Human Gene CD34 (ENST00000356522.4) from GENCODE V44
  Description: Homo sapiens CD34 molecule (CD34), transcript variant 2, mRNA. (from RefSeq NM_001773)
RefSeq Summary (NM_001773): The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Gencode Transcript: ENST00000356522.4
Gencode Gene: ENSG00000174059.17
Transcript (Including UTRs)
   Position: hg38 chr1:207,886,539-207,911,402 Size: 24,864 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg38 chr1:207,888,104-207,911,080 Size: 22,977 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:207,886,539-207,911,402)mRNA (may differ from genome)Protein (328 aa)
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CD34_HUMAN
DESCRIPTION: RecName: Full=Hematopoietic progenitor cell antigen CD34; AltName: CD_antigen=CD34; Flags: Precursor;
FUNCTION: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated.
PTM: Phosphorylated on serine residues by PKC.
SIMILARITY: Belongs to the CD34 family.
SEQUENCE CAUTION: Sequence=AAA03181.1; Type=Erroneous initiation;
WEB RESOURCE: Name=Wikipedia; Note=CD34 entry; URL="http://en.wikipedia.org/wiki/CD34";

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CD34
Diseases sorted by gene-association score: dermatofibrosarcoma protuberans (10), neurofibroma (6), gastrointestinal stromal tumor (6), spindle cell lipoma (6), fibroma (5), acute leukemia (5), middle ear squamous cell carcinoma (5), hypercalcemic type ovarian small cell carcinoma (5), benign fibrous mesothelioma (4), pacinian tumor (4), gastric liposarcoma (4), vulvar proximal-type epithelioid sarcoma (4), central epithelioid sarcoma (4), leiomyoma (4), myelodysplastic syndrome (4), atypical neurofibroma (4), hemangioma (4), liposarcoma (4), aplastic anemia (4), malignant peripheral nerve sheath tumor (3), leukemia, chronic myeloid, somatic (3), testicular fibroma (3), breast myofibroblastoma (3), ovarian angiosarcoma (3), ovary sarcoma (3), hepatocellular carcinoma (3), epithelioid neurofibroma (3), lung leiomyosarcoma (3), pineal region choriocarcinoma (3), prostate stromal sarcoma (3), mechanical ectropion (3), acquired immunodeficiency syndrome (3), gallbladder leiomyoma (3), kaposiform hemangioendothelioma (3), idiopathic interstitial pneumonia (3), cellular neurofibroma (3), kidney hemangiopericytoma (2), hobnail hemangioma (2), chronic neutrophilic leukemia (2), leukemia, acute lymphoblastic (2), glomangiomyoma (2), connective tissue cancer (2), t-cell adult acute lymphocytic leukemia (2), intravascular fasciitis (2), multiple myeloma (2), mesenchymal cell neoplasm (2), malignant pineal area germ cell neoplasm (2), integumentary system benign neoplasm (2), pediatric angiosarcoma (2), pleomorphic lipoma (2), adult mesoblastic nephroma (2), retina lymphoma (2), vulvar dystrophy (2), ovarian solid teratoma (2), tibial adamantinoma (2), liver leiomyoma (2), synovium cancer (2), nervous system benign neoplasm (2), small intestinal sarcoma (2), thyroid angiosarcoma (2), organ system benign neoplasm (2), small intestine leiomyosarcoma (2), cell type benign neoplasm (2), trigonitis (2), combined t cell and b cell immunodeficiency (2), exophthalmos (2), plexiform schwannoma (2), leukocyte disease (2), adult xanthogranuloma (2), deep leiomyoma (2), conventional angiosarcoma (2), melanotic neurilemmoma (2), atypical lipomatous tumor (2), breast angiosarcoma (2), angiomyoma (2), muscle cancer (2), breast benign neoplasm (2), thoracic benign neoplasm (2), spindle cell liposarcoma (1), neurogenic arthropathy (1), meningioma, familial (1), peripheral nervous system neoplasm (1), gallbladder sarcoma (1), lung meningioma (1), glomeruloid hemangioma (1), spleen angiosarcoma (1), breast leiomyosarcoma (1), lymphoma, non-hodgkin (1), bone epithelioid hemangioma (1), immune system disease (1), extracutaneous mastocytoma (1), vulvar sarcoma (1), spleen cancer (1), leukemia, acute myeloid (1), cell type cancer (1), liver inflammatory pseudotumor (1), psammomatous meningioma (1), erythema elevatum diutinum (1), conventional fibrosarcoma (1), sternum cancer (1), orbital granuloma (1), myelofibrosis with myeloid metaplasia, somatic (1), lymphangiosarcoma (1), thyroid sarcoma (1), meninges sarcoma (1), venous hemangioma (1), myocardial infarction (1), meninges hemangiopericytoma (1), paraphimosis (1), cardiovascular organ benign neoplasm (1), benign perivascular tumor (1), ischemic fasciitis (1), gastrointestinal system cancer (1), cellular schwannoma (1), interstitial emphysema (1), invasive malignant thymoma (1), extraosseous osteosarcoma (1), infiltrating lipoma (1), hemangioma of liver (1), hemangioma of intra-abdominal structure (1), diffuse cutaneous mastocytosis (1), prostate leiomyosarcoma (1), nervous system cancer (1), mixed liposarcoma (1), internal hemorrhoid (1), sarcomatoid squamous cell skin carcinoma (1), sclerosing liposarcoma (1), diffuse meningeal melanocytosis (1), eccrine adenocarcinoma (1), chorioangioma (1), glomangioma (1), deep angioma (1), chronic monocytic leukemia (1), liver angiosarcoma (1), nephrogenic adenofibroma (1), aleukemic leukemia cutis (1), intraneural perineurioma (1), proliferative fasciitis (1), skin sarcoma (1), clear cell ependymoma (1), congenital epulis (1), intramuscular hemangioma (1), pediatric lymphoma (1), spindle cell synovial sarcoma (1), reticulohistiocytic granuloma (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 97.13 RPKM in Adipose - Subcutaneous
Total median expression: 1200.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -129.50322-0.402 Picture PostScript Text
3' UTR -494.301565-0.316 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008083 - CD34
IPR013836 - CD34/Podocalyxin

