Mouse Gene Mkrn1 (ENSMUST00000114822.1) Description and Page Index
Description: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase (By similarity). Has negative and positive effects on RNA polymerase II-dependent transcription. (from UniProt Q9QXP6) Gencode Transcript: ENSMUST00000114822.1 Gencode Gene: ENSMUSG00000029922.15 Transcript (Including UTRs) Position: mm10 chr6:39,405,539-39,420,298 Size: 14,760 Total Exon Count: 3 Strand: - Coding Region Position: mm10 chr6:39,405,684-39,420,283 Size: 14,600 Coding Exon Count: 3
ID:MKRN1_MOUSE DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase makorin-1; EC=6.3.2.-; FUNCTION: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase (By similarity). Has negative and positive effects on RNA polymerase II-dependent transcription. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with p53/TP53 and CDKN1A. Interacts with TERT, modulating telomere length homeostasis (By similarity). TISSUE SPECIFICITY: Highly expressed in embryo, in specific cell types of the central nervous system, in brain with the strongest levels of expression in the mantle layers and in testis. Moderate to low levels in somatic tissues. DEVELOPMENTAL STAGE: Not detected until 11 dpc. Restricted to developing central nervous system in 13 days embryos. PTM: Auto-ubiquitinated; which leads to proteasomal degradation (By similarity). DISRUPTION PHENOTYPE: PubMed:12968785 describes 80 % of lethality within 2 days of birth in heterozygotes and polycystic kidney and bone deformity in survivors. However, PubMed:16882727 reports no apparent developmental deficits with viable and fertile mice. SIMILARITY: Contains 4 C3H1-type zinc fingers. SIMILARITY: Contains 1 RING-type zinc finger. CAUTION: PubMed:12968785 shows that the expression of the transcribed processed pseudogenes (TPP) Makorin1-p1 prevents the decay of functional Mkrn1 mRNAs. In contrast, PubMed:16882727 shows that Makorin1-p1 is not transcribed and that the putative transcripts represent an alternative isoform of the Mkrn1 source gene.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9QXP6
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.