Human Gene ESAM (ENST00000278927.10) from GENCODE V44
  Description: Homo sapiens endothelial cell adhesion molecule (ESAM), mRNA. (from RefSeq NM_138961)
Gencode Transcript: ENST00000278927.10
Gencode Gene: ENSG00000149564.12
Transcript (Including UTRs)
   Position: hg38 chr11:124,753,126-124,762,290 Size: 9,165 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr11:124,753,646-124,762,154 Size: 8,509 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:124,753,126-124,762,290)mRNA (may differ from genome)Protein (390 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ESAM_HUMAN
DESCRIPTION: RecName: Full=Endothelial cell-selective adhesion molecule; Flags: Precursor;
FUNCTION: Can mediate aggregation most likely through a homophilic molecular interaction (By similarity).
SUBUNIT: Interacts with BAIAP1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, adherens junction (By similarity). Cell junction, tight junction (By similarity). Cell membrane; Single-pass type I membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in endothelial cells.
SIMILARITY: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 124.36 RPKM in Thyroid
Total median expression: 1551.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -66.80136-0.491 Picture PostScript Text
3' UTR -157.10520-0.302 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR003599 - Ig_sub
IPR003598 - Ig_sub2
IPR013106 - Ig_V-set

Pfam Domains:
PF07686 - Immunoglobulin V-set domain

ModBase Predicted Comparative 3D Structure on Q96AP7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0007155 cell adhesion
GO:0007156 homophilic cell adhesion via plasma membrane adhesion molecules
GO:0050900 leukocyte migration
GO:0098609 cell-cell adhesion

Cellular Component:
GO:0005886 plasma membrane
GO:0005912 adherens junction
GO:0005923 bicellular tight junction
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030054 cell junction


-  Descriptions from all associated GenBank mRNAs
  KJ895142 - Synthetic construct Homo sapiens clone ccsbBroadEn_04536 ESAM gene, encodes complete protein.
AK301160 - Homo sapiens cDNA FLJ58430 complete cds, moderately similar to Endothelial cell-selective adhesion molecule precursor.
AK091023 - Homo sapiens cDNA FLJ33704 fis, clone BRAWH2005812.
AX746736 - Sequence 261 from Patent EP1308459.
AK092429 - Homo sapiens cDNA FLJ35110 fis, clone PLACE6007050.
AX747541 - Sequence 1066 from Patent EP1308459.
BC016868 - Homo sapiens endothelial cell adhesion molecule, mRNA (cDNA clone MGC:17599 IMAGE:3850665), complete cds.
AK075396 - Homo sapiens cDNA PSEC0086 fis, clone PLACE1000456, moderately similar to Endothelial cell-selective adhesion molecule.
AF361746 - Homo sapiens endothelial cell-selective adhesion molecule (ESAM) mRNA, complete cds.
AY358382 - Homo sapiens clone DNA35639 ISLP220 (UNQ220) mRNA, complete cds.
JD307688 - Sequence 288712 from Patent EP1572962.
AF277292 - Homo sapiens C4orf1 mRNA.
AY189281 - Homo sapiens LP4791 protein mRNA, complete cds.
JD381375 - Sequence 362399 from Patent EP1572962.
JD422177 - Sequence 403201 from Patent EP1572962.
JD043983 - Sequence 25007 from Patent EP1572962.
JD053634 - Sequence 34658 from Patent EP1572962.
JD043229 - Sequence 24253 from Patent EP1572962.
JD232438 - Sequence 213462 from Patent EP1572962.
JD137362 - Sequence 118386 from Patent EP1572962.
AK314710 - Homo sapiens cDNA, FLJ95567.
DQ891768 - Synthetic construct clone IMAGE:100004398; FLH179753.01X; RZPDo839C05132D endothelial cell adhesion molecule (ESAM) gene, encodes complete protein.
DQ894952 - Synthetic construct Homo sapiens clone IMAGE:100009412; FLH179749.01L; RZPDo839C05131D endothelial cell adhesion molecule (ESAM) gene, encodes complete protein.
CU677755 - Synthetic construct Homo sapiens gateway clone IMAGE:100017156 5' read ESAM mRNA.
AK127359 - Homo sapiens cDNA FLJ45433 fis, clone BRHIP3040878.
JD445696 - Sequence 426720 from Patent EP1572962.
JD392472 - Sequence 373496 from Patent EP1572962.
JD392471 - Sequence 373495 from Patent EP1572962.
JD390803 - Sequence 371827 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04514 - Cell adhesion molecules (CAMs)
hsa04670 - Leukocyte transendothelial migration

Reactome (by CSHL, EBI, and GO)

Protein Q96AP7 (Reactome details) participates in the following event(s):

R-HSA-202724 P-selectin binds P-selectin ligand
R-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000278927.1, ENST00000278927.2, ENST00000278927.3, ENST00000278927.4, ENST00000278927.5, ENST00000278927.6, ENST00000278927.7, ENST00000278927.8, ENST00000278927.9, ESAM_HUMAN, NM_138961, Q96AP7, Q96T50, uc001qav.1, uc001qav.2, uc001qav.3, uc001qav.4, uc001qav.5, uc001qav.6, UNQ220/PRO246
UCSC ID: ENST00000278927.10
RefSeq Accession: NM_138961
Protein: Q96AP7 (aka ESAM_HUMAN)
CCDS: CCDS8453.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.