Human Gene KLHL21 (ENST00000377658.8) from GENCODE V44
  Description: Homo sapiens kelch like family member 21 (KLHL21), transcript variant 1, mRNA. (from RefSeq NM_014851)
Gencode Transcript: ENST00000377658.8
Gencode Gene: ENSG00000162413.17
Transcript (Including UTRs)
   Position: hg38 chr1:6,590,724-6,602,869 Size: 12,146 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr1:6,593,365-6,602,817 Size: 9,453 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr1:6,590,724-6,602,869)mRNA (may differ from genome)Protein (597 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Kelch-like protein 21;
FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle. Note=Localizes to the spindle midzone and targets CUL3 to this region.
SIMILARITY: Contains 1 BACK (BTB/Kelch associated) domain.
SIMILARITY: Contains 1 BTB (POZ) domain.
SIMILARITY: Contains 6 Kelch repeats.
SEQUENCE CAUTION: Sequence=BAA32314.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 41.41 RPKM in Muscle - Skeletal
Total median expression: 791.78 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.1052-0.367 Picture PostScript Text
3' UTR -1090.902641-0.413 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011705 - BACK
IPR000210 - BTB/POZ-like
IPR011333 - BTB/POZ_fold
IPR013069 - BTB_POZ
IPR015916 - Gal_Oxidase_b-propeller
IPR017096 - Kelch-like_gigaxonin
IPR006652 - Kelch_1

Pfam Domains:
PF07707 - BTB And C-terminal Kelch
PF00651 - BTB/POZ domain
PF01344 - Kelch motif

ModBase Predicted Comparative 3D Structure on Q9UJP4
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein SequenceProtein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity

Biological Process:
GO:0007049 cell cycle
GO:0016567 protein ubiquitination
GO:0032465 regulation of cytokinesis
GO:0035853 chromosome passenger complex localization to spindle midzone
GO:0043687 post-translational protein modification
GO:0051301 cell division

Cellular Component:
GO:0005737 cytoplasm
GO:0005819 spindle
GO:0005827 polar microtubule
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0031463 Cul3-RING ubiquitin ligase complex

