Human Gene CRY2 (ENST00000417225.6) from GENCODE V38
Description: Homo sapiens cryptochrome circadian regulator 2 (CRY2), transcript variant 2, mRNA. (from RefSeq NM_001127457) RefSeq Summary (NM_001127457): This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]. Gencode Transcript: ENST00000417225.6 Gencode Gene: ENSG00000121671.12 Transcript (Including UTRs) Position: hg38 chr11:45,847,118-45,881,233 Size: 34,116 Total Exon Count: 12 Strand: + Coding Region Position: hg38 chr11:45,847,250-45,872,231 Size: 24,982 Coding Exon Count: 11
ID:CRY2_HUMAN DESCRIPTION: RecName: Full=Cryptochrome-2; FUNCTION: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. COFACTOR: Binds 1 FAD per subunit (By similarity). COFACTOR: Binds 1 5,10-methenyltetrahydrofolate non-covalently per subunit (By similarity). SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Interacts with NFIL3. Interacts with FBXL3. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL. TISSUE SPECIFICITY: Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. PTM: Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2. PTM: Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation. SIMILARITY: Belongs to the DNA photolyase class-1 family. SIMILARITY: Contains 1 photolyase/cryptochrome alpha/beta domain. SEQUENCE CAUTION: Sequence=AAH35161.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. Aberrant splice sites; Sequence=BAG57993.1; Type=Erroneous termination; Positions=110; Note=Translated as Trp; Sequence=BAG57993.1; Type=Erroneous translation; Note=Wrong choice of CDS; WEB RESOURCE: Name=Wikipedia; Note=Cryptochrome entry; URL="http://en.wikipedia.org/wiki/Cryptochrome";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00875 - DNA photolyase PF03441 - FAD binding domain of DNA photolyase
ModBase Predicted Comparative 3D Structure on Q49AN0
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.