S. cerevisiae Gene MYO3 (YKL129C)
  Description: One of two type I myosins localizes to actin cortical patches deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
Transcript (Including UTRs)
   Position: sacCer3 chrXI:196,349-200,167 Size: 3,819 Total Exon Count: 1 Strand: -
Coding Region
   Position: sacCer3 chrXI:196,349-200,167 Size: 3,819 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2011-08-29

-  Sequence and Links to Tools and Databases
Genomic Sequence (chrXI:196,349-200,167)mRNAProtein (1272 aa)
Gene SorterGenome BrowserOther Species FASTASGDUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Myosin-3; AltName: Full=Actin-dependent myosin-I MYO3; AltName: Full=Class I unconventional myosin MYO3; AltName: Full=Type I myosin MYO3;
FUNCTION: One of two redundant type-I myosins implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization and for the internalization step in endocytosis. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions redundantly with LAS17 as actin nucleation-promoting factor (NPF) for the Arp2/3 complex. Motor domain phosphorylation by PAK kinases CLA4 and STE20 promotes CDC42-regulated actin assembly. Functions together with the NPF PAN1 in late stages of endocytosis. Motor domain phosphorylation by PDK1 kinases PKH1 and PKH2, and by SGK kinases YPK1 and YPK2, promotes ligand-induced, but not constitutive endocytosis of the G protein-coupled receptor STE2.
SUBUNIT: Interacts (via myosin head-like domain) with SHE4; this interaction is important for proper localization and may regulate the interaction of the motor domain with actin. Interacts (via SH3 domain) with VRP1; this interaction is required for localization to sites of polarized growth and may regulate the interaction of the tail domain with actin. Interacts (via SH3 domain) with PAN1; this interaction is important for late stages of endocytopsis. Interacts (via SH3 domain) with BBC1 and LAS17. Interacts (via C- terminal acidic tail) with ARC19 and ARC40; ARC19 and ARC40 are Arp2/3 complex subunits.
INTERACTION: P47068:BBC1; NbExp=3; IntAct=EBI-11670, EBI-3437; P41832:BNI1; NbExp=3; IntAct=EBI-11670, EBI-3692; P40450:BNR1; NbExp=4; IntAct=EBI-11670, EBI-3711; Q12446:LAS17; NbExp=3; IntAct=EBI-11670, EBI-10022; P37370:VRP1; NbExp=3; IntAct=EBI-11670, EBI-20502;
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch. Note=Localizes to cortical patch-like protein structures that assemble actin patches. Enriched at sites of polarized growth.
DOMAIN: The myosin head-like domain displays actin-stimulated ATPase activity and constitutes the motor domain by generating a mechanochemical force.
DOMAIN: The tail domain participates in molecular interactions that specify the role of the motor domain. It is composed of several tail homology (TH) domains, namely a putative phospholipid-binding domain (TH1), an Ala- and Pro-rich domain (TH2), followed by an SH3 domain and a C-terminal acidic domain (TH3).
PTM: Phosphorylation of the TEDS site (Ser-357) is required for the polarization of the actin cytoskeleton and for ligand-induced, but not for constitutive internalization of STE2. Phosphorylation probably activates the myosin-I ATPase (By similarity). Ser-357 is phosphorylated by CLA4 and STE20 in vitro.
MISCELLANEOUS: Present with 155 molecules/cell in log phase SD medium.
SIMILARITY: Contains 2 IQ domains.
SIMILARITY: Contains 1 myosin head-like domain.
SIMILARITY: Contains 1 SH3 domain.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001609 - Myosin_head_motor_dom
IPR010926 - Myosin_tail_2
IPR001452 - SH3_domain

Pfam Domains:
PF00063 - Myosin head (motor domain)
PF06017 - Unconventional myosin tail, actin- and lipid-binding
PF00018 - SH3 domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1RUW - X-ray 1VA7 - X-ray 2BTT - NMR

ModBase Predicted Comparative 3D Structure on P36006
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanMouseRatZebrafishD. melanogasterC. elegans
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
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    Protein SequenceProtein Sequence

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000146 microfilament motor activity
GO:0000166 nucleotide binding
GO:0003774 motor activity
GO:0003777 microtubule motor activity
GO:0003779 actin binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0016787 hydrolase activity
GO:0017022 myosin binding

Biological Process:
GO:0006887 exocytosis
GO:0006897 endocytosis
GO:0006970 response to osmotic stress
GO:0007018 microtubule-based movement
GO:0007121 bipolar cellular bud site selection
GO:0031505 fungal-type cell wall organization
GO:0051666 actin cortical patch localization
GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0016459 myosin complex
GO:0030479 actin cortical patch

-  Descriptions from all associated GenBank mRNAs
  MN048060 - Synthetic construct clone CCSBy3025B01 Gateway entry clone sequence.

-  Other Names for This Gene
Protein: P36006 (aka MYO3_YEAST or MYS3_YEAST)

-  SGD Genes Methods, Credits, and Data Use Restrictions
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