Human Gene LGALS12 (ENST00000394618.9) from GENCODE V44
  Description: Homo sapiens galectin 12 (LGALS12), transcript variant 2, mRNA. (from RefSeq NM_033101)
RefSeq Summary (NM_033101): This gene encodes a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
Gencode Transcript: ENST00000394618.9
Gencode Gene: ENSG00000133317.16
Transcript (Including UTRs)
   Position: hg38 chr11:63,506,084-63,516,772 Size: 10,689 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg38 chr11:63,506,459-63,516,393 Size: 9,935 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:63,506,084-63,516,772)mRNA (may differ from genome)Protein (314 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: LEG12_HUMAN
DESCRIPTION: RecName: Full=Galectin-12; Short=Gal-12; AltName: Full=Galectin-related inhibitor of proliferation;
FUNCTION: Binds lactose. May participate in the apoptosis of adipocytes.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Not widely expressed. Predominantly expressed in adipose tissue.
DOMAIN: Contains two homologous but distinct carbohydrate-binding domains.
SIMILARITY: Contains 2 galectin domains.
WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding; Note=Galectin-12; URL="http://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_hum_Stlect_278";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.22 RPKM in Adipose - Subcutaneous
Total median expression: 65.69 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -164.50375-0.439 Picture PostScript Text
3' UTR -116.30379-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008985 - ConA-like_lec_gl_sf
IPR013320 - ConA-like_subgrp
IPR001079 - Galectin_CRD

Pfam Domains:
PF00337 - Galactoside-binding lectin

ModBase Predicted Comparative 3D Structure on Q96DT0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0030246 carbohydrate binding
GO:0030395 lactose binding

Biological Process:
GO:0006915 apoptotic process
GO:0045598 regulation of fat cell differentiation
GO:0050994 regulation of lipid catabolic process
GO:0097193 intrinsic apoptotic signaling pathway

Cellular Component:
GO:0005634 nucleus
GO:0005739 mitochondrion


-  Descriptions from all associated GenBank mRNAs
  AK313851 - Homo sapiens cDNA, FLJ94479, Homo sapiens lectin, galactoside-binding, soluble, 12 (galectin 12)(LGALS12), mRNA.
HM005566 - Homo sapiens clone HTL-S-26 testicular secretory protein Li 26 mRNA, complete cds.
AF244974 - Homo sapiens galectin-12 isoform a mRNA, complete cds.
AF244976 - Homo sapiens galectin-12 isoform c mRNA, complete cds.
BC028222 - Homo sapiens lectin, galactoside-binding, soluble, 12, mRNA (cDNA clone MGC:39852 IMAGE:5217281), complete cds.
JD456215 - Sequence 437239 from Patent EP1572962.
JD295106 - Sequence 276130 from Patent EP1572962.
JD498977 - Sequence 480001 from Patent EP1572962.
JD456931 - Sequence 437955 from Patent EP1572962.
JF432638 - Synthetic construct Homo sapiens clone IMAGE:100073875 lectin, galactoside-binding, soluble, 12 (LGALS12) gene, encodes complete protein.
KJ895083 - Synthetic construct Homo sapiens clone ccsbBroadEn_04477 LGALS12 gene, encodes complete protein.
AF310686 - Homo sapiens galectin-12 splice form 1 mRNA, complete cds, alternatively spliced.
AF310687 - Homo sapiens galectin-12 splice form 2 mRNA, complete cds, alternatively spliced.
AF244975 - Homo sapiens galectin-12 isoform b mRNA, complete cds.
AF244977 - Homo sapiens galectin-12 isoform d mRNA, complete cds.
AF222694 - Homo sapiens galectin-related inhibitor of proliferation isoform b (GRIP1) mRNA, complete cds.
AF222695 - Homo sapiens galectin-related inhibitor of proliferation isoform a (GRIP1) mRNA, complete cds.
JD487134 - Sequence 468158 from Patent EP1572962.
JD126868 - Sequence 107892 from Patent EP1572962.
JD041034 - Sequence 22058 from Patent EP1572962.
JD138068 - Sequence 119092 from Patent EP1572962.
JD303904 - Sequence 284928 from Patent EP1572962.
JD500405 - Sequence 481429 from Patent EP1572962.
JD435574 - Sequence 416598 from Patent EP1572962.
JD308408 - Sequence 289432 from Patent EP1572962.
JD325703 - Sequence 306727 from Patent EP1572962.
JD162988 - Sequence 144012 from Patent EP1572962.
JD108713 - Sequence 89737 from Patent EP1572962.
JD194129 - Sequence 175153 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2R9N2, ENST00000394618.1, ENST00000394618.2, ENST00000394618.3, ENST00000394618.4, ENST00000394618.5, ENST00000394618.6, ENST00000394618.7, ENST00000394618.8, GRIP1, LEG12_HUMAN, NM_033101, Q96DS9, Q96DT0, Q96PR9, Q9H258, Q9H259, Q9NZ02, uc001nxa.1, uc001nxa.2, uc001nxa.3, uc001nxa.4
UCSC ID: ENST00000394618.9
RefSeq Accession: NM_033101
Protein: Q96DT0 (aka LEG12_HUMAN or LEGC_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.