Human Gene TSSK4 (ENST00000287913.10) from GENCODE V44
  Description: Homo sapiens testis specific serine kinase 4 (TSSK4), transcript variant 2, mRNA. (from RefSeq NM_174944)
RefSeq Summary (NM_174944): This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010].
Gencode Transcript: ENST00000287913.10
Gencode Gene: ENSG00000139908.15
Transcript (Including UTRs)
   Position: hg38 chr14:24,205,756-24,208,248 Size: 2,493 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr14:24,205,924-24,208,146 Size: 2,223 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:24,205,756-24,208,248)mRNA (may differ from genome)Protein (328 aa)
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OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TSSK4_HUMAN
DESCRIPTION: RecName: Full=Testis-specific serine/threonine-protein kinase 4; Short=TSK-4; Short=TSSK-4; Short=Testis-specific kinase 4; EC=2.7.11.1; AltName: Full=Serine/threonine-protein kinase 22E;
FUNCTION: May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by phosphorylation on Thr-197, potentially by autophosphorylation (By similarity).
INTERACTION: P16220:CREB1; NbExp=5; IntAct=EBI-1202583, EBI-711855;
TISSUE SPECIFICITY: Expressed only in the testis.
PTM: Autophosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TSSK4
Diseases sorted by gene-association score: oligospermia (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 28.83 RPKM in Testis
Total median expression: 80.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -46.20168-0.275 Picture PostScript Text
3' UTR -18.90102-0.185 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain

ModBase Predicted Comparative 3D Structure on Q6SA08
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006468 protein phosphorylation
GO:0007275 multicellular organism development
GO:0007283 spermatogenesis
GO:0016310 phosphorylation
GO:0030154 cell differentiation
GO:0032793 positive regulation of CREB transcription factor activity
GO:0035556 intracellular signal transduction

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC029918 - Homo sapiens testis-specific serine kinase 4, mRNA (cDNA clone IMAGE:5169979), with apparent retained intron.
AK131042 - Homo sapiens cDNA FLJ29008 fis, clone TST06032.
BC111088 - Homo sapiens testis-specific serine kinase 4, mRNA (cDNA clone MGC:133264 IMAGE:40036837), complete cds.
AY461663 - Homo sapiens testis-specific serine kinase 4 mRNA, complete cds.
AB528011 - Synthetic construct DNA, clone: pF1KE0753, Homo sapiens TSSK4 gene for testis-specific serine kinase 4, without stop codon, in Flexi system.
AF542390 - Homo sapiens AT03158-like protein mRNA, complete cds.
KJ904108 - Synthetic construct Homo sapiens clone ccsbBroadEn_13502 TSSK4 gene, encodes complete protein.
KR711551 - Synthetic construct Homo sapiens clone CCSBHm_00025926 TSSK4 (TSSK4) mRNA, encodes complete protein.
KR711552 - Synthetic construct Homo sapiens clone CCSBHm_00025927 TSSK4 (TSSK4) mRNA, encodes complete protein.
JD559039 - Sequence 540063 from Patent EP1572962.
JD148029 - Sequence 129053 from Patent EP1572962.
JD057908 - Sequence 38932 from Patent EP1572962.
JD355723 - Sequence 336747 from Patent EP1572962.
JD343314 - Sequence 324338 from Patent EP1572962.
JD205382 - Sequence 186406 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: C14orf20, ENST00000287913.1, ENST00000287913.2, ENST00000287913.3, ENST00000287913.4, ENST00000287913.5, ENST00000287913.6, ENST00000287913.7, ENST00000287913.8, ENST00000287913.9, NM_174944, Q2TA60, Q6SA08, Q6ZNM2, STK22E, TSSK4_HUMAN, TSSK5, uc001wng.1, uc001wng.2, uc001wng.3, uc001wng.4
UCSC ID: ENST00000287913.10
RefSeq Accession: NM_174944
Protein: Q6SA08 (aka TSSK4_HUMAN)
CCDS: CCDS9618.1, CCDS53890.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.