Human Gene LASP1 (ENST00000318008.11) from GENCODE V44
  Description: Homo sapiens LIM and SH3 protein 1 (LASP1), transcript variant 1, mRNA. (from RefSeq NM_006148)
RefSeq Summary (NM_006148): This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012].
Gencode Transcript: ENST00000318008.11
Gencode Gene: ENSG00000002834.19
Transcript (Including UTRs)
   Position: hg38 chr17:38,870,058-38,921,770 Size: 51,713 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr17:38,870,190-38,918,778 Size: 48,589 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:38,870,058-38,921,770)mRNA (may differ from genome)Protein (261 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: LASP1_HUMAN
DESCRIPTION: RecName: Full=LIM and SH3 domain protein 1; Short=LASP-1; AltName: Full=Metastatic lymph node gene 50 protein; Short=MLN 50;
FUNCTION: Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
SUBUNIT: Interacts with F-actin (By similarity). Interacts with ANKRD54 (By similarity).
INTERACTION: Q99750:MDFI; NbExp=3; IntAct=EBI-742828, EBI-724076; Q15645:TRIP13; NbExp=3; IntAct=EBI-742828, EBI-358993;
SUBCELLULAR LOCATION: Cytoplasm, cell cortex (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Associated with the F-actin rich cortical cytoskeleton (By similarity).
SIMILARITY: Contains 1 LIM zinc-binding domain.
SIMILARITY: Contains 2 nebulin repeats.
SIMILARITY: Contains 1 SH3 domain.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/Lasp1ID203.html";

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: LASP1
Diseases sorted by gene-association score: breast cancer (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 66.26 RPKM in Cells - Cultured fibroblasts
Total median expression: 1241.18 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -48.80132-0.370 Picture PostScript Text
3' UTR -1153.402992-0.385 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000900 - Nebulin_35r-motif
IPR001452 - SH3_domain
IPR001781 - Znf_LIM

Pfam Domains:
PF00412 - LIM domain
PF00880 - Nebulin repeat
PF00018 - SH3 domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3I35 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q14847
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsemblWormBase 
Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence 
AlignmentAlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0005070 SH3/SH2 adaptor activity
GO:0005515 protein binding
GO:0015075 ion transmembrane transporter activity
GO:0045296 cadherin binding
GO:0046872 metal ion binding
GO:0051015 actin filament binding

Biological Process:
GO:0006811 ion transport
GO:0009967 positive regulation of signal transduction
GO:0034220 ion transmembrane transport

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0005938 cell cortex
GO:0030864 cortical actin cytoskeleton


