Human Gene PGA5 (ENST00000312403.9) Description and Page Index
  Description: Homo sapiens pepsinogen A5 (PGA5), mRNA. (from RefSeq NM_014224)
RefSeq Summary (NM_014224): This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029055.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Gencode Transcript: ENST00000312403.9
Gencode Gene: ENSG00000256713.7
Transcript (Including UTRs)
   Position: hg38 chr11:61,241,042-61,251,448 Size: 10,407 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg38 chr11:61,241,227-61,251,281 Size: 10,055 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:61,241,042-61,251,448)mRNA (may differ from genome)Protein (388 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
HPRDLynxMGIneXtProtOMIMPubMed
ReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: PEPA5_HUMAN
DESCRIPTION: RecName: Full=Pepsin A-5; EC=3.4.23.1; AltName: Full=Pepsinogen-5; Flags: Precursor;
FUNCTION: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
CATALYTIC ACTIVITY: Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|- Tyr-26 bonds in the B chain of insulin.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the peptidase A1 family.

-  MalaCards Disease Associations
  MalaCards Gene Search: PGA5
Diseases sorted by gene-association score: atrophic gastritis (7), gastritis (6), amyotrophic lateral sclerosis 1 (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1757.52 RPKM in Stomach
Total median expression: 1758.13 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -63.30185-0.342 Picture PostScript Text
3' UTR -35.90167-0.215 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001461 - Peptidase_A1
IPR021109 - Peptidase_aspartic
IPR001969 - Peptidase_aspartic_AS
IPR009007 - Peptidase_aspartic_catalytic
IPR012848 - Propep_A1

Pfam Domains:
PF07966 - A1 Propeptide
PF00026 - Eukaryotic aspartyl protease

ModBase Predicted Comparative 3D Structure on P0DJD9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI  Ensembl  
Protein Sequence  Protein Sequence  
Alignment  Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004190 aspartic-type endopeptidase activity
GO:0008233 peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0006914 autophagy
GO:0007586 digestion
GO:0030163 protein catabolic process
GO:0044267 cellular protein metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0070062 extracellular exosome
GO:0097486 multivesicular body lumen


-  Descriptions from all associated GenBank mRNAs
  BC171889 - Homo sapiens pepsinogen 5, group I (pepsinogen A), mRNA (cDNA clone MGC:198604 IMAGE:9054543), complete cds.
BC146999 - Homo sapiens pepsinogen 5, group I (pepsinogen A), mRNA (cDNA clone MGC:182102 IMAGE:9056927), complete cds.
BC171897 - Homo sapiens pepsinogen 5, group I (pepsinogen A), mRNA (cDNA clone MGC:198612 IMAGE:9054551), complete cds.
BC029055 - Homo sapiens pepsinogen 5, group I (pepsinogen A), mRNA (cDNA clone MGC:36750 IMAGE:5184316), complete cds.
JD194045 - Sequence 175069 from Patent EP1572962.
JD450690 - Sequence 431714 from Patent EP1572962.
JD023172 - Sequence 4196 from Patent EP1572962.
JD035985 - Sequence 17009 from Patent EP1572962.
JD020735 - Sequence 1759 from Patent EP1572962.
JD033527 - Sequence 14551 from Patent EP1572962.
JD035979 - Sequence 17003 from Patent EP1572962.
JD182965 - Sequence 163989 from Patent EP1572962.
JD411014 - Sequence 392038 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P0DJD9 (Reactome details) participates in the following event(s):

R-HSA-5684864 NAPSA, CTSH, PGA3-5 cleave pro-SFTPB
R-HSA-5685902 NAPSA, CTSH, PGA3-5 cleave pro-SFTPC
R-HSA-5683826 Surfactant metabolism
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A8K749, B7ZW62, B7ZW75, NM_014224, P00790, P0DJD9, PEPA5_HUMAN, Q7M4R0, Q8N1E3, uc001nqz.1, uc001nqz.2, uc001nqz.3, uc001nqz.4
UCSC ID: uc001nqz.4
RefSeq Accession: NM_014224
Protein: P0DJD9 (aka PEPA5_HUMAN)
CCDS: CCDS8001.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.