Human Gene REV1 (ENST00000393445.7) from GENCODE V44
Description: Homo sapiens REV1 DNA directed polymerase (REV1), transcript variant 4, mRNA. (from RefSeq NM_001321455) RefSeq Summary (NM_001037872): This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]. Gencode Transcript: ENST00000393445.7 Gencode Gene: ENSG00000135945.10 Transcript (Including UTRs) Position: hg38 chr2:99,400,475-99,489,973 Size: 89,499 Total Exon Count: 23 Strand: - Coding Region Position: hg38 chr2:99,401,241-99,464,975 Size: 63,735 Coding Exon Count: 22
ID:REV1_HUMAN DESCRIPTION: RecName: Full=DNA repair protein REV1; EC=2.7.7.-; AltName: Full=Alpha integrin-binding protein 80; Short=AIBP80; AltName: Full=Rev1-like terminal deoxycytidyl transferase; FUNCTION: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. SUBUNIT: Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86. SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The C-terminal domain is necessary for protein interactions. SIMILARITY: Belongs to the DNA polymerase type-Y family. SIMILARITY: Contains 1 BRCT domain. SIMILARITY: Contains 1 umuC domain. SEQUENCE CAUTION: Sequence=AAK43708.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UBZ9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF151538 - Homo sapiens deoxycytidyl transferase (REV1) mRNA, complete cds. AB047646 - Homo sapiens mRNA for Rev1S, complete cds. AF206019 - Homo sapiens REV1 protein (REV1) mRNA, complete cds. BC037734 - Homo sapiens REV1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:26225 IMAGE:4824689), complete cds. AF357886 - Homo sapiens terminal deoxycytidyl transferase (REV1L) mRNA, complete cds. AK307719 - Homo sapiens cDNA, FLJ97667. AJ131720 - Homo sapiens mRNA for alpha integrin binding protein 80, partial. JD069029 - Sequence 50053 from Patent EP1572962. BC130411 - Homo sapiens REV1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:163283 IMAGE:40146442), complete cds. JD193722 - Sequence 174746 from Patent EP1572962. JD103729 - Sequence 84753 from Patent EP1572962. AK025176 - Homo sapiens cDNA: FLJ21523 fis, clone COL05890, highly similar to AF206019 Homo sapiens REV1 protein (REV1) mRNA. LF317448 - JP 2014500723-A/124951: Polycomb-Associated Non-Coding RNAs. MA553025 - JP 2018138019-A/124951: Polycomb-Associated Non-Coding RNAs. AK002087 - Homo sapiens cDNA FLJ11225 fis, clone PLACE1008275, weakly similar to DNA REPAIR PROTEIN REV1 (EC 2.7.7.-). JD141265 - Sequence 122289 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9UBZ9 (Reactome details) participates in the following event(s):
R-HSA-110307 REV1 binds AP-dsDNA R-HSA-5655835 POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template R-HSA-5656105 POLI simultaneously binds REV1 and monoUb:K164-PCNA at damaged DNA R-HSA-5655965 POLK and POLZ cooperate in elongation of mispaired primer termini R-HSA-5656158 POLZ elongates POLI-incorporated dNMP R-HSA-110308 REV1 inserts dCMP opposite to AP sites in DNA R-HSA-5652151 REV1 recruits POLZ to (AP:Cyt)-DNA Template R-HSA-110311 POLZ extends translesion synthesis R-HSA-5655892 POLK incorporates dNMP opposite to damaged DNA base R-HSA-5656148 POLI incorporates dNMP opposite to damaged DNA base R-HSA-5653770 USP10 deubiquitinates monoUb:K164,ISG:K164,ISG:K168-PCNA R-HSA-5653756 TRIM25 binds monoUb:164-PCNA R-HSA-5653766 USP10 binds monoUb:K164,ISG:K164,ISG:K168-PCNA R-HSA-5653754 UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA R-HSA-110312 Translesion synthesis by REV1 R-HSA-5655862 Translesion synthesis by POLK R-HSA-5656121 Translesion synthesis by POLI R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-73894 DNA Repair