Human Gene BRF2 (ENST00000220659.11) Description and Page Index
  Description: Homo sapiens BRF2 RNA polymerase III transcription initiation factor subunit (BRF2), mRNA. (from RefSeq NM_018310)
RefSeq Summary (NM_018310): This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.427703.1, DRR138522.14564.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000220659.11/ ENSP00000220659.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Gencode Transcript: ENST00000220659.11
Gencode Gene: ENSG00000104221.13
Transcript (Including UTRs)
   Position: hg38 chr8:37,843,268-37,849,861 Size: 6,594 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr8:37,844,490-37,849,783 Size: 5,294 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr8:37,843,268-37,849,861)mRNA (may differ from genome)Protein (419 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
ReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Transcription factor IIIB 50 kDa subunit; Short=TFIIIB50; Short=hTFIIIB50; AltName: Full=B-related factor 2; Short=BRF-2; AltName: Full=hBRFU;
FUNCTION: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBUNIT: Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
INDUCTION: Down-regulated by epigallocatechin gallate (EGCG) treatment.
SIMILARITY: Belongs to the TFIIB family.
SIMILARITY: Contains 1 TFIIB-type zinc finger.
SEQUENCE CAUTION: Sequence=AAG35486.1; Type=Erroneous initiation;

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.08 RPKM in Muscle - Skeletal
Total median expression: 221.99 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.1078-0.322 Picture PostScript Text
3' UTR -438.271222-0.359 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013763 - Cyclin-like
IPR000812 - TFIIB
IPR013137 - Znf_TFIIB

Pfam Domains:
PF08271 - TFIIB zinc-binding

ModBase Predicted Comparative 3D Structure on Q9HAW0
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001032 RNA polymerase III type 3 promoter DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006359 regulation of transcription from RNA polymerase III promoter
GO:0034599 cellular response to oxidative stress
GO:0070897 DNA-templated transcriptional preinitiation complex assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm

-  Descriptions from all associated GenBank mRNAs
  JD019532 - Sequence 556 from Patent EP1572962.
JD027720 - Sequence 8744 from Patent EP1572962.
JD021211 - Sequence 2235 from Patent EP1572962.
JD031609 - Sequence 12633 from Patent EP1572962.
JD021198 - Sequence 2222 from Patent EP1572962.
AF130058 - Homo sapiens clone FLB5619 PRO1470 mRNA, complete cds.
BC010648 - Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, mRNA (cDNA clone MGC:9916 IMAGE:3871326), complete cds.
AF206673 - Homo sapiens TFIIIB50 mRNA, complete cds.
AK001914 - Homo sapiens cDNA FLJ11052 fis, clone PLACE1004645.
JD033972 - Sequence 14996 from Patent EP1572962.
JD448036 - Sequence 429060 from Patent EP1572962.
JD243962 - Sequence 224986 from Patent EP1572962.
JD226354 - Sequence 207378 from Patent EP1572962.
JD178252 - Sequence 159276 from Patent EP1572962.
JD255036 - Sequence 236060 from Patent EP1572962.
JD060226 - Sequence 41250 from Patent EP1572962.
JD162178 - Sequence 143202 from Patent EP1572962.
JD450442 - Sequence 431466 from Patent EP1572962.
JD043902 - Sequence 24926 from Patent EP1572962.
JD053631 - Sequence 34655 from Patent EP1572962.
AF298153 - Homo sapiens RNA polymerase III transcription initiation factor BRFU mRNA, complete cds.
AK294337 - Homo sapiens cDNA FLJ51823 complete cds, highly similar to Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mRNA.
AK315420 - Homo sapiens cDNA, FLJ96474, Homo sapiens BRF2, subunit of RNA polymerase III transcriptioninitiation factor, BRF1-like (BRF2), mRNA.
KJ894179 - Synthetic construct Homo sapiens clone ccsbBroadEn_03573 BRF2 gene, encodes complete protein.
EU446514 - Synthetic construct Homo sapiens clone IMAGE:100069872; IMAGE:100011723; FLH258608.01L BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) gene, encodes complete protein.
AB463449 - Synthetic construct DNA, clone: pF1KB7707, Homo sapiens BRF2 gene for BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, without stop codon, in Flexi system.
CU679783 - Synthetic construct Homo sapiens gateway clone IMAGE:100017226 5' read BRF2 mRNA.
JD234433 - Sequence 215457 from Patent EP1572962.
JD547205 - Sequence 528229 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9HAW0 (Reactome details) participates in the following event(s):

R-HSA-83793 Binding of TFIIIB to SNAPc:Oct-1:Staf:Type 3 Promoter Complex
R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex
R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters
R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters
R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76046 RNA Polymerase III Transcription Initiation
R-HSA-74158 RNA Polymerase III Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: B2RD62, BRF2_HUMAN, BRFU, D3DSW6, NM_018310, PRO1470, Q9H2Y3, Q9H3B3, Q9HAW0, Q9NUY6, uc003xkk.1, uc003xkk.2, uc003xkk.3, uc003xkk.4, uc003xkk.5
UCSC ID: uc003xkk.5
RefSeq Accession: NM_018310
Protein: Q9HAW0 (aka BRF2_HUMAN)
CCDS: CCDS6098.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.