Human Gene RNF34 (ENST00000392465.7) from GENCODE V44
Description: Homo sapiens ring finger protein 34 (RNF34), transcript variant 1, mRNA. (from RefSeq NM_194271) RefSeq Summary (NM_194271): The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]. Gencode Transcript: ENST00000392465.7 Gencode Gene: ENSG00000170633.17 Transcript (Including UTRs) Position: hg38 chr12:121,400,119-121,424,348 Size: 24,230 Total Exon Count: 7 Strand: + Coding Region Position: hg38 chr12:121,402,799-121,423,576 Size: 20,778 Coding Exon Count: 6
ID:RNF34_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase RNF34; EC=6.3.2.-; AltName: Full=Caspase regulator CARP1; AltName: Full=Caspases-8 and -10-associated RING finger protein 1; Short=CARP-1; AltName: Full=FYVE-RING finger protein Momo; AltName: Full=Human RING finger homologous to inhibitor of apoptosis protein; Short=hRFI; AltName: Full=RING finger protein 34; AltName: Full=RING finger protein RIFF; FUNCTION: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with DNAJA3 (Potential). Interacts with CASP8 and CASP10. SUBCELLULAR LOCATION: Nucleus speckle. Endomembrane system; Peripheral membrane protein. TISSUE SPECIFICITY: Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. PTM: Auto-ubiquitinated (in vitro) (By similarity). PTM: Proteolytically cleaved by caspases upon induction of apoptosis by TNF. SIMILARITY: Contains 1 FYVE-type zinc finger. SIMILARITY: Contains 1 RING-type zinc finger. SIMILARITY: Contains 2 SAP domains. CAUTION: Was isolated as potential interaction partner of the mitochondrial protein DNAJA3, but the 2 proteins are not in the same cellular compartments. SEQUENCE CAUTION: Sequence=BAH14169.1; Type=Miscellaneous discrepancy; Note=Intron retention;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q969K3
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006511 ubiquitin-dependent protein catabolic process GO:0006915 apoptotic process GO:0016567 protein ubiquitination GO:0035872 nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process GO:0070417 cellular response to cold GO:0070936 protein K48-linked ubiquitination GO:1901797 negative regulation of signal transduction by p53 class mediator GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:2000374 regulation of oxygen metabolic process GO:2001271 negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
AF306709 - Homo sapiens RING finger protein RIFF mRNA, complete cds. AK225285 - Homo sapiens mRNA for ring finger protein 34 isoform 2 variant, clone: HEP00326. AK025439 - Homo sapiens cDNA: FLJ21786 fis, clone HEP00326. AK304366 - Homo sapiens cDNA FLJ52837 complete cds, highly similar to RING finger protein 34. JD473182 - Sequence 454206 from Patent EP1572962. JD078736 - Sequence 59760 from Patent EP1572962. JD200619 - Sequence 181643 from Patent EP1572962. BC039029 - Homo sapiens ring finger protein 34, mRNA (cDNA clone IMAGE:5176937), with apparent retained intron. BC046174 - Homo sapiens ring finger protein 34, mRNA (cDNA clone IMAGE:4394489), partial cds. BC007826 - Homo sapiens ring finger protein 34, mRNA (cDNA clone MGC:14144 IMAGE:3347885), complete cds. CU675815 - Synthetic construct Homo sapiens gateway clone IMAGE:100019124 5' read RNF34 mRNA. CR457342 - Homo sapiens full open reading frame cDNA clone RZPDo834F0713D for gene RNF34, ring finger protein 34; complete cds, incl. stopcodon. AY098934 - Homo sapiens FYVE-RING finger protein MOMO mRNA, partial cds. BT007283 - Homo sapiens ring finger protein 34 mRNA, complete cds. AB084914 - Homo sapiens hRFI mRNA for hypothetical protein, complete cds. KJ894793 - Synthetic construct Homo sapiens clone ccsbBroadEn_04187 RNF34 gene, encodes complete protein. AK130355 - Homo sapiens cDNA FLJ26845 fis, clone PRS07831. AK096994 - Homo sapiens cDNA FLJ39675 fis, clone SMINT2009529. AK024957 - Homo sapiens cDNA: FLJ21304 fis, clone COL02111. JD513324 - Sequence 494348 from Patent EP1572962. JD316637 - Sequence 297661 from Patent EP1572962. JD348735 - Sequence 329759 from Patent EP1572962. JD463462 - Sequence 444486 from Patent EP1572962. JD461421 - Sequence 442445 from Patent EP1572962. JD137069 - Sequence 118093 from Patent EP1572962. JD245198 - Sequence 226222 from Patent EP1572962. JD477632 - Sequence 458656 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q969K3 (Reactome details) participates in the following event(s):
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-983147 Release of E3 from polyubiquitinated substrate R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-6804441 RNF34 or RFFL ubiquitinates phosphorylated TP53 R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-6804757 Regulation of TP53 Degradation R-HSA-1280218 Adaptive Immune System R-HSA-6806003 Regulation of TP53 Expression and Degradation R-HSA-168256 Immune System R-HSA-5633007 Regulation of TP53 Activity R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-212436 Generic Transcription Pathway R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)