Human Gene NGF (ENST00000369512.3) from GENCODE V44
  Description: Homo sapiens nerve growth factor (NGF), mRNA. (from RefSeq NM_002506)
RefSeq Summary (NM_002506): This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000369512.3
Gencode Gene: ENSG00000134259.6
Transcript (Including UTRs)
   Position: hg38 chr1:115,285,917-115,338,249 Size: 52,333 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg38 chr1:115,286,070-115,286,795 Size: 726 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesGeneReviewsMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:115,285,917-115,338,249)mRNA (may differ from genome)Protein (241 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NGF_HUMAN
DESCRIPTION: RecName: Full=Beta-nerve growth factor; Short=Beta-NGF; Flags: Precursor;
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
DISEASE: Defects in NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.
SIMILARITY: Belongs to the NGF-beta family.
SEQUENCE CAUTION: Sequence=AAH32517.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
WEB RESOURCE: Name=Wikipedia; Note=Nerve growth factor entry; URL="http://en.wikipedia.org/wiki/Nerve_growth_factor";

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NGF
Diseases sorted by gene-association score: neuropathy, hereditary sensory and autonomic, type v* (1669), autonomic neuropathy (49), corneal ulcer (27), anhidrosis (20), cystitis (19), diabetic neuropathy (19), interstitial cystitis (18), chronic cystitis (18), neuropathy (16), neuroschistosomiasis (16), cannabis abuse (14), lesion of sciatic nerve (12), allergic rhinitis (12), burning mouth syndrome (11), phaeochromocytoma (11), cough variant asthma (11), diabetic polyneuropathy (11), ataxia, cerebellar, cayman type (10), rhinitis (10), insensitivity to pain, congenital (9), ependymoblastoma (9), lesch-nyhan syndrome (9), neuroma (9), sweat gland disease (8), pheochromocytoma (8), askin's tumor (8), prurigo nodularis (8), dysautonomia, familial (8), algoneurodystrophy (8), ischemic neuropathy (8), keratoconjunctivitis (8), dry eye syndrome (7), pain disorder (7), diabetic autonomic neuropathy (7), cortical thymoma (7), bladder disease (7), ocular cicatricial pemphigoid (7), demyelinating polyneuropathy (7), keratoconjunctivitis sicca (6), opiate dependence (6), conjunctival nevus (6), prolactin producing pituitary tumor (6), autonomic nervous system neoplasm (6), sensory peripheral neuropathy (6), myelomeningocele (6), adenomyosis (6), peripheral nervous system neoplasm (6), meningeal melanomatosis (6), cerebral neuroblastoma (6), meningoencephalitis (5), central nervous system disease (5), sciatic neuropathy (5), corneal disease (5), autonomic nervous system disease (5), jejunoileitis (4), somatoform disorder (4), peripheral nervous system disease (4), toxic encephalopathy (4), cerebral cavernous malformations-2 (4), plexopathy (4), familial renal papillary carcinoma (4), ventricular fibrillation, familial, 1 (4), shprintzen-goldberg syndrome (3), ventricular tachycardia, catecholaminergic polymorphic, 1 (3), alzheimer disease (3), medulloblastoma (3), asthma (2), parkinson disease, late-onset (2), neuroblastoma (1), nervous system disease (1), amyotrophic lateral sclerosis 1 (1), multiple sclerosis, disease progression, modifier of (1), nervous system cancer (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.75 RPKM in Fallopian Tube
Total median expression: 138.34 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -74.30182-0.408 Picture PostScript Text
3' UTR -19.10153-0.125 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020408 - Nerve_growth_factor-like
IPR002072 - Nerve_growth_factor-rel
IPR020425 - Nerve_growth_factor_bsu
IPR020437 - Nerve_growth_factor_bsu_mml
IPR019846 - Nerve_growth_factor_CS

Pfam Domains:
PF00243 - Nerve growth factor family

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1SG1 - X-ray MuPIT 1WWW - X-ray MuPIT 2IFG - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P01138
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004857 enzyme inhibitor activity
GO:0005102 receptor binding
GO:0005163 nerve growth factor receptor binding
GO:0005515 protein binding
GO:0008083 growth factor activity
GO:0008191 metalloendopeptidase inhibitor activity
GO:0008656 cysteine-type endopeptidase activator activity involved in apoptotic process
GO:0030414 peptidase inhibitor activity
GO:0038177 death receptor agonist activity

Biological Process:
GO:0000186 activation of MAPKK activity
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation
GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors
GO:0010466 negative regulation of peptidase activity
GO:0010469 regulation of receptor activity
GO:0010628 positive regulation of gene expression
GO:0032455 nerve growth factor processing
GO:0043065 positive regulation of apoptotic process
GO:0043066 negative regulation of apoptotic process
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043281 regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043388 positive regulation of DNA binding
GO:0043524 negative regulation of neuron apoptotic process
GO:0045664 regulation of neuron differentiation
GO:0045666 positive regulation of neuron differentiation
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048011 neurotrophin TRK receptor signaling pathway
GO:0048015 phosphatidylinositol-mediated signaling
GO:0048812 neuron projection morphogenesis
GO:0050772 positive regulation of axonogenesis
GO:0051402 neuron apoptotic process