Pfam Domains:
PF06365 - CD34/Podocalyxin family

ModBase Predicted Comparative 3D Structure on P28906
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008134 transcription factor binding
GO:0030246 carbohydrate binding
GO:0043199 sulfate binding

Biological Process:
GO:0001894 tissue homeostasis
GO:0001935 endothelial cell proliferation
GO:0003094 glomerular filtration
GO:0003158 endothelium development
GO:0007155 cell adhesion
GO:0007160 cell-matrix adhesion
GO:0007165 signal transduction
GO:0008217 regulation of blood pressure
GO:0008283 cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010629 negative regulation of gene expression
GO:0030097 hemopoiesis
GO:0030195 negative regulation of blood coagulation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0032733 positive regulation of interleukin-10 production
GO:0035759 mesangial cell-matrix adhesion
GO:0038001 paracrine signaling
GO:0042482 positive regulation of odontogenesis
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0048870 cell motility
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0060290 transdifferentiation
GO:0061042 vascular wound healing
GO:0071425 hematopoietic stem cell proliferation
GO:0071636 positive regulation of transforming growth factor beta production
GO:0071657 positive regulation of granulocyte colony-stimulating factor production
GO:0071971 extracellular exosome assembly
GO:0072011 glomerular endothelium development
GO:0072089 stem cell proliferation
GO:0072254 metanephric glomerular mesangial cell differentiation
GO:0098609 cell-cell adhesion
GO:1900035 negative regulation of cellular response to heat
GO:1900038 negative regulation of cellular response to hypoxia
GO:1900041 negative regulation of interleukin-2 secretion
GO:1900168 positive regulation of glial cell-derived neurotrophic factor secretion
GO:1901215 negative regulation of neuron death
GO:2001214 positive regulation of vasculogenesis

Cellular Component:
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0009897 external side of plasma membrane
GO:0009925 basal plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0016324 apical plasma membrane
GO:0036053 glomerular endothelium fenestra
GO:0045171 intercellular bridge
GO:0048471 perinuclear region of cytoplasm
GO:0071944 cell periphery