-  Descriptions from all associated GenBank mRNAs
  AK307868 - Homo sapiens cDNA, FLJ97816.
AK294704 - Homo sapiens cDNA FLJ51834 complete cds, highly similar to LIM and SH3 domain protein 1.
AK296092 - Homo sapiens cDNA FLJ58065 complete cds, highly similar to LIM and SH3 domain protein 1.
AK309933 - Homo sapiens cDNA, FLJ99974.
AK295522 - Homo sapiens cDNA FLJ52195 complete cds, highly similar to LIM and SH3 domain protein 1.
BC007560 - Homo sapiens LIM and SH3 protein 1, mRNA (cDNA clone MGC:15487 IMAGE:2988698), complete cds.
X82456 - H.sapiens MLN50 mRNA.
BC012460 - Homo sapiens LIM and SH3 protein 1, mRNA (cDNA clone MGC:21215 IMAGE:4470852), complete cds.
DQ891539 - Synthetic construct clone IMAGE:100004169; FLH177847.01X; RZPDo839E08126D LIM and SH3 protein 1 (LASP1) gene, encodes complete protein.
DQ894728 - Synthetic construct Homo sapiens clone IMAGE:100009188; FLH177843.01L; RZPDo839E08125D LIM and SH3 protein 1 (LASP1) gene, encodes complete protein.
AB463833 - Synthetic construct DNA, clone: pF1KB6327, Homo sapiens LASP1 gene for LIM and SH3 protein 1, without stop codon, in Flexi system.
KJ897122 - Synthetic construct Homo sapiens clone ccsbBroadEn_06516 LASP1 gene, encodes complete protein.
CU675705 - Synthetic construct Homo sapiens gateway clone IMAGE:100018925 5' read LASP1 mRNA.
KJ901550 - Synthetic construct Homo sapiens clone ccsbBroadEn_10944 LASP1 gene, encodes complete protein.
AK289847 - Homo sapiens cDNA FLJ76601 complete cds, highly similar to Homo sapiens LIM and SH3 protein 1 (LASP1), mRNA.
JD405719 - Sequence 386743 from Patent EP1572962.
JD386296 - Sequence 367320 from Patent EP1572962.
JD135003 - Sequence 116027 from Patent EP1572962.
AK095958 - Homo sapiens cDNA FLJ38639 fis, clone HHDPC2003439.
JD300751 - Sequence 281775 from Patent EP1572962.
JD164925 - Sequence 145949 from Patent EP1572962.
JD261140 - Sequence 242164 from Patent EP1572962.
JD418909 - Sequence 399933 from Patent EP1572962.
JD413242 - Sequence 394266 from Patent EP1572962.
JD099765 - Sequence 80789 from Patent EP1572962.
JD428145 - Sequence 409169 from Patent EP1572962.
JD259246 - Sequence 240270 from Patent EP1572962.
JD052030 - Sequence 33054 from Patent EP1572962.
JD193623 - Sequence 174647 from Patent EP1572962.
JD215053 - Sequence 196077 from Patent EP1572962.
JD419325 - Sequence 400349 from Patent EP1572962.
JD260869 - Sequence 241893 from Patent EP1572962.
JD418455 - Sequence 399479 from Patent EP1572962.
JD130384 - Sequence 111408 from Patent EP1572962.
JD094819 - Sequence 75843 from Patent EP1572962.
JD249119 - Sequence 230143 from Patent EP1572962.
JD138691 - Sequence 119715 from Patent EP1572962.
JD242054 - Sequence 223078 from Patent EP1572962.
JD124602 - Sequence 105626 from Patent EP1572962.
JD056003 - Sequence 37027 from Patent EP1572962.
JD193927 - Sequence 174951 from Patent EP1572962.
JD422641 - Sequence 403665 from Patent EP1572962.
JD465731 - Sequence 446755 from Patent EP1572962.
JD323744 - Sequence 304768 from Patent EP1572962.
JD423642 - Sequence 404666 from Patent EP1572962.
JD058589 - Sequence 39613 from Patent EP1572962.
JD205620 - Sequence 186644 from Patent EP1572962.
JD219016 - Sequence 200040 from Patent EP1572962.
JD548430 - Sequence 529454 from Patent EP1572962.
JD217240 - Sequence 198264 from Patent EP1572962.
JD331399 - Sequence 312423 from Patent EP1572962.
JD078029 - Sequence 59053 from Patent EP1572962.
JD279375 - Sequence 260399 from Patent EP1572962.
JD222579 - Sequence 203603 from Patent EP1572962.
JD073907 - Sequence 54931 from Patent EP1572962.
JD163647 - Sequence 144671 from Patent EP1572962.
JD184646 - Sequence 165670 from Patent EP1572962.
JD319892 - Sequence 300916 from Patent EP1572962.
JD295961 - Sequence 276985 from Patent EP1572962.
JD239692 - Sequence 220716 from Patent EP1572962.
JD094608 - Sequence 75632 from Patent EP1572962.
JD375117 - Sequence 356141 from Patent EP1572962.
JD111243 - Sequence 92267 from Patent EP1572962.
JD565062 - Sequence 546086 from Patent EP1572962.
JD391547 - Sequence 372571 from Patent EP1572962.
JD137569 - Sequence 118593 from Patent EP1572962.
JD298019 - Sequence 279043 from Patent EP1572962.
JD521918 - Sequence 502942 from Patent EP1572962.
JD477989 - Sequence 459013 from Patent EP1572962.
JD465008 - Sequence 446032 from Patent EP1572962.
JD140970 - Sequence 121994 from Patent EP1572962.
JD221940 - Sequence 202964 from Patent EP1572962.
JD391213 - Sequence 372237 from Patent EP1572962.
JD561243 - Sequence 542267 from Patent EP1572962.
JD181199 - Sequence 162223 from Patent EP1572962.
JD042691 - Sequence 23715 from Patent EP1572962.
JD324387 - Sequence 305411 from Patent EP1572962.
JD064132 - Sequence 45156 from Patent EP1572962.
JD491815 - Sequence 472839 from Patent EP1572962.
BC007367 - Homo sapiens LIM and SH3 protein 1, mRNA (cDNA clone IMAGE:3632974), partial cds.
JD062258 - Sequence 43282 from Patent EP1572962.
JD098072 - Sequence 79096 from Patent EP1572962.
JD278532 - Sequence 259556 from Patent EP1572962.
JD466481 - Sequence 447505 from Patent EP1572962.
JD093361 - Sequence 74385 from Patent EP1572962.
JD383016 - Sequence 364040 from Patent EP1572962.
JD370126 - Sequence 351150 from Patent EP1572962.
JD060075 - Sequence 41099 from Patent EP1572962.
JD040641 - Sequence 21665 from Patent EP1572962.
JD549576 - Sequence 530600 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000318008.1, ENST00000318008.10, ENST00000318008.2, ENST00000318008.3, ENST00000318008.4, ENST00000318008.5, ENST00000318008.6, ENST00000318008.7, ENST00000318008.8, ENST00000318008.9, LASP1_HUMAN, MLN50, NM_006148, Q14847, Q96ED2, Q96IG0, uc002hra.1, uc002hra.2, uc002hra.3, uc002hra.4
UCSC ID: ENST00000318008.11
RefSeq Accession: NM_006148
Protein: Q14847 (aka LASP1_HUMAN or LAS1_HUMAN)
CCDS: CCDS11331.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.