Cellular Component:
GO:0005576 extracellular region
GO:0005768 endosome
GO:0005796 Golgi lumen
GO:0031410 cytoplasmic vesicle


-  Descriptions from all associated GenBank mRNAs
  HH934032 - Sequence 107 from Patent WO2010111468.
HV964550 - JP 2012521762-A/107: RNA Interference Mediated Inhibition of the Nerve Growth Factor Beta Chain (NGFB) Gene Expression Using Short Interfering Nucleic Acid (siNA).
BC032517 - Homo sapiens nerve growth factor (beta polypeptide), mRNA (cDNA clone IMAGE:5493721), partial cds.
X52599 - Human mRNA for beta nerve growth factor.
JD284293 - Sequence 265317 from Patent EP1572962.
BC126148 - Homo sapiens nerve growth factor (beta polypeptide), mRNA (cDNA clone MGC:161426 IMAGE:8991864), complete cds.
BC126150 - Homo sapiens nerve growth factor (beta polypeptide), mRNA (cDNA clone MGC:161428 IMAGE:8991866), complete cds.
JD399570 - Sequence 380594 from Patent EP1572962.
E03037 - DNA encoding human nerve growth factor.
AF411526 - Homo sapiens nerve growth factor beta (NGFB) mRNA, complete cds.
JD051366 - Sequence 32390 from Patent EP1572962.
MH358394 - Homo sapiens pro-nerve growth factor long variant (NGF) mRNA, complete cds, alternatively spliced.
CR541855 - Homo sapiens full open reading frame cDNA clone RZPDo834H0532D for gene NGFB, nerve growth factor, beta polypeptide; complete cds, incl. stopcodon.
BT019733 - Homo sapiens nerve growth factor, beta polypeptide mRNA, complete cds.
HQ258125 - Synthetic construct Homo sapiens clone IMAGE:100072434 nerve growth factor (beta polypeptide) (NGF) gene, encodes complete protein.
KJ897246 - Synthetic construct Homo sapiens clone ccsbBroadEn_06640 NGF gene, encodes complete protein.
KR711649 - Synthetic construct Homo sapiens clone CCSBHm_00028069 NGF (NGF) mRNA, encodes complete protein.
KR711650 - Synthetic construct Homo sapiens clone CCSBHm_00028070 NGF (NGF) mRNA, encodes complete protein.
AB489186 - Synthetic construct DNA, clone: pF1KB9808, Homo sapiens NGF gene for nerve growth factor, without stop codon, in Flexi system.
JD343130 - Sequence 324154 from Patent EP1572962.
JD378418 - Sequence 359442 from Patent EP1572962.
JD064194 - Sequence 45218 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04010 - MAPK signaling pathway
hsa04210 - Apoptosis
hsa04722 - Neurotrophin signaling pathway

BioCarta from NCI Cancer Genome Anatomy Project
h_erkPathway - Erk1/Erk2 Mapk Signaling pathway
h_btg2Pathway - BTG family proteins and cell cycle regulation
h_trkaPathway - Trka Receptor Signaling Pathway
h_cdk5Pathway - Phosphorylation of MEK1 by cdk5/p35 down regulates the MAP kinase pathway
h_ngfPathway - Nerve growth factor pathway (NGF)

Reactome (by CSHL, EBI, and GO)

Protein P01138 (Reactome details) participates in the following event(s):