-  Descriptions from all associated GenBank mRNAs
  BX538076 - Homo sapiens mRNA; cDNA DKFZp686I22213 (from clone DKFZp686I22213).
BC039146 - Homo sapiens CD34 molecule, mRNA (cDNA clone MGC:21247 IMAGE:4746591), complete cds.
S53910 - CD34=glycoprotein expressed in lymphohematopoietic progenitor cells {alternatively spliced} [human, UT7 cells, mRNA, 2463 nt].
S53911 - CD34=glycoprotein expressed in lymphohematopoietic progenitor cells {alternatively spliced, truncated form} [human, UT7, mRNA, 2657 nt].
AK091890 - Homo sapiens cDNA FLJ34571 fis, clone KIDNE2008116, highly similar to HEMATOPOIETIC PROGENITOR CELL ANTIGEN CD34 PRECURSOR.
LQ882868 - Sequence 17 from Patent WO2018160841.
M81104 - Homo sapiens CD34 mRNA, complete cds.
DM110847 - Novel Cancer Marker and Use Thereof.
JD166224 - Sequence 147248 from Patent EP1572962.
JD393887 - Sequence 374911 from Patent EP1572962.
JD382744 - Sequence 363768 from Patent EP1572962.
JD126706 - Sequence 107730 from Patent EP1572962.
JD291205 - Sequence 272229 from Patent EP1572962.
JD380020 - Sequence 361044 from Patent EP1572962.
JD271220 - Sequence 252244 from Patent EP1572962.
JD399665 - Sequence 380689 from Patent EP1572962.
JD519444 - Sequence 500468 from Patent EP1572962.
JD398450 - Sequence 379474 from Patent EP1572962.
JD119661 - Sequence 100685 from Patent EP1572962.
JD274064 - Sequence 255088 from Patent EP1572962.
JD445218 - Sequence 426242 from Patent EP1572962.
JD524484 - Sequence 505508 from Patent EP1572962.
JD374113 - Sequence 355137 from Patent EP1572962.
JD133172 - Sequence 114196 from Patent EP1572962.
JD545681 - Sequence 526705 from Patent EP1572962.
JD168988 - Sequence 150012 from Patent EP1572962.
JD234319 - Sequence 215343 from Patent EP1572962.
JD202705 - Sequence 183729 from Patent EP1572962.
JD528843 - Sequence 509867 from Patent EP1572962.
AK294381 - Homo sapiens cDNA FLJ53734 complete cds, highly similar to Hematopoietic progenitor cell antigen CD34 precursor.
JD241940 - Sequence 222964 from Patent EP1572962.
JD298204 - Sequence 279228 from Patent EP1572962.
AK291529 - Homo sapiens cDNA FLJ78715 complete cds, highly similar to Homo sapiens CD34 antigen (CD34), transcript variant 1, mRNA.
JD390293 - Sequence 371317 from Patent EP1572962.
JD253490 - Sequence 234514 from Patent EP1572962.
AK293030 - Homo sapiens cDNA FLJ75215 complete cds, highly similar to CD34=glycoprotein expressed in lymphohematopoietic progenitor cells.
JD389631 - Sequence 370655 from Patent EP1572962.
JD372720 - Sequence 353744 from Patent EP1572962.
JD449810 - Sequence 430834 from Patent EP1572962.
JD434864 - Sequence 415888 from Patent EP1572962.
JD261648 - Sequence 242672 from Patent EP1572962.
AF523361 - Homo sapiens CD34 antigen (CD34) mRNA, complete cds.
JD402266 - Sequence 383290 from Patent EP1572962.
JD120744 - Sequence 101768 from Patent EP1572962.
JD264225 - Sequence 245249 from Patent EP1572962.
AK300835 - Homo sapiens cDNA FLJ54166 complete cds, highly similar to Hematopoietic progenitor cell antigen CD34 precursor.
DQ891428 - Synthetic construct clone IMAGE:100004058; FLH176854.01X; RZPDo839A03124D CD34 molecule (CD34) gene, encodes complete protein.
DQ894603 - Synthetic construct Homo sapiens clone IMAGE:100009063; FLH176851.01L; RZPDo839A03123D CD34 molecule (CD34) gene, encodes complete protein.
LQ493928 - Sequence 7 from Patent WO2016077734.
JD394893 - Sequence 375917 from Patent EP1572962.
JD213511 - Sequence 194535 from Patent EP1572962.
AF202879 - Homo sapiens hematopoietic progenitor cell antigen CD34 precursor (CD34) mRNA, partial cds.
JD390820 - Sequence 371844 from Patent EP1572962.
JD285749 - Sequence 266773 from Patent EP1572962.
JD278097 - Sequence 259121 from Patent EP1572962.
JD392750 - Sequence 373774 from Patent EP1572962.
JD397637 - Sequence 378661 from Patent EP1572962.
JD389451 - Sequence 370475 from Patent EP1572962.
JD252117 - Sequence 233141 from Patent EP1572962.
JD338659 - Sequence 319683 from Patent EP1572962.
JD416612 - Sequence 397636 from Patent EP1572962.
JD080379 - Sequence 61403 from Patent EP1572962.
JD328518 - Sequence 309542 from Patent EP1572962.
AF538601 - Homo sapiens hematopoietic progenital cell antigen CD34 precursor (CD34) mRNA, partial cds.
JD196354 - Sequence 177378 from Patent EP1572962.
JD336618 - Sequence 317642 from Patent EP1572962.
MP584518 - Sequence 17 from Patent WO2020081767.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04514 - Cell adhesion molecules (CAMs)
hsa04640 - Hematopoietic cell lineage

BioCarta from NCI Cancer Genome Anatomy Project
h_lympathway - Adhesion and Diapedesis of Lymphocytes
h_il17Pathway - IL 17 Signaling Pathway

Reactome (by CSHL, EBI, and GO)

Protein P28906 (Reactome details) participates in the following event(s):

R-HSA-199046 Ligands bind L-selectin
R-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A8K664, CD34_HUMAN, ENST00000356522.1, ENST00000356522.2, ENST00000356522.3, NM_001773, P28906, Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1, uc001hgx.1, uc001hgx.2
UCSC ID: ENST00000356522.4
RefSeq Accession: NM_001773
Protein: P28906 (aka CD34_HUMAN)
CCDS: CCDS31012.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.