Mouse Gene Mkrn1 (ENSMUST00000114822.1) Description and Page Index
  Description: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase (By similarity). Has negative and positive effects on RNA polymerase II-dependent transcription. (from UniProt Q9QXP6)
Gencode Transcript: ENSMUST00000114822.1
Gencode Gene: ENSMUSG00000029922.15
Transcript (Including UTRs)
   Position: mm10 chr6:39,405,539-39,420,298 Size: 14,760 Total Exon Count: 3 Strand: -
Coding Region
   Position: mm10 chr6:39,405,684-39,420,283 Size: 14,600 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:39,405,539-39,420,298)mRNA (may differ from genome)Protein (196 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaBioGPS
CGAPEnsemblExonPrimerGeneCardsMGIPubMed
ReactomeStanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: MKRN1_MOUSE
DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase makorin-1; EC=6.3.2.-;
FUNCTION: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase (By similarity). Has negative and positive effects on RNA polymerase II-dependent transcription.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with p53/TP53 and CDKN1A. Interacts with TERT, modulating telomere length homeostasis (By similarity).
TISSUE SPECIFICITY: Highly expressed in embryo, in specific cell types of the central nervous system, in brain with the strongest levels of expression in the mantle layers and in testis. Moderate to low levels in somatic tissues.
DEVELOPMENTAL STAGE: Not detected until 11 dpc. Restricted to developing central nervous system in 13 days embryos.
PTM: Auto-ubiquitinated; which leads to proteasomal degradation (By similarity).
DISRUPTION PHENOTYPE: PubMed:12968785 describes 80 % of lethality within 2 days of birth in heterozygotes and polycystic kidney and bone deformity in survivors. However, PubMed:16882727 reports no apparent developmental deficits with viable and fertile mice.
SIMILARITY: Contains 4 C3H1-type zinc fingers.
SIMILARITY: Contains 1 RING-type zinc finger.
CAUTION: PubMed:12968785 shows that the expression of the transcribed processed pseudogenes (TPP) Makorin1-p1 prevents the decay of functional Mkrn1 mRNAs. In contrast, PubMed:16882727 shows that Makorin1-p1 is not transcribed and that the putative transcripts represent an alternative isoform of the Mkrn1 source gene.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -22.00145-0.152 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026290 - Makorin-related
IPR000571 - Znf_CCCH
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD
IPR017907 - Znf_RING_CS

Pfam Domains:
PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar)
PF14608 - RNA-binding, Nab2-type zinc finger

SCOP Domains:
90229 - CCCH zinc finger

ModBase Predicted Comparative 3D Structure on Q9QXP6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003682 chromatin binding
GO:0004842 ubiquitin-protein transferase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0016567 protein ubiquitination
GO:1990830 cellular response to leukemia inhibitory factor


-  Descriptions from all associated GenBank mRNAs
  AF192785 - Mus musculus makorin 1 (Mkrn1) mRNA, complete cds.
AK077060 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4932442G21 product:makorin, ring finger protein, 1, full insert sequence.
AK029500 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4921508B10 product:makorin, ring finger protein, 1, full insert sequence.
AB026986 - Mus musculus mRNA for RFP, complete cds.
BC003329 - Mus musculus makorin, ring finger protein, 1, mRNA (cDNA clone MGC:7132 IMAGE:3158135), complete cds.
AK005137 - Mus musculus adult male cerebellum cDNA, RIKEN full-length enriched library, clone:1500003O18 product:makorin, ring finger protein, 1, full insert sequence.
AK215701 - Mus musculus cDNA, clone:Y2G0135O09, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031985, based on BLAT search.
AK210725 - Mus musculus cDNA, clone:Y2G0119G04, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031985, based on BLAT search.
AK186489 - Mus musculus cDNA, clone:Y0G0137I04, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031985, based on BLAT search.
AK207305 - Mus musculus cDNA, clone:Y2G0107J15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031985, based on BLAT search.
AK078962 - Mus musculus adult male cecum cDNA, RIKEN full-length enriched library, clone:9130412D24 product:makorin, ring finger protein, 1, full insert sequence.
LF237240 - JP 2014500723-A/44743: Polycomb-Associated Non-Coding RNAs.
AK188467 - Mus musculus cDNA, clone:Y0G0145F12, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031985, based on BLAT search.
MA472817 - JP 2018138019-A/44743: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9QXP6 (Reactome details) participates in the following event(s):

R-MMU-8948757 AKT phosphorylates MKRN1
R-MMU-983157 Interaction of E3 with substrate and E2-Ub complex
R-MMU-983147 Release of E3 from polyubiquitinated substrate
R-MMU-8948775 MKRN1 polyubiquitinates PTEN
R-MMU-983140 Transfer of Ub from E2 to substrate and release of E2
R-MMU-198323 AKT phosphorylates targets in the cytosol
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-6807070 PTEN Regulation
R-MMU-983169 Class I MHC mediated antigen processing & presentation
R-MMU-9006925 Intracellular signaling by second messengers
R-MMU-1280218 Adaptive Immune System
R-MMU-162582 Signal Transduction
R-MMU-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: AK078962, MKRN1_MOUSE, Q9QXP6, uc009blu.1, uc009blu.2
UCSC ID: uc009blu.2
RefSeq Accession: NM_018810
Protein: Q9QXP6 (aka MKRN1_MOUSE or MKR1_MOUSE)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.