Human Gene AMFR (ENST00000290649.10) from GENCODE V44
Description: Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA. (from RefSeq NM_001323512) RefSeq Summary (NM_001144): This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]. Gencode Transcript: ENST00000290649.10 Gencode Gene: ENSG00000159461.15 Transcript (Including UTRs) Position: hg38 chr16:56,361,452-56,425,545 Size: 64,094 Total Exon Count: 14 Strand: - Coding Region Position: hg38 chr16:56,362,909-56,425,327 Size: 62,419 Coding Exon Count: 14
ID:AMFR_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase AMFR; EC=6.3.2.-; AltName: Full=Autocrine motility factor receptor; Short=AMF receptor; AltName: Full=RING finger protein 45; AltName: Full=gp78; FUNCTION: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation (By similarity). Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. INTERACTION: P55072:VCP; NbExp=6; IntAct=EBI-1046379, EBI-355164; SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. DOMAIN: The VCP/p97-interacting motif (VIM) is sufficient for binding VCP/p97 to form a complex capable of transfering VCP/p97 from the cytosol to microsomes. SIMILARITY: Contains 1 CUE domain. SIMILARITY: Contains 1 RING-type zinc finger. SEQUENCE CAUTION: Sequence=AAA36671.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors; Sequence=AAA79362.1; Type=Frameshift; Positions=355, 388, 411, 487, 537, 583, 632;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UKV5
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000209 protein polyubiquitination GO:0006457 protein folding GO:0006511 ubiquitin-dependent protein catabolic process GO:0007165 signal transduction GO:0007568 aging GO:0007611 learning or memory GO:0016567 protein ubiquitination GO:0030433 ER-associated ubiquitin-dependent protein catabolic process GO:0030968 endoplasmic reticulum unfolded protein response GO:0032092 positive regulation of protein binding GO:0051259 protein oligomerization GO:0051865 protein autoubiquitination GO:0070936 protein K48-linked ubiquitination GO:1904380 endoplasmic reticulum mannose trimming
US Server
Sequence and Links to Tools and Databases 
Microarray Expression Data 
