Human Gene GMEB1 (ENST00000361872.8) from GENCODE V44
  Description: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses. (from UniProt Q9Y692)
RefSeq Summary (NM_024482): This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Three alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2016].
Gencode Transcript: ENST00000361872.8
Gencode Gene: ENSG00000162419.13
Transcript (Including UTRs)
   Position: hg38 chr1:28,668,778-28,714,886 Size: 46,109 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg38 chr1:28,683,613-28,714,773 Size: 31,161 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:28,668,778-28,714,886)mRNA (may differ from genome)Protein (563 aa)
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Wikipedia

-  Comments and Description Text from UniProtKB
  ID: GMEB1_HUMAN
DESCRIPTION: RecName: Full=Glucocorticoid modulatory element-binding protein 1; Short=GMEB-1; AltName: Full=DNA-binding protein p96PIF; AltName: Full=Parvovirus initiation factor p96; Short=PIF p96;
FUNCTION: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses.
SUBUNIT: Homodimer, and heterodimer of GMEB1 and GMEB2. GMEB1 and GMEB2 form the parvovirus initiator complex (PIF). Interacts with the glucocorticoid receptor (NR3C1) and NCOA2/TIF2 (By similarity). May interact with HSP27 and CREB-binding protein (CBP).
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=May be also cytoplasmic.
SIMILARITY: Contains 1 SAND domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.54 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 99.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -26.5089-0.298 Picture PostScript Text
3' UTR -13.50113-0.119 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024830 - GMEB1/2
IPR000770 - SAND_dom
IPR010919 - SAND_dom-like

Pfam Domains:
PF01342 - SAND domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1OQJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9Y692
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
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Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003713 transcription coactivator activity
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006366 transcription from RNA polymerase II promoter
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK000892 - Homo sapiens cDNA FLJ10030 fis, clone HEMBA1000851.
LP895146 - Sequence 10 from Patent EP3253886.
BC001473 - Homo sapiens glucocorticoid modulatory element binding protein 1, mRNA (cDNA clone MGC:1001 IMAGE:3138481), complete cds.
AK315195 - Homo sapiens cDNA, FLJ96179, highly similar to Homo sapiens glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 1, mRNA.
AK315436 - Homo sapiens cDNA, FLJ96492, Homo sapiens glucocorticoid modulatory element binding protein 1(GMEB1), transcript variant 2, mRNA.
AF173868 - Homo sapiens DNA binding protein p96PIF mRNA, complete cds.
AF203694 - Homo sapiens glucocorticoid modulatory element binding protein 1 (GMEB1) mRNA, complete cds.
AK023126 - Homo sapiens cDNA FLJ13064 fis, clone NT2RP3001688, highly similar to Homo sapiens DNA binding protein p96PIF mRNA.
AF099013 - Homo sapiens glucocorticoid modulatory element binding protein-1 (GMEB1) mRNA, complete cds.
AB463689 - Synthetic construct DNA, clone: pF1KB8174, Homo sapiens GMEB1 gene for glucocorticoid modulatory element binding protein 1, without stop codon, in Flexi system.
EU446954 - Synthetic construct Homo sapiens clone IMAGE:100070166; IMAGE:100012163; FLH257438.01L glucocorticoid modulatory element binding protein 1 (GMEB1) gene, encodes complete protein.
KJ893115 - Synthetic construct Homo sapiens clone ccsbBroadEn_02509 GMEB1 gene, encodes complete protein.
JD227695 - Sequence 208719 from Patent EP1572962.
JD038240 - Sequence 19264 from Patent EP1572962.
CU674728 - Synthetic construct Homo sapiens gateway clone IMAGE:100017703 5' read GMEB1 mRNA.

-  Other Names for This Gene
  Alternate Gene Symbols: B1AT48, ENST00000361872.1, ENST00000361872.2, ENST00000361872.3, ENST00000361872.4, ENST00000361872.5, ENST00000361872.6, ENST00000361872.7, GMEB1_HUMAN, LP895146, Q9NWH1, Q9UKD0, Q9Y692, uc001bqz.1, uc001bqz.2, uc001bqz.3, uc001bqz.4
UCSC ID: ENST00000361872.8
RefSeq Accession: NM_024482
Protein: Q9Y692 (aka GMEB1_HUMAN or GME1_HUMAN)
CCDS: CCDS327.1, CCDS328.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.