Human Gene EHD4 (ENST00000220325.9) from GENCODE V44
  Description: Homo sapiens EH domain containing 4 (EHD4), mRNA. (from RefSeq NM_139265)
Gencode Transcript: ENST00000220325.9
Gencode Gene: ENSG00000103966.11
Transcript (Including UTRs)
   Position: hg38 chr15:41,895,933-41,972,557 Size: 76,625 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr15:41,900,645-41,972,494 Size: 71,850 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:41,895,933-41,972,557)mRNA (may differ from genome)Protein (541 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: EHD4_HUMAN
DESCRIPTION: RecName: Full=EH domain-containing protein 4; AltName: Full=Hepatocellular carcinoma-associated protein 10/11; AltName: Full=PAST homolog 4;
FUNCTION: Plays a role in early endosomal transport.
SUBUNIT: Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.
SUBCELLULAR LOCATION: Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Highly expressed in pancreas and heart.
DOMAIN: The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins (By similarity).
SIMILARITY: Contains 1 EF-hand domain.
SIMILARITY: Contains 1 EH domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 43.22 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 545.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.2063-0.337 Picture PostScript Text
3' UTR -1641.804712-0.348 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001401 - Dynamin_GTPase
IPR011992 - EF-hand-like_dom
IPR018247 - EF_Hand_1_Ca_BS
IPR018249 - EF_HAND_2
IPR000261 - EPS15_homology

Pfam Domains:
PF00350 - Dynamin family

ModBase Predicted Comparative 3D Structure on Q9H223
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0045296 cadherin binding
GO:0046872 metal ion binding

Biological Process:
GO:0006907 pinocytosis
GO:0030100 regulation of endocytosis
GO:0032456 endocytic recycling
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051260 protein homooligomerization
GO:0071363 cellular response to growth factor stimulus

