S. cerevisiae Gene MGT1 (YDL200C)
  Description: DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage
Transcript (Including UTRs)
   Position: sacCer3 chrIV:100,501-101,067 Size: 567 Total Exon Count: 1 Strand: -
Coding Region
   Position: sacCer3 chrIV:100,501-101,067 Size: 567 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsProtein StructureOther SpeciesGO Annotations
Other NamesMethods
Data last updated at UCSC: 2011-08-29

-  Sequence and Links to Tools and Databases
Genomic Sequence (chrIV:100,501-101,067)mRNAProtein (188 aa)
Gene SorterGenome BrowserOther Species FASTASGDUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Methylated-DNA--protein-cysteine methyltransferase; EC=; AltName: Full=6-O-methylguanine-DNA methyltransferase; Short=MGMT; AltName: Full=DNA repair MTase; AltName: Full=O-6-methylguanine-DNA-alkyltransferase;
FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine. Prefers double-stranded DNA over single-stranded DNA as substrate.
CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L- cysteine.
INDUCTION: In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents.
MISCELLANEOUS: Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells.
MISCELLANEOUS: This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
SIMILARITY: Belongs to the MGMT family.
SEQUENCE CAUTION: Sequence=AAA34780.1; Type=Erroneous initiation; Sequence=CAA42920.1; Type=Erroneous initiation; Sequence=CAA67469.1; Type=Erroneous initiation; Sequence=CAA98777.1; Type=Erroneous initiation; Sequence=CAA98778.1; Type=Erroneous initiation;

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001497 - MethylDNA_cys_MeTrfase_AS
IPR014048 - MethylDNA_cys_MeTrfase_DNA-bd
IPR008332 - MethylG_MeTrfase
IPR011991 - WHTH_trsnscrt_rep_DNA-bd

Pfam Domains:
PF01035 - 6-O-methylguanine DNA methyltransferase, DNA binding domain
PF02870 - 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain

ModBase Predicted Comparative 3D Structure on P26188
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanMouseRatZebrafishD. melanogasterC. elegans
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