Human Gene EIF4G3 (ENST00000602326.5) from GENCODE V38
  Description: Homo sapiens eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant 2, mRNA. (from RefSeq NM_001198802)
RefSeq Summary (NM_001198802): The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016].
Gencode Transcript: ENST00000602326.5
Gencode Gene: ENSG00000075151.23
Transcript (Including UTRs)
   Position: hg38 chr1:20,806,483-21,176,850 Size: 370,368 Total Exon Count: 35 Strand: -
Coding Region
   Position: hg38 chr1:20,807,319-21,002,742 Size: 195,424 Coding Exon Count: 31 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2021-09-27 09:51:20

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr1:20,806,483-21,176,850)mRNA (may differ from genome)Protein (1591 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 4 gamma 3; Short=eIF-4-gamma 3; Short=eIF-4G 3; Short=eIF4G 3; AltName: Full=eIF-4-gamma II; Short=eIF4GII;
FUNCTION: Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
SUBUNIT: Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (By similarity).
PTM: Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
SIMILARITY: Belongs to the eIF4G family.
SIMILARITY: Contains 5 HEAT repeats.
SIMILARITY: Contains 1 MI domain.
SIMILARITY: Contains 1 MIF4G domain.
SIMILARITY: Contains 1 W2 domain.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 44.17 RPKM in Testis
Total median expression: 592.78 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -210.40584-0.360 Picture PostScript Text
3' UTR -151.70836-0.181 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016024 - ARM-type_fold
IPR003891 - Initiation_fac_eIF4g_MI
IPR016021 - MIF4-like_typ_1/2/3
IPR003890 - MIF4G-like_typ-3
IPR003307 - W2_domain

Pfam Domains:
PF02847 - MA3 domain
PF02854 - MIF4G domain
PF02020 - eIF4-gamma/eIF5/eIF2-epsilon

Protein Data Bank (PDB) 3-D Structure
MuPIT help

- X-ray MuPIT

ModBase Predicted Comparative 3D Structure on O43432
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000339 RNA cap binding
GO:0003723 RNA binding
GO:0003743 translation initiation factor activity
GO:0005515 protein binding
GO:0008135 translation factor activity, RNA binding

Biological Process:
GO:0006412 translation
GO:0006413 translational initiation
GO:0006417 regulation of translation
GO:0006446 regulation of translational initiation
GO:0016032 viral process
GO:0010507 negative regulation of autophagy

Cellular Component:
GO:0005829 cytosol
GO:0016281 eukaryotic translation initiation factor 4F complex

-  Descriptions from all associated GenBank mRNAs
  AF012072 - Homo sapiens eIF4GII mRNA, complete cds.
AB209119 - Homo sapiens mRNA for eukaryotic translation initiation factor 4 gamma, 3 variant protein.
BC094683 - Homo sapiens eukaryotic translation initiation factor 4 gamma, 3, mRNA (cDNA clone MGC:102792 IMAGE:6188273), complete cds.
BC072413 - Homo sapiens eukaryotic translation initiation factor 4 gamma, 3, mRNA (cDNA clone IMAGE:6165611), partial cds.
BC136643 - Homo sapiens eukaryotic translation initiation factor 4 gamma, 3, mRNA (cDNA clone MGC:168256 IMAGE:9020633), complete cds.
BC144335 - Homo sapiens cDNA clone IMAGE:9052855, containing frame-shift errors.
AK294883 - Homo sapiens cDNA FLJ59298 complete cds, highly similar to Eukaryotic translation initiation factor 4 gamma 3.
AK225489 - Homo sapiens mRNA for Splice isoform 2 of O43432 variant, clone: JTH03575.
BC030578 - Homo sapiens eukaryotic translation initiation factor 4 gamma, 3, mRNA (cDNA clone IMAGE:4836870), complete cds.
AK302087 - Homo sapiens cDNA FLJ60945 complete cds, highly similar to Eukaryotic translation initiation factor 4gamma 3.
AK302873 - Homo sapiens cDNA FLJ53944 complete cds, highly similar to Eukaryotic translation initiation factor 4 gamma 3.
Z34918 - H.sapiens mRNA for translation initiation factor eIF-4gamma (partial).
JD558073 - Sequence 539097 from Patent EP1572962.
JD261059 - Sequence 242083 from Patent EP1572962.
JD164334 - Sequence 145358 from Patent EP1572962.
JD102602 - Sequence 83626 from Patent EP1572962.
AK310195 - Homo sapiens cDNA, FLJ17237.
BX647897 - Homo sapiens mRNA; cDNA DKFZp686C17112 (from clone DKFZp686C17112).
JD244587 - Sequence 225611 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa05416 - Viral myocarditis

BioCarta from NCI Cancer Genome Anatomy Project
h_eif4Pathway - Regulation of eIF4e and p70 S6 Kinase
h_eifPathway - Eukaryotic protein translation
h_mTORPathway - mTOR Signaling Pathway
h_iresPathway - Internal Ribosome entry pathway

Reactome (by CSHL, EBI, and GO)

Protein O43432 (Reactome details) participates in the following event(s):

R-HSA-1678842 Competitive inhibition of translation initiation by ISGylated 4EHP
R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-1169410 Antiviral mechanism by IFN-stimulated genes
R-HSA-913531 Interferon Signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B9EGQ7, ENST00000602326.1, ENST00000602326.2, ENST00000602326.3, ENST00000602326.4, IF4G3_HUMAN, NM_001198802, O43432, Q15597, Q5SWC3, Q8NEN1, uc001bee.1, uc001bee.2, uc001bee.3, uc001bee.4
UCSC ID: ENST00000602326.5
RefSeq Accession: NM_001198802
Protein: O43432 (aka IF4G3_HUMAN or I4G3_HUMAN)
CCDS: CCDS214.1, CCDS55580.1, CCDS72723.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.