ID:THOC1_HUMAN DESCRIPTION: RecName: Full=THO complex subunit 1; Short=Tho1; AltName: Full=Nuclear matrix protein p84; Short=p84N5; AltName: Full=hTREX84; FUNCTION: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. FUNCTION: Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. SUBUNIT: Component of the THO complex, which is composed of THOC1, THOC2, THOC5, THOC6 and THOC7. Together with THOC3, ALYREF/THOC4 and DDX39B, THO forms the transcription/export (TREX) complex. Binds to the hypophosphorylated form of RB1. Interacts with THOC2, DDX39B and RNA polymerase II. SUBCELLULAR LOCATION: Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. INDUCTION: Up-regulated during cell proliferation. DOMAIN: An intact death domain is needed for apoptosis. PTM: Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. SIMILARITY: Contains 1 death domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96FV9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF210967 - JP 2014500723-A/18470: Polycomb-Associated Non-Coding RNAs. MA446544 - JP 2018138019-A/18470: Polycomb-Associated Non-Coding RNAs. AK225709 - Homo sapiens mRNA for THO complex subunit 1 variant, clone: SYN09250. AK225067 - Homo sapiens mRNA for THO complex subunit 1 variant, clone: CAE05835. L36529 - Human (clone N5-4) protein p84 mRNA, complete cds. BC010381 - Homo sapiens THO complex 1, mRNA (cDNA clone MGC:13557 IMAGE:4046908), complete cds. DQ892768 - Synthetic construct clone IMAGE:100005398; FLH189518.01X; RZPDo839G0174D THO complex 1 (THOC1) gene, encodes complete protein. AK314755 - Homo sapiens cDNA, FLJ95622, highly similar to Homo sapiens THO complex 1 (THOC1), mRNA. KJ898131 - Synthetic construct Homo sapiens clone ccsbBroadEn_07525 THOC1 gene, encodes complete protein. EU176747 - Synthetic construct Homo sapiens clone IMAGE:100011513; FLH189517.01L; RZPDo839C11255D THO complex 1 (THOC1) gene, encodes complete protein. AY573303 - Homo sapiens death domain-containing protein p84N5 short isoform (THOC1) mRNA, complete cds. CU679685 - Synthetic construct Homo sapiens gateway clone IMAGE:100018396 5' read THOC1 mRNA. AK055354 - Homo sapiens cDNA FLJ30792 fis, clone FEBRA2001004, highly similar to Human (clone N5-4) protein p84 mRNA. AK123530 - Homo sapiens cDNA FLJ41536 fis, clone BRTHA2017178, highly similar to Homo sapiens nuclear matrix protein p84 (P84). LF342667 - JP 2014500723-A/150170: Polycomb-Associated Non-Coding RNAs. MA578244 - JP 2018138019-A/150170: Polycomb-Associated Non-Coding RNAs. LF342665 - JP 2014500723-A/150168: Polycomb-Associated Non-Coding RNAs. MA578242 - JP 2018138019-A/150168: Polycomb-Associated Non-Coding RNAs. LF342664 - JP 2014500723-A/150167: Polycomb-Associated Non-Coding RNAs. MA578241 - JP 2018138019-A/150167: Polycomb-Associated Non-Coding RNAs. LF342662 - JP 2014500723-A/150165: Polycomb-Associated Non-Coding RNAs. MA578239 - JP 2018138019-A/150165: Polycomb-Associated Non-Coding RNAs. LF342656 - JP 2014500723-A/150159: Polycomb-Associated Non-Coding RNAs. MA578233 - JP 2018138019-A/150159: Polycomb-Associated Non-Coding RNAs. LF342648 - JP 2014500723-A/150151: Polycomb-Associated Non-Coding RNAs. MA578225 - JP 2018138019-A/150151: Polycomb-Associated Non-Coding RNAs. LF342647 - JP 2014500723-A/150150: Polycomb-Associated Non-Coding RNAs. MA578224 - JP 2018138019-A/150150: Polycomb-Associated Non-Coding RNAs. AY573302 - Homo sapiens death domain-containing protein p84N5 short isoform 2 (THOC1) mRNA, complete cds.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa03040 - Spliceosome
Reactome (by CSHL, EBI, and GO)
Protein Q96FV9 (Reactome details) participates in the following event(s):
R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-72185 mRNA polyadenylation R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-159101 NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B) R-HSA-72187 mRNA 3'-end processing R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-8953854 Metabolism of RNA R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Microarray Expression Data 
