Human Gene ALDOC (ENST00000226253.9) from GENCODE V44
  Description: Homo sapiens aldolase, fructose-bisphosphate C (ALDOC), mRNA. (from RefSeq NM_005165)
RefSeq Summary (NM_005165): This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000226253.9
Gencode Gene: ENSG00000109107.14
Transcript (Including UTRs)
   Position: hg38 chr17:28,573,120-28,576,895 Size: 3,776 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg38 chr17:28,573,526-28,575,532 Size: 2,007 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:28,573,120-28,576,895)mRNA (may differ from genome)Protein (364 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ALDOC_HUMAN
DESCRIPTION: RecName: Full=Fructose-bisphosphate aldolase C; EC=4.1.2.13; AltName: Full=Brain-type aldolase;
CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=10.7 uM for fructose 1,6-bisphosphate; KM=16 mM for fructose 1-phosphate;
PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 4/4.
SUBUNIT: Homotetramer. Interacts with ATP6V1E1. May interact with PLD2.
MISCELLANEOUS: In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.
SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase family.
SEQUENCE CAUTION: Sequence=AAI03761.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D003024 Clozapine
  • D016572 Cyclosporine
  • D004997 Ethinyl Estradiol
  • D010100 Oxygen
  • D013749 Tetrachlorodibenzodioxin
  • D014635 Valproic Acid
  • C025205 1,10-phenanthroline
  • D015647 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • C054649 3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol
          more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 519.16 RPKM in Brain - Cerebellum
Total median expression: 4488.60 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.10107-0.375 Picture PostScript Text
3' UTR -125.80406-0.310 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000741 - Aldolase_I
IPR013785 - Aldolase_TIM

Pfam Domains:
PF00274 - Fructose-bisphosphate aldolase class-I

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1XFB - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P09972
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004332 fructose-bisphosphate aldolase activity
GO:0005515 protein binding
GO:0008092 cytoskeletal protein binding
GO:0016829 lyase activity

Biological Process:
GO:0006000 fructose metabolic process
GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0030388 fructose 1,6-bisphosphate metabolic process
GO:0030855 epithelial cell differentiation
GO:0043312 neutrophil degranulation
GO:0061621 canonical glycolysis

Cellular Component:
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0034774 secretory granule lumen
GO:0070062 extracellular exosome
GO:1904724 tertiary granule lumen
GO:1904813 ficolin-1-rich granule lumen


