs.
MA618076 - JP 2018138019-A/190002: Polycomb-Associated Non-Coding RNAs.
LF382500 - JP 2014500723-A/190003: Polycomb-Associated Non-Coding RNAs.
MA618077 - JP 2018138019-A/190003: Polycomb-Associated Non-Coding RNAs.
LF382501 - JP 2014500723-A/190004: Polycomb-Associated Non-Coding RNAs.
MA618078 - JP 2018138019-A/190004: Polycomb-Associated Non-Coding RNAs.
AK057084 - Homo sapiens cDNA FLJ32522 fis, clone SMINT2000018.
LF382502 - JP 2014500723-A/190005: Polycomb-Associated Non-Coding RNAs.
MA618079 - JP 2018138019-A/190005: Polycomb-Associated Non-Coding RNAs.
LF382503 - JP 2014500723-A/190006: Polycomb-Associated Non-Coding RNAs.
MA618080 - JP 2018138019-A/190006: Polycomb-Associated Non-Coding RNAs.
BC071977 - Homo sapiens cDNA clone IMAGE:6651537, partial cds.
LF382504 - JP 2014500723-A/190007: Polycomb-Associated Non-Coding RNAs.
MA618081 - JP 2018138019-A/190007: Polycomb-Associated Non-Coding RNAs.
BC002716 - Homo sapiens hypothetical protein FLJ22679, mRNA (cDNA clone IMAGE:3631142), partial cds.
BC006360 - Homo sapiens mRNA similar to heparan sulfate (glucosamine) 3-O-sulfotransferase 2 (cDNA clone IMAGE:4126274).
AK000180 - Homo sapiens cDNA FLJ20173 fis, clone COL09814.
LF382505 - JP 2014500723-A/190008: Polycomb-Associated Non-Coding RNAs.
MA618082 - JP 2018138019-A/190008: Polycomb-Associated Non-Coding RNAs.
BC019032 - Homo sapiens cDNA clone IMAGE:4577686, **** WARNING: chimeric clone ****.
JD506984 - Sequence 488008 from Patent EP1572962.
JD172393 - Sequence 153417 from Patent EP1572962.
JD042159 - Sequence 23183 from Patent EP1572962.
JD498399 - Sequence 479423 from Patent EP1572962.
JD295787 - Sequence 276811 from Patent EP1572962.
JD543069 - Sequence 524093 from Patent EP1572962.
JD333502 - Sequence 314526 from Patent EP1572962.
JD153189 - Sequence 134213 from Patent EP1572962.
JD283866 - Sequence 264890 from Patent EP1572962.
JD357296 - Sequence 338320 from Patent EP1572962.
JD077917 - Sequence 58941 from Patent EP1572962.
JD025042 - Sequence 6066 from Patent EP1572962.
JD219287 - Sequence 200311 from Patent EP1572962.
JD032073 - Sequence 13097 from Patent EP1572962.
JD274464 - Sequence 255488 from Patent EP1572962.
JD022609 - Sequence 3633 from Patent EP1572962.
JD557101 - Sequence 538125 from Patent EP1572962.
JD133051 - Sequence 114075 from Patent EP1572962.
JD392331 - Sequence 373355 from Patent EP1572962.
JD390297 - Sequence 371321 from Patent EP1572962.
JD402426 - Sequence 383450 from Patent EP1572962.
JD163937 - Sequence 144961 from Patent EP1572962.
JD521354 - Sequence 502378 from Patent EP1572962.
JD492343 - Sequence 473367 from Patent EP1572962.
JD342486 - Sequence 323510 from Patent EP1572962.
JD162387 - Sequence 143411 from Patent EP1572962.
JD109785 - Sequence 90809 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AK026332, B3KSQ8, ENST00000372073.1, ENST00000372073.2, ENST00000372073.3, ENST00000372073.4, F5H2P2, Q5U3C3, TM164_HUMAN, TMEM164 , uc065arn.1
UCSC ID: ENST00000372073.5
RefSeq Accession: XM_005262205.5
Protein: Q5U3C3 (aka TM164_HUMAN)
CCDS: CCDS14550.2

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.