Human Gene POLE2 (ENST00000216367.10) from GENCODE V44
Description: Homo sapiens DNA polymerase epsilon 2, accessory subunit (POLE2), transcript variant 1, mRNA. (from RefSeq NM_002692) RefSeq Summary (NM_002692): DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017]. Gencode Transcript: ENST00000216367.10 Gencode Gene: ENSG00000100479.14 Transcript (Including UTRs) Position: hg38 chr14:49,643,555-49,688,214 Size: 44,660 Total Exon Count: 19 Strand: - Coding Region Position: hg38 chr14:49,643,652-49,688,203 Size: 44,552 Coding Exon Count: 19
ID:DPOE2_HUMAN DESCRIPTION: RecName: Full=DNA polymerase epsilon subunit 2; EC=2.7.7.7; AltName: Full=DNA polymerase II subunit 2; AltName: Full=DNA polymerase epsilon subunit B; FUNCTION: Participates in DNA repair and in chromosomal DNA replication. CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). SUBUNIT: Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis. SIMILARITY: Belongs to the DNA polymerase epsilon subunit B family. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/pole2/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P56282
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK293163 - Homo sapiens cDNA FLJ58128 complete cds, highly similar to DNA polymerase epsilon subunit 2 (EC 2.7.7.7). AF025840 - Homo sapiens DNA polymerase epsilon subunit B (DPE2) mRNA, complete cds. AF036899 - Homo sapiens DNA polymerase epsilon small subunit (POLE2) mRNA, complete cds. BX248064 - human full-length cDNA clone CS0DJ011YD14 of T cells (Jurkat cell line) of Homo sapiens (human). BC126218 - Homo sapiens polymerase (DNA directed), epsilon 2 (p59 subunit), mRNA (cDNA clone MGC:161496 IMAGE:8991934), complete cds. BC126220 - Homo sapiens polymerase (DNA directed), epsilon 2 (p59 subunit), mRNA (cDNA clone MGC:161498 IMAGE:8991936), complete cds. KJ891842 - Synthetic construct Homo sapiens clone ccsbBroadEn_01236 POLE2 gene, encodes complete protein. AK314963 - Homo sapiens cDNA, FLJ95878. HQ258453 - Synthetic construct Homo sapiens clone IMAGE:100072882 polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2) gene, encodes complete protein. AB590929 - Synthetic construct DNA, clone: pFN21AB9939, Homo sapiens POLE2 gene for polymerase (DNA directed), epsilon 2, without stop codon, in Flexi system. BC112962 - Homo sapiens polymerase (DNA directed), epsilon 2 (p59 subunit), mRNA (cDNA clone MGC:132403 IMAGE:7481939), complete cds. KJ897354 - Synthetic construct Homo sapiens clone ccsbBroadEn_06748 POLE2 gene, encodes complete protein.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00230 - Purine metabolism hsa00240 - Pyrimidine metabolism hsa01100 - Metabolic pathways hsa03030 - DNA replication hsa03410 - Base excision repair hsa03420 - Nucleotide excision repair
Reactome (by CSHL, EBI, and GO)
Protein P56282 (Reactome details) participates in the following event(s):
R-HSA-68960 DNA polymerase epsilon binds at the origin R-HSA-68913 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the origin R-HSA-174425 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere R-HSA-68914 DNA polymerase alpha:primase binds at the origin R-HSA-5653838 POLD,POLE binds deISGylated PCNA after TLS R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA R-HSA-110364 PCNA:POLD,POLE:RPA:RFC and FEN1 bind APEX1 R-HSA-5651992 PCNA-containing replication complex binds damaged dsDNA R-HSA-5651809 LIG1, APEX1 and PCNA:POLD,POLE:RPA:RFC dissociate from repaired DNA R-HSA-5653840 POLD,POLE complete replication of damaged DNA after TLS R-HSA-5690213 DNA polymerases delta, epsilon or kappa bind the GG-NER site R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-110368 POLD,POLE-mediated DNA strand displacement synthesis R-HSA-110371 LIG1 binds APEX1 and PCNA at SSB R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA R-HSA-5651805 LIG1 bound to APEX1 and PCNA ligates SSB R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5690997 Ligation of newly synthesized repair patch to incised DNA in GG-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-5690988 3'-incision of DNA by ERCC5 (XPG) in GG-NER R-HSA-5691001 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in GG-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-68962 Activation of the pre-replicative complex R-HSA-68952 DNA replication initiation R-HSA-174430 Telomere C-strand synthesis initiation R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-69239 Synthesis of DNA R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair R-HSA-5696400 Dual Incision in GG-NER R-HSA-6782135 Dual incision in TC-NER R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-180786 Extension of Telomeres R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-HSA-5696399 Global Genome Nucleotide Excision Repair (GG-NER) R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-73894 DNA Repair R-HSA-69278 Cell Cycle (Mitotic) R-HSA-157579 Telomere Maintenance R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-73933 Resolution of Abasic Sites (AP sites) R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-5696398 Nucleotide Excision Repair R-HSA-1640170 Cell Cycle R-HSA-73886 Chromosome Maintenance R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-73884 Base Excision Repair R-HSA-5693538 Homology Directed Repair R-HSA-5693532 DNA Double-Strand Break Repair
US Server
