Human Gene PUS7L (ENST00000416848.6) from GENCODE V44
  Description: tRNA uridine = tRNA pseudouridine. (from UniProt Q9H0K6)
Gencode Transcript: ENST00000416848.6
Gencode Gene: ENSG00000129317.15
Transcript (Including UTRs)
   Position: hg38 chr12:43,728,607-43,758,758 Size: 30,152 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg38 chr12:43,730,376-43,755,245 Size: 24,870 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:43,728,607-43,758,758)mRNA (may differ from genome)Protein (701 aa)
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UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: PUS7L_HUMAN
DESCRIPTION: RecName: Full=Pseudouridylate synthase 7 homolog-like protein; EC=5.4.99.-;
CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
SIMILARITY: Belongs to the pseudouridine synthase TruD family.
SIMILARITY: Contains 1 TRUD domain.
SEQUENCE CAUTION: Sequence=AAH20781.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.37 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 79.25 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -166.10489-0.340 Picture PostScript Text
3' UTR -404.201769-0.228 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020103 - PsdUridine_synth_cat_dom
IPR001656 - PsdUridine_synth_TruD
IPR017091 - PsdUridine_synth_TruD_euk
IPR011760 - PsdUridine_synth_TruD_insert

Pfam Domains:
PF01142 - tRNA pseudouridine synthase D (TruD)

ModBase Predicted Comparative 3D Structure on Q9H0K6
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
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Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0009982 pseudouridine synthase activity
GO:0016853 isomerase activity

Biological Process:
GO:0001522 pseudouridine synthesis
GO:0008033 tRNA processing
GO:0009451 RNA modification


-  Descriptions from all associated GenBank mRNAs
  BX647494 - Homo sapiens mRNA; cDNA DKFZp686P10128 (from clone DKFZp686P10128).
BC068502 - Homo sapiens pseudouridylate synthase 7 homolog (S. cerevisiae)-like, mRNA (cDNA clone MGC:87243 IMAGE:5259952), complete cds.
AL136759 - Homo sapiens mRNA; cDNA DKFZp434G1415 (from clone DKFZp434G1415); complete cds.
JD345124 - Sequence 326148 from Patent EP1572962.
JD277938 - Sequence 258962 from Patent EP1572962.
JD355975 - Sequence 336999 from Patent EP1572962.
JD356063 - Sequence 337087 from Patent EP1572962.
JD324662 - Sequence 305686 from Patent EP1572962.
JD468003 - Sequence 449027 from Patent EP1572962.
JD245051 - Sequence 226075 from Patent EP1572962.
JD185604 - Sequence 166628 from Patent EP1572962.
JD058617 - Sequence 39641 from Patent EP1572962.
CR627435 - Homo sapiens mRNA; cDNA DKFZp781B2386 (from clone DKFZp781B2386).
JD133461 - Sequence 114485 from Patent EP1572962.
AK097331 - Homo sapiens cDNA FLJ40012 fis, clone STOMA2005782, weakly similar to Pseudouridylate synthase 7 homolog (EC 5.4.99.-).
BC033621 - Homo sapiens pseudouridylate synthase 7 homolog (S. cerevisiae)-like, mRNA (cDNA clone MGC:40389 IMAGE:5195232), complete cds.
AK225903 - Homo sapiens mRNA for hypothetical protein LOC83448 variant, clone: FCC120B04.
AK092030 - Homo sapiens cDNA FLJ34711 fis, clone MESAN2003444, weakly similar to 39.1 KDA PROTEIN IN SURE-CYSC INTERGENIC REGION.
AX747323 - Sequence 848 from Patent EP1308459.
KJ899701 - Synthetic construct Homo sapiens clone ccsbBroadEn_09095 PUS7L gene, encodes complete protein.
KJ899702 - Synthetic construct Homo sapiens clone ccsbBroadEn_09096 PUS7L gene, encodes complete protein.
AB527251 - Synthetic construct DNA, clone: pF1KE0005, Homo sapiens PUS7L gene for pseudouridylate synthase 7 homolog (S. cerevisiae)-like, without stop codon, in Flexi system.
AM392971 - Synthetic construct Homo sapiens clone IMAGE:100001774 for hypothetical protein (PUS7L gene).
BC020781 - Homo sapiens pseudouridylate synthase 7 homolog (S. cerevisiae)-like, mRNA (cDNA clone IMAGE:4719235), partial cds.
BC018000 - Homo sapiens pseudouridylate synthase 7 homolog (S. cerevisiae)-like, mRNA (cDNA clone IMAGE:4254137).
CU686653 - Synthetic construct Homo sapiens gateway clone IMAGE:100022860 5' read PUS7L mRNA.
JD163317 - Sequence 144341 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: BX647494, ENST00000416848.1, ENST00000416848.2, ENST00000416848.3, ENST00000416848.4, ENST00000416848.5, PUS7L_HUMAN, Q05CU0, Q6AHZ3, Q6NUP2, Q9H0K6, uc001rnq.1, uc001rnq.2, uc001rnq.3, uc001rnq.4, uc001rnq.5, uc001rnq.6, uc001rnq.7
UCSC ID: ENST00000416848.6
RefSeq Accession: NM_001098615
Protein: Q9H0K6 (aka PUS7L_HUMAN)
CCDS: CCDS8743.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.