Cellular Component:
GO:0005634 nucleus
GO:0005768 endosome
GO:0005783 endoplasmic reticulum
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031901 early endosome membrane
GO:0048471 perinuclear region of cytoplasm
GO:0055038 recycling endosome membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF205850 - JP 2014500723-A/13353: Polycomb-Associated Non-Coding RNAs.
MA441427 - JP 2018138019-A/13353: Polycomb-Associated Non-Coding RNAs.
BC077716 - Homo sapiens cDNA clone IMAGE:5020413, containing frame-shift errors.
AF454953 - Homo sapiens EH domain-containing protein-4 mRNA, complete cds.
BC006287 - Homo sapiens EH-domain containing 4, mRNA (cDNA clone MGC:10536 IMAGE:3958645), complete cds.
BC051823 - Homo sapiens EH-domain containing 4, mRNA (cDNA clone MGC:60080 IMAGE:4944876), complete cds.
AK024902 - Homo sapiens cDNA: FLJ21249 fis, clone COL01244, highly similar to AF181265 Homo sapiens EH domain containing 4 (EHD4) mRNA.
AF181265 - Homo sapiens EH domain containing 4 (EHD4) mRNA, partial cds.
AF323924 - Homo sapiens hepatocellular carcinoma-associated protein HCA11 mRNA, complete cds.
AF307137 - Homo sapiens EH domain-containing protein FKSG7 (FKSG7) mRNA, complete cds.
AK292634 - Homo sapiens cDNA FLJ77391 complete cds, highly similar to Homo sapiens EH-domain containing 4 (EHD4), mRNA.
KJ906140 - Synthetic construct Homo sapiens clone ccsbBroadEn_15810 EHD4 gene, encodes complete protein.
DQ892745 - Synthetic construct clone IMAGE:100005375; FLH189305.01X; RZPDo839D0974D EH-domain containing 4 (EHD4) gene, encodes complete protein.
DQ895991 - Synthetic construct Homo sapiens clone IMAGE:100010451; FLH189301.01L; RZPDo839D0964D EH-domain containing 4 (EHD4) gene, encodes complete protein.
CU690850 - Synthetic construct Homo sapiens gateway clone IMAGE:100021441 5' read EHD4 mRNA.
AK074621 - Homo sapiens cDNA FLJ90140 fis, clone HEMBB1001048.
AL137436 - Homo sapiens mRNA; cDNA DKFZp761H0824 (from clone DKFZp761H0824).
JD264725 - Sequence 245749 from Patent EP1572962.
JD415668 - Sequence 396692 from Patent EP1572962.
JD499756 - Sequence 480780 from Patent EP1572962.
LF348863 - JP 2014500723-A/156366: Polycomb-Associated Non-Coding RNAs.
MA584440 - JP 2018138019-A/156366: Polycomb-Associated Non-Coding RNAs.
AF086163 - Homo sapiens full length insert cDNA clone ZB78B06.
JD062250 - Sequence 43274 from Patent EP1572962.
JD243750 - Sequence 224774 from Patent EP1572962.
JD042301 - Sequence 23325 from Patent EP1572962.
JD411312 - Sequence 392336 from Patent EP1572962.
JD269078 - Sequence 250102 from Patent EP1572962.
JD564989 - Sequence 546013 from Patent EP1572962.
JD244355 - Sequence 225379 from Patent EP1572962.
JD104707 - Sequence 85731 from Patent EP1572962.
JD206978 - Sequence 188002 from Patent EP1572962.
JD368258 - Sequence 349282 from Patent EP1572962.
JD534974 - Sequence 515998 from Patent EP1572962.
JD545342 - Sequence 526366 from Patent EP1572962.
JD438053 - Sequence 419077 from Patent EP1572962.
JD330646 - Sequence 311670 from Patent EP1572962.
LF348864 - JP 2014500723-A/156367: Polycomb-Associated Non-Coding RNAs.
MA584441 - JP 2018138019-A/156367: Polycomb-Associated Non-Coding RNAs.
JD078219 - Sequence 59243 from Patent EP1572962.
JD212544 - Sequence 193568 from Patent EP1572962.
JD493140 - Sequence 474164 from Patent EP1572962.
LF348865 - JP 2014500723-A/156368: Polycomb-Associated Non-Coding RNAs.
MA584442 - JP 2018138019-A/156368: Polycomb-Associated Non-Coding RNAs.
JD474140 - Sequence 455164 from Patent EP1572962.
JD044706 - Sequence 25730 from Patent EP1572962.
JD218938 - Sequence 199962 from Patent EP1572962.
LF348866 - JP 2014500723-A/156369: Polycomb-Associated Non-Coding RNAs.
MA584443 - JP 2018138019-A/156369: Polycomb-Associated Non-Coding RNAs.
LF348867 - JP 2014500723-A/156370: Polycomb-Associated Non-Coding RNAs.
MA584444 - JP 2018138019-A/156370: Polycomb-Associated Non-Coding RNAs.
LF348873 - JP 2014500723-A/156376: Polycomb-Associated Non-Coding RNAs.
MA584450 - JP 2018138019-A/156376: Polycomb-Associated Non-Coding RNAs.
LF348877 - JP 2014500723-A/156380: Polycomb-Associated Non-Coding RNAs.
MA584454 - JP 2018138019-A/156380: Polycomb-Associated Non-Coding RNAs.
JD204150 - Sequence 185174 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04144 - Endocytosis

-  Other Names for This Gene
  Alternate Gene Symbols: EHD4_HUMAN, ENST00000220325.1, ENST00000220325.2, ENST00000220325.3, ENST00000220325.4, ENST00000220325.5, ENST00000220325.6, ENST00000220325.7, ENST00000220325.8, FKSG7, HCA10, HCA11, NM_139265, PAST4, Q9H223, Q9HAR1, Q9NZN2, uc001zot.1, uc001zot.2, uc001zot.3, uc001zot.4, uc001zot.5
UCSC ID: ENST00000220325.9
RefSeq Accession: NM_139265
Protein: Q9H223 (aka EHD4_HUMAN)
CCDS: CCDS10081.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.