R-HSA-187045 The signal peptide is excised from beta-NGF pre-pro-precursor
R-HSA-167047 pro-beta-NGF dimerizes
R-HSA-187035 Pro-beta-NGF and mature beta-NGF are secreted
R-HSA-187020 Part of pro-beta-NGF is processed to mature beta-NGF
R-HSA-166542 beta-NGF dimer binds to TrkA receptor
R-HSA-193646 NGF binding to p75NTR inactivates RHOA
R-HSA-193653 NGF homodimer binds to p75NTR
R-HSA-193642 Binding of pro-NGF to p75NTR:sortilin
R-HSA-167014 pro-beta-NGF homodimer transits to the golgi apparatus
R-HSA-167056 SHC binds to the activated TrkA receptor
R-HSA-167674 Binding of PLCG1 to active TrkA receptor
R-HSA-169891 ARMS:Crk complex binds to active TrkA receptor
R-HSA-177491 Formation of clathrin-coated vesicle
R-HSA-187697 TRKA recruits RIT and RIN
R-HSA-198211 Active TRKA binds IRS1/2
R-HSA-166544 TrkA receptor autophosphorylates
R-HSA-167217 Phospho-Shc dissociates from the TrkA receptor
R-HSA-167684 Active PLCG1 dissociates from TrkA receptor
R-HSA-198315 Active IRS recruits PI3K to the plasma membrane and activates it
R-HSA-198295 TRKA phosphorylates IRS
R-HSA-166538 The bound receptor dimerizes
R-HSA-193643 PRDM4 (SC1) binds to p75NTR
R-HSA-193650 p75NTR binds to NADE
R-HSA-193656 p75NTR interacts with RIP2
R-HSA-193677 NRIF binds to p75NTR
R-HSA-193686 p75NTR interacts with IRAK:MYD88
R-HSA-205115 NGF:p75NTR complex binds to NRAGE
R-HSA-205112 gamma-secretase cleaves p75NTR, releasing NRIF and TRAF6
R-HSA-170964 FRS2 binds to active TrkA receptor
R-HSA-167019 SHC, complexed with TrkA, is tyrosine-phosphorylated
R-HSA-167683 TrkA phosphorylates PLCG1
R-HSA-169895 Crk's SH3 domain engages C3G
R-HSA-169905 ARMS is phosphorylated by active TrkA receptor
R-HSA-177479 Axonal transport of NGF:Trk complexes
R-HSA-177501 Endocytosis (internalization) of clathrin-coated vesicle
R-HSA-187746 RIT/RIN are activated
R-HSA-187698 RIT/RIN-GTP binds B-RAF
R-HSA-198732 STAT3 activation
R-HSA-204981 14-3-3epsilon attentuates NADE-related apoptosis
R-HSA-204958 NRAGE sequesters CHE1 in the cytoplasm
R-HSA-193669 TRAF6 binds to p75NTR:NRIF
R-HSA-193703 IKKbeta is activated
R-HSA-193694 p62 is recruited and forms a complex with TRAF6
R-HSA-193665 MYD88 dissociates
R-HSA-193647 IRAK is activated
R-HSA-193695 IRAK interacts with TRAF6
R-HSA-193672 Sphingomyelinase is activated by the NGF:p75NTR complex
R-HSA-170977 FRS2 is phosphorylated by active TrkA receptor
R-HSA-170975 p-FRS2 binds CRKL
R-HSA-170978 Phospho-Frs2:CrkL binds C3G
R-HSA-169901 (ARMS)Rap1-GTP binds and activates B-Raf
R-HSA-169904 C3G stimulates nucleotide exchange on Rap1
R-HSA-205117 p75NTR:NADE promotes caspase2/3 activation
R-HSA-205132 NRAGE activates JNK
R-HSA-193705 IKKbeta phosphorylates IkB causing NF-kB to dissociate
R-HSA-193641 IKK-beta is recruited
R-HSA-193684 p62 recruits an atypical PKC
R-HSA-204949 NRIF and TRAF6 may activate JNK
R-HSA-170979 (Frs2)C3G stimulates nucleotide exchange on Rap1
R-HSA-167060 NGF processing
R-HSA-187042 TRKA activation by NGF
R-HSA-209563 Axonal growth stimulation
R-HSA-205017 NFG and proNGF binds to p75NTR
R-HSA-9036866 Expression and Processing of Neurotrophins
R-HSA-167044 Signalling to RAS
R-HSA-167021 PLC-gamma1 signalling
R-HSA-170984 ARMS-mediated activation
R-HSA-177504 Retrograde neurotrophin signalling
R-HSA-187706 Signalling to p38 via RIT and RIN
R-HSA-198203 PI3K/AKT activation
R-HSA-193670 p75NTR negatively regulates cell cycle via SC1
R-HSA-205025 NADE modulates death signalling
R-HSA-209543 p75NTR recruits signalling complexes
R-HSA-205043 NRIF signals cell death from the nucleus
R-HSA-193648 NRAGE signals death through JNK
R-HSA-187015 Activation of TRKA receptors
R-HSA-193697 p75NTR regulates axonogenesis
R-HSA-193704 p75 NTR receptor-mediated signalling
R-HSA-166520 Signaling by NTRKs
R-HSA-170968 Frs2-mediated activation
R-HSA-187687 Signalling to ERKs
R-HSA-187037 Signaling by NTRK1 (TRKA)
R-HSA-169893 Prolonged ERK activation events
R-HSA-198745 Signalling to STAT3
R-HSA-209560 NF-kB is activated and signals survival
R-HSA-204998 Cell death signalling via NRAGE, NRIF and NADE
R-HSA-193639 p75NTR signals via NF-kB
R-HSA-193681 Ceramide signalling
R-HSA-73887 Death Receptor Signalling
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A1A4E5, ENST00000369512.1, ENST00000369512.2, NGFB, NGF_HUMAN, NM_002506, P01138, Q6FHA0, Q96P60, Q9P2Q8, Q9UKL8, uc001efu.1, uc001efu.2, uc001efu.3
UCSC ID: ENST00000369512.3
RefSeq Accession: NM_002506
Protein: P01138 (aka NGF_HUMAN)
CCDS: CCDS882.1

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene NGF:
cip-overview (Congenital Insensitivity to Pain Overview)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.