Human Gene TRIM25 (ENST00000316881.9) Description and Page Index
Description: Homo sapiens tripartite motif containing 25 (TRIM25), mRNA. (from RefSeq NM_005082) RefSeq Summary (NM_005082): The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000316881.9 Gencode Gene: ENSG00000121060.18 Transcript (Including UTRs) Position: hg38 chr17:56,887,909-56,914,049 Size: 26,141 Total Exon Count: 9 Strand: - Coding Region Position: hg38 chr17:56,891,700-56,913,988 Size: 22,289 Coding Exon Count: 9
ID:TRI25_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin/ISG15 ligase TRIM25; EC=184.108.40.206; EC=6.3.2.n3; AltName: Full=Estrogen-responsive finger protein; AltName: Full=RING finger protein 147; AltName: Full=Tripartite motif-containing protein 25; AltName: Full=Ubiquitin/ISG15-conjugating enzyme TRIM25; AltName: Full=Zinc finger protein 147; FUNCTION: Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. CATALYTIC ACTIVITY: ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction. INTERACTION: P03496:NS (xeno); NbExp=3; IntAct=EBI-2341129, EBI-2547442; SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies. TISSUE SPECIFICITY: Ubiquitous. INDUCTION: By interferons. DOMAIN: The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity. DOMAIN: The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58. PTM: Auto-ISGylated. SIMILARITY: Contains 1 B30.2/SPRY domain. SIMILARITY: Contains 1 RING-type zinc finger. WEB RESOURCE: Name=Wikipedia; Note=TRIM25 entry; URL="http://en.wikipedia.org/wiki/TRIM25";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q14258
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0002376 immune system process GO:0006511 ubiquitin-dependent protein catabolic process GO:0006513 protein monoubiquitination GO:0016032 viral process GO:0016567 protein ubiquitination GO:0019985 translesion synthesis GO:0030433 ER-associated ubiquitin-dependent protein catabolic process GO:0032480 negative regulation of type I interferon production GO:0033280 response to vitamin D GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043627 response to estrogen GO:0045087 innate immune response GO:0046596 regulation of viral entry into host cell GO:0046597 negative regulation of viral entry into host cell GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity GO:0051092 positive regulation of NF-kappaB transcription factor activity GO:0051607 defense response to virus GO:0060333 interferon-gamma-mediated signaling pathway GO:1902186 regulation of viral release from host cell GO:1902187 negative regulation of viral release from host cell GO:1990830 cellular response to leukemia inhibitory factor