ID:GRD2I_MOUSE DESCRIPTION: RecName: Full=Delphilin; AltName: Full=Glutamate receptor, ionotropic, delta 2-interacting protein 1; FUNCTION: Postsynaptic scaffolding protein at the Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules. SUBUNIT: Interacts with C-terminus of the glutamate receptor GRID2 via PDZ domain. Isoform 2 interacts also with Profilin-2/PFN2 and with the monocarboxylate transporter SLC16A7 via PDZ domain. The interaction of isoform 2 with GRID2 is dependent on GRID2 phosphorylation by PKA. SUBCELLULAR LOCATION: Isoform 2: Cell junction, synapse, postsynaptic cell membrane. Cell projection, dendritic spine. Note=Localized to the postsynaptic junction site of the parallel fiber-Purkinje cell synapse. Highly present in the dendritic spines. SUBCELLULAR LOCATION: Isoform 3: Cell junction, synapse. Cell projection, dendritic spine. Cell membrane; Lipid-anchor (Potential). Note=Localized at the dendritic spines, but also in dendritic shafts apart fron spines. TISSUE SPECIFICITY: Isoform 1 is expressed in the cerebellum, but not in the cerebral cortex. Isoform 2 is expressed in the cell body of purkinge cells of the cerebellum and weakly expressed in the cerebrum and the brainstem as well as various nuclei of the thalamus. Isoform 2 is highly expressed in the cerebral cortex than in the cerebellum. Isoform 3 is expressed in the cerebellum and cerebrum. DEVELOPMENTAL STAGE: Isoform 3 expression is maintained throughout cerebellar development, while isoform 2 expression gradually decrease following the first postnatal week. DOMAIN: PDZ 1 domain is responsible for cytoplasmic clustering of isoform 1. PTM: Isoform 2 is palmitoylated. Palmitoylation of isoform 2 is necessary for the enhanced cell surface expression of GRID2, and is also responsible for the accumulation of isoform 2 within dendritic spines. Isoform 1 and isoform 2 are differentially localized, probably modulating GRID2 signaling in neurons. MISCELLANEOUS: Isoform 1 shows a punctate distribution throughout the cytoplasm when expressed in COS cells, whereas isoform 2 is enriched at the edges of the plasma membranes. When expressed in cultured hippocampal neurons, isoform 1 forms clusters mainly in the dendritic shafts, whereas isoform 2 is preferentially expressed in spines. SIMILARITY: Contains 1 FH2 (formin homology 2) domain. SIMILARITY: Contains 2 PDZ (DHR) domains.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF02181 - Formin Homology 2 Domain PF00595 - PDZ domain (Also known as DHR or GLGF)
ModBase Predicted Comparative 3D Structure on Q0QWG9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.