-  Descriptions from all associated GenBank mRNAs
  AK075305 - Homo sapiens cDNA FLJ90824 fis, clone Y79AA1001430, highly similar to Homo sapiens kelch-like 21 (Drosophila) (KLHL21), mRNA.
AB007938 - Homo sapiens mRNA for KIAA0469 protein, partial cds.
AX810687 - Sequence 11 from Patent EP1333092.
BC064980 - Homo sapiens kelch-like 21 (Drosophila), mRNA (cDNA clone IMAGE:6577755).
BC091648 - Homo sapiens kelch-like 21 (Drosophila), mRNA (cDNA clone MGC:99635 IMAGE:6373903), complete cds.
BC034039 - Homo sapiens kelch-like 21 (Drosophila), mRNA (cDNA clone MGC:26467 IMAGE:4811534), complete cds.
AK057164 - Homo sapiens cDNA FLJ32602 fis, clone STOMA1000091.
AK090472 - Homo sapiens mRNA for FLJ00393 protein.
JD516769 - Sequence 497793 from Patent EP1572962.
JD342598 - Sequence 323622 from Patent EP1572962.
JD112597 - Sequence 93621 from Patent EP1572962.
JD549428 - Sequence 530452 from Patent EP1572962.
JD183059 - Sequence 164083 from Patent EP1572962.
JD555042 - Sequence 536066 from Patent EP1572962.
JD077838 - Sequence 58862 from Patent EP1572962.
JD384395 - Sequence 365419 from Patent EP1572962.
JD487616 - Sequence 468640 from Patent EP1572962.
JD243868 - Sequence 224892 from Patent EP1572962.
JD328953 - Sequence 309977 from Patent EP1572962.
JD547409 - Sequence 528433 from Patent EP1572962.
JD203356 - Sequence 184380 from Patent EP1572962.
JD187643 - Sequence 168667 from Patent EP1572962.
JD560436 - Sequence 541460 from Patent EP1572962.
JD279706 - Sequence 260730 from Patent EP1572962.
JD174506 - Sequence 155530 from Patent EP1572962.
JD330359 - Sequence 311383 from Patent EP1572962.
JD199305 - Sequence 180329 from Patent EP1572962.
JD135453 - Sequence 116477 from Patent EP1572962.
JD555775 - Sequence 536799 from Patent EP1572962.
JD203492 - Sequence 184516 from Patent EP1572962.
JD353805 - Sequence 334829 from Patent EP1572962.
JD161209 - Sequence 142233 from Patent EP1572962.
JD467301 - Sequence 448325 from Patent EP1572962.
JD273234 - Sequence 254258 from Patent EP1572962.
JD294726 - Sequence 275750 from Patent EP1572962.
JD201819 - Sequence 182843 from Patent EP1572962.
JD115183 - Sequence 96207 from Patent EP1572962.
JD159916 - Sequence 140940 from Patent EP1572962.
JD422877 - Sequence 403901 from Patent EP1572962.
JD318403 - Sequence 299427 from Patent EP1572962.
JD420018 - Sequence 401042 from Patent EP1572962.
JD372490 - Sequence 353514 from Patent EP1572962.
JD308366 - Sequence 289390 from Patent EP1572962.
JD195559 - Sequence 176583 from Patent EP1572962.
JD391117 - Sequence 372141 from Patent EP1572962.
DQ597226 - Homo sapiens piRNA piR-35292, complete sequence.
JD518733 - Sequence 499757 from Patent EP1572962.
DQ588480 - Homo sapiens piRNA piR-55592, complete sequence.
JD042802 - Sequence 23826 from Patent EP1572962.
JD506935 - Sequence 487959 from Patent EP1572962.
JD276631 - Sequence 257655 from Patent EP1572962.
JD222997 - Sequence 204021 from Patent EP1572962.
JD143837 - Sequence 124861 from Patent EP1572962.
JD163705 - Sequence 144729 from Patent EP1572962.
JD559078 - Sequence 540102 from Patent EP1572962.
JD174651 - Sequence 155675 from Patent EP1572962.
JD392927 - Sequence 373951 from Patent EP1572962.
JD487681 - Sequence 468705 from Patent EP1572962.
JD498597 - Sequence 479621 from Patent EP1572962.
JD109158 - Sequence 90182 from Patent EP1572962.
JD213896 - Sequence 194920 from Patent EP1572962.
JD516909 - Sequence 497933 from Patent EP1572962.
JD248318 - Sequence 229342 from Patent EP1572962.
JD519268 - Sequence 500292 from Patent EP1572962.
JD130675 - Sequence 111699 from Patent EP1572962.
JD278779 - Sequence 259803 from Patent EP1572962.
JD106405 - Sequence 87429 from Patent EP1572962.
JD321304 - Sequence 302328 from Patent EP1572962.
BC111766 - Homo sapiens kelch-like 21 (Drosophila), mRNA (cDNA clone IMAGE:40026322), complete cds.
JD198763 - Sequence 179787 from Patent EP1572962.
JD178194 - Sequence 159218 from Patent EP1572962.
AK307564 - Homo sapiens cDNA, FLJ97512.
JD538246 - Sequence 519270 from Patent EP1572962.
JD158525 - Sequence 139549 from Patent EP1572962.
JD159293 - Sequence 140317 from Patent EP1572962.
JD199902 - Sequence 180926 from Patent EP1572962.
JD532709 - Sequence 513733 from Patent EP1572962.
JD520327 - Sequence 501351 from Patent EP1572962.
JD340862 - Sequence 321886 from Patent EP1572962.
DQ586011 - Homo sapiens piRNA piR-53123, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UJP4 (Reactome details) participates in the following event(s):

R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-597592 Post-translational protein modification
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-392499 Metabolism of proteins
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B3KQP2, ENST00000377658.1, ENST00000377658.2, ENST00000377658.3, ENST00000377658.4, ENST00000377658.5, ENST00000377658.6, ENST00000377658.7, KIAA0469, KLH21_HUMAN, NM_014851, O75057, Q5SY26, Q5SY28, Q8N4I6, Q8NF10, Q9UJP4, uc001aoa.1, uc001aoa.2, uc001aoa.3
UCSC ID: ENST00000377658.8
RefSeq Accession: NM_014851
Protein: Q9UJP4 (aka KLH21_HUMAN or Y469_HUMAN)
CCDS: CCDS30575.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.