Human Gene MAP3K21 (ENST00000366622.1) from GENCODE V44
  Description: Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways. (from UniProt Q5TCX8)
Gencode Transcript: ENST00000366622.1
Gencode Gene: ENSG00000143674.11
Transcript (Including UTRs)
   Position: hg38 chr1:233,375,783-233,385,147 Size: 9,365 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr1:233,375,903-233,382,711 Size: 6,809 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:233,375,783-233,385,147)mRNA (may differ from genome)Protein (482 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHPRD
LynxMGIneXtProtPubMedUniProtKB

-  Comments and Description Text from UniProtKB
  ID: M3KL4_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase kinase kinase MLK4; EC=2.7.11.25; AltName: Full=Mixed lineage kinase 4;
FUNCTION: Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation (By similarity).
SUBUNIT: Homodimer (By similarity). Interacts with TLR4.
PTM: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 SH3 domain.
SEQUENCE CAUTION: Sequence=BAB47433.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.37 RPKM in Stomach
Total median expression: 54.05 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.20120-0.135 Picture PostScript Text
3' UTR -530.002436-0.218 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR016231 - MAPKKK9/10/11
IPR015785 - MAPKKK9/10/11-like
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS
IPR001452 - SH3_domain
IPR020635 - Tyr_kinase_cat_dom

Pfam Domains:
PF07714 - Protein tyrosine kinase
PF00018 - SH3 domain

ModBase Predicted Comparative 3D Structure on Q5TCX8
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004706 JUN kinase kinase kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity

Biological Process:
GO:0000186 activation of MAPKK activity
GO:0006468 protein phosphorylation
GO:0007256 activation of JNKK activity
GO:0007257 activation of JUN kinase activity
GO:0016310 phosphorylation
GO:0046777 protein autophosphorylation

Cellular Component:
GO:0005575 cellular_component
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AJ311798 - Homo sapiens mRNA for mixed lineage kinase 4beta (MLK4BETA gene).
BC136648 - Homo sapiens mixed lineage kinase 4, mRNA (cDNA clone MGC:168261 IMAGE:9020638), complete cds.
BC136649 - Homo sapiens mixed lineage kinase 4, mRNA (cDNA clone MGC:168262 IMAGE:9020639), complete cds.
AB058707 - Homo sapiens mRNA for KIAA1804 protein, partial cds.
AK309811 - Homo sapiens cDNA, FLJ99852.
JD435734 - Sequence 416758 from Patent EP1572962.
JD176304 - Sequence 157328 from Patent EP1572962.
JD037256 - Sequence 18280 from Patent EP1572962.
JD458165 - Sequence 439189 from Patent EP1572962.
JD251298 - Sequence 232322 from Patent EP1572962.
JD303164 - Sequence 284188 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AB058707, B2RN34, KIAA1804, M3KL4_HUMAN, MLK4, Q5TCX7, Q5TCX8, Q5TCX9, Q8WWN1, Q8WWN2, Q96JM1, uc001hvu.1, uc001hvu.2, uc001hvu.3, uc001hvu.4, uc001hvu.5
UCSC ID: ENST00000366622.1
RefSeq Accession: NM_032435
Protein: Q5TCX8 (aka M3KL4_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.