Human Gene TROAP (ENST00000257909.8) from GENCODE V44
  Description: Homo sapiens trophinin associated protein (TROAP), transcript variant 1, mRNA. (from RefSeq NM_005480)
Gencode Transcript: ENST00000257909.8
Gencode Gene: ENSG00000135451.13
Transcript (Including UTRs)
   Position: hg38 chr12:49,323,255-49,331,731 Size: 8,477 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg38 chr12:49,323,609-49,331,617 Size: 8,009 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:49,323,255-49,331,731)mRNA (may differ from genome)Protein (778 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TROAP_HUMAN
DESCRIPTION: RecName: Full=Tastin; AltName: Full=Trophinin-assisting protein; AltName: Full=Trophinin-associated protein;
FUNCTION: Could be involved with bystin and trophinin in a cell adhesion molecule complex that mediates an initial attachment of the blastocyst to uterine epithelial cells at the time of the embryo implantation.
SUBUNIT: Directly binds bystin, and indirectly trophinin.
INTERACTION: Q13895:BYSL; NbExp=9; IntAct=EBI-2349743, EBI-358049;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Strong expression at implantation sites. Was exclusively localized to the apical side of the syncytiotrophoblast. Also found in macrophages.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TROAP
Diseases sorted by gene-association score: ectopic pregnancy (7)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.91 RPKM in Testis
Total median expression: 54.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -27.50100-0.275 Picture PostScript Text
3' UTR -16.90114-0.148 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026133 - Tastin

ModBase Predicted Comparative 3D Structure on Q12815
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0007155 cell adhesion

Cellular Component:
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK315211 - Homo sapiens cDNA, FLJ96203.
AK296811 - Homo sapiens cDNA FLJ50566 complete cds, highly similar to Trophinin-associated protein.
AK309716 - Homo sapiens cDNA, FLJ99757.
AK310709 - Homo sapiens cDNA, FLJ17751.
U04810 - Human tastin mRNA, complete cds.
AK299302 - Homo sapiens cDNA FLJ50628 complete cds, highly similar to Trophinin-associated protein.
BC032583 - Homo sapiens trophinin associated protein (tastin), mRNA (cDNA clone MGC:45238 IMAGE:5498800), complete cds.
BC018627 - Homo sapiens trophinin associated protein (tastin), mRNA (cDNA clone IMAGE:4890020), with apparent retained intron.
JD471183 - Sequence 452207 from Patent EP1572962.
BC011597 - Homo sapiens trophinin associated protein (tastin), mRNA (cDNA clone IMAGE:3347740), complete cds.
DQ890907 - Synthetic construct clone IMAGE:100003537; FLH166979.01X; RZPDo839F0988D trophinin associated protein (tastin) (TROAP) gene, encodes complete protein.
DQ893560 - Synthetic construct Homo sapiens clone IMAGE:100006190; FLH263556.01X; RZPDo839F1288D trophinin associated protein (tastin) (TROAP) gene, encodes complete protein.
EU176498 - Synthetic construct Homo sapiens clone IMAGE:100011352; FLH166978.01L; RZPDo839A04253D trophinin associated protein (tastin) (TROAP) gene, encodes complete protein.
KJ892900 - Synthetic construct Homo sapiens clone ccsbBroadEn_02294 TROAP gene, encodes complete protein.
CU689430 - Synthetic construct Homo sapiens gateway clone IMAGE:100017813 5' read TROAP mRNA.
JD405370 - Sequence 386394 from Patent EP1572962.
JD455191 - Sequence 436215 from Patent EP1572962.
JD405370 - Sequence 386394 from Patent EP1572962.
JD455191 - Sequence 436215 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000257909.1, ENST00000257909.2, ENST00000257909.3, ENST00000257909.4, ENST00000257909.5, ENST00000257909.6, ENST00000257909.7, NM_005480, Q12815, Q6PJU7, Q8N5B2, TROAP_HUMAN, uc001rtx.1, uc001rtx.2, uc001rtx.3, uc001rtx.4, uc001rtx.5, uc001rtx.6
UCSC ID: ENST00000257909.8
RefSeq Accession: NM_005480
Protein: Q12815 (aka TROAP_HUMAN or TAST_HUMAN)
CCDS: CCDS8784.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.