Human Gene HMOX2 (ENST00000570646.6) from GENCODE V44
  Description: Homo sapiens heme oxygenase 2 (HMOX2), transcript variant 3, mRNA. (from RefSeq NM_002134)
RefSeq Summary (NM_002134): Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013].
Gencode Transcript: ENST00000570646.6
Gencode Gene: ENSG00000103415.12
Transcript (Including UTRs)
   Position: hg38 chr16:4,476,398-4,510,347 Size: 33,950 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr16:4,505,525-4,509,756 Size: 4,232 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:4,476,398-4,510,347)mRNA (may differ from genome)Protein (316 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HMOX2_HUMAN
DESCRIPTION: RecName: Full=Heme oxygenase 2; Short=HO-2; EC=1.14.99.3;
FUNCTION: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
CATALYTIC ACTIVITY: Heme + 3 AH(2) + 3 O(2) = biliverdin + Fe(2+) + CO + 3 A + 3 H(2)O.
SUBCELLULAR LOCATION: Microsome. Endoplasmic reticulum.
INDUCTION: Heme oxygenase 2 activity is non-inducible.
SIMILARITY: Belongs to the heme oxygenase family.
SIMILARITY: Contains 2 HRM (heme regulatory motif) repeats.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/hmox2/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: HMOX2
Diseases sorted by gene-association score: arrhythmogenic right ventricular dysplasia 4 (11), diabetes mellitus, insulin-dependent, 7 (9), glass syndrome (9), nodular malignant melanoma (9), neonatal jaundice (9), carbamoylphosphate synthetase i deficiency (7), hypertrophic pyloric stenosis (7), congenital aphakia (6), type 1 diabetes mellitus 10 (6), neurodegeneration with brain iron accumulation 4 (6), pyloric stenosis (6), arrhythmogenic right ventricular dysplasia 1 (5), retinitis pigmentosa 26 (5), cardiomyopathy, dilated, 1d (5), split-hand/foot malformation 5 (4), cleft palate, isolated (2), hirschsprung disease 1 (2), preeclampsia/eclampsia 1 (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 58.25 RPKM in Testis
Total median expression: 908.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -52.10131-0.398 Picture PostScript Text
3' UTR -222.10591-0.376 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002051 - Haem_Oase
IPR016053 - Haem_Oase-like
IPR016084 - Haem_Oase-like_multi-hlx
IPR018207 - Haem_oxygenase_CS

Pfam Domains:
PF01126 - Heme oxygenase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2Q32 - X-ray MuPIT 2QPP - X-ray MuPIT 2RGZ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P30519
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004392 heme oxygenase (decyclizing) activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0020037 heme binding
GO:0046872 metal ion binding

Biological Process:
GO:0001666 response to hypoxia
GO:0006788 heme oxidation
GO:0006879 cellular iron ion homeostasis
GO:0006979 response to oxidative stress
GO:0042167 heme catabolic process
GO:0043312 neutrophil degranulation
GO:0055072 iron ion homeostasis
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0035579 specific granule membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  AK313660 - Homo sapiens cDNA, FLJ94240.
BC002396 - Homo sapiens heme oxygenase (decycling) 2, mRNA (cDNA clone MGC:8455 IMAGE:2821444), complete cds.
S34389 - heme oxygenase-2 [human, kidney, mRNA, 1627 nt].
AK222629 - Homo sapiens mRNA for heme oxygenase (decyclizing) 2 variant, clone: CBL04619.
JD472675 - Sequence 453699 from Patent EP1572962.
AK093388 - Homo sapiens cDNA FLJ36069 fis, clone TESTI2019406, highly similar to HEME OXYGENASE 2 (EC 1.14.99.3).
KJ891365 - Synthetic construct Homo sapiens clone ccsbBroadEn_00759 HMOX2 gene, encodes complete protein.
CR456760 - Homo sapiens full open reading frame cDNA clone RZPDo834C094D for gene HMOX2, heme oxygenase (decycling) 2; complete cds, incl. stopcodon.
AB587376 - Synthetic construct DNA, clone: pF1KB5034, Homo sapiens HMOX2 gene for heme oxygenase (decycling) 2, without stop codon, in Flexi system.
CR541839 - Homo sapiens full open reading frame cDNA clone RZPDo834A1032D for gene HMOX2, heme oxygenase (decycling) 2; complete cds, without stopcodon.
BT019788 - Homo sapiens heme oxygenase (decycling) 2 mRNA, complete cds.
EU831544 - Synthetic construct Homo sapiens clone HAIB:100066573; DKFZo004E0918 heme oxygenase (decycling) 2 protein (HMOX2) gene, encodes complete protein.
EU831457 - Synthetic construct Homo sapiens clone HAIB:100066486; DKFZo008E0917 heme oxygenase (decycling) 2 protein (HMOX2) gene, encodes complete protein.
D21243 - Homo sapiens mRNA for heme oxygenase-2, complete cds.
AF051306 - Homo sapiens heme oxygenase 2 mRNA, partial cds.
JD054162 - Sequence 35186 from Patent EP1572962.
JD369513 - Sequence 350537 from Patent EP1572962.
JD355987 - Sequence 337011 from Patent EP1572962.
JD159258 - Sequence 140282 from Patent EP1572962.
JD251241 - Sequence 232265 from Patent EP1572962.
JD323969 - Sequence 304993 from Patent EP1572962.
JD393317 - Sequence 374341 from Patent EP1572962.
JD401859 - Sequence 382883 from Patent EP1572962.
JD283142 - Sequence 264166 from Patent EP1572962.
JD117850 - Sequence 98874 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00860 - Porphyrin and chlorophyll metabolism

BioCyc Knowledge Library
PWY-5874 - heme degradation I

BioCarta from NCI Cancer Genome Anatomy Project
h_arenrf2Pathway - Oxidative Stress Induced Gene Expression Via Nrf2

Reactome (by CSHL, EBI, and GO)

Protein P30519 (Reactome details) participates in the following event(s):

R-HSA-6799350 Exocytosis of specific granule membrane proteins
R-HSA-189398 HMOX1,2 cleave heme
R-HSA-6798695 Neutrophil degranulation
R-HSA-189483 Heme degradation
R-HSA-917937 Iron uptake and transport
R-HSA-168249 Innate Immune System
R-HSA-189445 Metabolism of porphyrins
R-HSA-382551 Transport of small molecules
R-HSA-168256 Immune System
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8MT35, D3DUD5, ENST00000570646.1, ENST00000570646.2, ENST00000570646.3, ENST00000570646.4, ENST00000570646.5, HMOX2_HUMAN, HO2, NM_002134, O60605, P30519, uc002cwq.1, uc002cwq.2, uc002cwq.3, uc002cwq.4, uc002cwq.5, uc002cwq.6
UCSC ID: ENST00000570646.6
RefSeq Accession: NM_002134
Protein: P30519 (aka HMOX2_HUMAN or HO2_HUMAN)
CCDS: CCDS10517.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.