-  Descriptions from all associated GenBank mRNAs
  LF209214 - JP 2014500723-A/16717: Polycomb-Associated Non-Coding RNAs.
MA444791 - JP 2018138019-A/16717: Polycomb-Associated Non-Coding RNAs.
AK294157 - Homo sapiens cDNA FLJ52210 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC 4.1.2.13).
AF054987 - Homo sapiens clone 23831 aldolase C mRNA, complete cds.
BC003613 - Homo sapiens aldolase C, fructose-bisphosphate, mRNA (cDNA clone MGC:1449 IMAGE:2987869), complete cds.
BC065565 - Homo sapiens aldolase C, fructose-bisphosphate, mRNA (cDNA clone MGC:75098 IMAGE:6155379), complete cds.
BC103760 - Homo sapiens aldolase C, fructose-bisphosphate, mRNA (cDNA clone IMAGE:6139644), partial cds.
AK293623 - Homo sapiens cDNA FLJ52578 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC 4.1.2.13).
AK293693 - Homo sapiens cDNA FLJ52222 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC4.1.2.13).
BC106925 - Homo sapiens aldolase C, fructose-bisphosphate, mRNA (cDNA clone MGC:126080 IMAGE:40032967), complete cds.
BC106926 - Homo sapiens aldolase C, fructose-bisphosphate, mRNA (cDNA clone MGC:126081 IMAGE:40032968), complete cds.
AK294091 - Homo sapiens cDNA FLJ51814 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC 4.1.2.13).
AK296039 - Homo sapiens cDNA FLJ50972 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC 4.1.2.13).
AK296053 - Homo sapiens cDNA FLJ56450 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC 4.1.2.13).
CR541862 - Homo sapiens full open reading frame cDNA clone RZPDo834F0832D for gene ALDOC, aldolase C, fructose-bisphosphate; complete cds, incl. stopcodon.
GQ891338 - Homo sapiens clone HEL-S-109d epididymis secretory sperm binding protein mRNA, complete cds.
AK312281 - Homo sapiens cDNA, FLJ92580, Homo sapiens aldolase C, fructose-bisphosphate (ALDOC), mRNA.
BT007006 - Homo sapiens aldolase C, fructose-bisphosphate mRNA, complete cds.
KJ890660 - Synthetic construct Homo sapiens clone ccsbBroadEn_00054 ALDOC gene, encodes complete protein.
CR541881 - Homo sapiens full open reading frame cDNA clone RZPDo834A0533D for gene ALDOC, aldolase C, fructose-bisphosphate; complete cds, without stopcodon.
JD228529 - Sequence 209553 from Patent EP1572962.
JD271141 - Sequence 252165 from Patent EP1572962.
JD293837 - Sequence 274861 from Patent EP1572962.
JD521649 - Sequence 502673 from Patent EP1572962.
JD164668 - Sequence 145692 from Patent EP1572962.
JD393382 - Sequence 374406 from Patent EP1572962.
JD153881 - Sequence 134905 from Patent EP1572962.
JD117601 - Sequence 98625 from Patent EP1572962.
JD480345 - Sequence 461369 from Patent EP1572962.
JD355747 - Sequence 336771 from Patent EP1572962.
JD145533 - Sequence 126557 from Patent EP1572962.
JD421778 - Sequence 402802 from Patent EP1572962.
JD259839 - Sequence 240863 from Patent EP1572962.
JD403863 - Sequence 384887 from Patent EP1572962.
JD548578 - Sequence 529602 from Patent EP1572962.
JD380428 - Sequence 361452 from Patent EP1572962.
JD381380 - Sequence 362404 from Patent EP1572962.
JD060819 - Sequence 41843 from Patent EP1572962.
LF326848 - JP 2014500723-A/134351: Polycomb-Associated Non-Coding RNAs.
MA562425 - JP 2018138019-A/134351: Polycomb-Associated Non-Coding RNAs.
AK293453 - Homo sapiens cDNA FLJ52121 complete cds, highly similar to Fructose-bisphosphate aldolase C (EC4.1.2.13).
LF326847 - JP 2014500723-A/134350: Polycomb-Associated Non-Coding RNAs.
MA562424 - JP 2018138019-A/134350: Polycomb-Associated Non-Coding RNAs.
JD236566 - Sequence 217590 from Patent EP1572962.
JD021380 - Sequence 2404 from Patent EP1572962.
JD027743 - Sequence 8767 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00010 - Glycolysis / Gluconeogenesis
hsa00030 - Pentose phosphate pathway
hsa00051 - Fructose and mannose metabolism
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY66-373 - sucrose degradation
PWY66-399 - gluconeogenesis
PWY66-400 - glycolysis
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

Reactome (by CSHL, EBI, and GO)

Protein P09972 (Reactome details) participates in the following event(s):

R-HSA-6800434 Exocytosis of ficolin-rich granule lumen proteins
R-HSA-6798748 Exocytosis of secretory granule lumen proteins
R-HSA-6798745 Exocytosis of tertiary granule lumen proteins
R-HSA-71495 dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate <=> D-fructose 1,6-bisphosphate
R-HSA-71496 D-fructose 1,6-bisphosphate <=> dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
R-HSA-6798695 Neutrophil degranulation
R-HSA-168249 Innate Immune System
R-HSA-70263 Gluconeogenesis
R-HSA-70171 Glycolysis
R-HSA-168256 Immune System
R-HSA-70326 Glucose metabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ALDC, ALDOC_HUMAN, B2R5R3, ENST00000226253.1, ENST00000226253.2, ENST00000226253.3, ENST00000226253.4, ENST00000226253.5, ENST00000226253.6, ENST00000226253.7, ENST00000226253.8, NM_005165, P09972, Q3SYL3, Q6FH94, Q6P0L5, uc002hbp.1, uc002hbp.2, uc002hbp.3, uc002hbp.4, uc002hbp.5
UCSC ID: ENST00000226253.9
RefSeq Accession: NM_005165
Protein: P09972 (aka ALDOC_HUMAN or ALFC_HUMAN)
CCDS: CCDS11236.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.