Human Gene MRPL43 (ENST00000370234.4) from GENCODE V44
Description: Homo sapiens mitochondrial ribosomal protein L43 (MRPL43), transcript variant 5, mRNA; nuclear gene for mitochondrial product. (from RefSeq NM_001308396) RefSeq Summary (NM_001308396): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000370234.4 Gencode Gene: ENSG00000055950.17 Transcript (Including UTRs) Position: hg38 chr10:100,986,280-100,987,483 Size: 1,204 Total Exon Count: 4 Strand: - Coding Region Position: hg38 chr10:100,986,543-100,987,443 Size: 901 Coding Exon Count: 4
ID:RM43_HUMAN DESCRIPTION: RecName: Full=39S ribosomal protein L43, mitochondrial; Short=L43mt; Short=MRP-L43; AltName: Full=Mitochondrial ribosomal protein bMRP36a; Flags: Precursor; SUBCELLULAR LOCATION: Mitochondrion. TISSUE SPECIFICITY: High relative levels in skeletal muscle and testis. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with PEO1. SIMILARITY: Belongs to the ribosomal protein S25/L51 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8N983
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF211344 - JP 2014500723-A/18847: Polycomb-Associated Non-Coding RNAs. MA446921 - JP 2018138019-A/18847: Polycomb-Associated Non-Coding RNAs. AK095556 - Homo sapiens cDNA FLJ38237 fis, clone FCBBF2005637. BC041165 - Homo sapiens mitochondrial ribosomal protein L43, mRNA (cDNA clone MGC:48892 IMAGE:5590251), complete cds. AK300237 - Homo sapiens cDNA FLJ61386 complete cds, highly similar to Homo sapiens mitochondrial ribosomal protein L43 (MRPL43), transcript variant 4, mRNA. CU691974 - Synthetic construct Homo sapiens gateway clone IMAGE:100021069 5' read MRPL43 mRNA. HQ448543 - Synthetic construct Homo sapiens clone IMAGE:100071979; CCSB011293_01 mitochondrial ribosomal protein L43 (MRPL43) gene, encodes complete protein. KJ895004 - Synthetic construct Homo sapiens clone ccsbBroadEn_04398 MRPL43 gene, encodes complete protein. AF086122 - Homo sapiens full length insert cDNA clone ZA78A09. BC053373 - Homo sapiens mitochondrial ribosomal protein L43, mRNA (cDNA clone MGC:61563 IMAGE:6020112), complete cds. CU690970 - Synthetic construct Homo sapiens gateway clone IMAGE:100021577 5' read MRPL43 mRNA. KJ906369 - Synthetic construct Homo sapiens clone ccsbBroadEn_16039 MRPL43 gene, encodes complete protein. BC052639 - Homo sapiens mitochondrial ribosomal protein L43, mRNA (cDNA clone MGC:61576 IMAGE:6501303), complete cds. AB049656 - Homo sapiens bMRP36a mRNA for mitochondrial ribosomal protein bMRP36a, complete cds. BC015905 - Homo sapiens mitochondrial ribosomal protein L43, mRNA (cDNA clone MGC:17989 IMAGE:3921157), complete cds. BC031287 - Homo sapiens mitochondrial ribosomal protein L43, mRNA (cDNA clone IMAGE:5299038), partial cds. JD057578 - Sequence 38602 from Patent EP1572962. JD429820 - Sequence 410844 from Patent EP1572962. DQ595212 - Homo sapiens piRNA piR-61324, complete sequence. JD159979 - Sequence 141003 from Patent EP1572962. JD488264 - Sequence 469288 from Patent EP1572962. JD303375 - Sequence 284399 from Patent EP1572962. JD264893 - Sequence 245917 from Patent EP1572962. KJ906368 - Synthetic construct Homo sapiens clone ccsbBroadEn_16038 MRPL43 gene, encodes complete protein. JD400289 - Sequence 381313 from Patent EP1572962. JD537520 - Sequence 518544 from Patent EP1572962. AK291069 - Homo sapiens cDNA FLJ76392 complete cds, highly similar to Homo sapiens mitochondrial ribosomal protein L43 (MRPL43), transcript variant 1, mRNA. CU677499 - Synthetic construct Homo sapiens gateway clone IMAGE:100021045 5' read MRPL43 mRNA. KJ906367 - Synthetic construct Homo sapiens clone ccsbBroadEn_16037 MRPL43 gene, encodes complete protein. CU690928 - Synthetic construct Homo sapiens gateway clone IMAGE:100021574 5' read MRPL43 mRNA.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q8N983 (Reactome details) participates in the following event(s):
R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation) R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA) R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA R-HSA-5368286 Mitochondrial translation initiation R-HSA-5419276 Mitochondrial translation termination R-HSA-5368287 Mitochondrial translation R-HSA-5389840 Mitochondrial translation elongation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins