Human Gene OSBPL6 (ENST00000357080.8) from GENCODE V44
  Description: Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-2372709, EBI-6248094; (from UniProt Q9BZF3)
RefSeq Summary (NM_001201482): This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000357080.8
Gencode Gene: ENSG00000079156.17
Transcript (Including UTRs)
   Position: hg38 chr2:178,194,500-178,382,779 Size: 188,280 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg38 chr2:178,306,185-178,382,614 Size: 76,430 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:178,194,500-178,382,779)mRNA (may differ from genome)Protein (508 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
LynxMalacardsMGIneXtProtPubMedReactome
UniProtKB

-  Comments and Description Text from UniProtKB
  ID: OSBL6_HUMAN
DESCRIPTION: RecName: Full=Oxysterol-binding protein-related protein 6; Short=ORP-6; Short=OSBP-related protein 6;
INTERACTION: Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-2372709, EBI-6248094;
TISSUE SPECIFICITY: Widely expressed.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the OSBP family.
SIMILARITY: Contains 1 PH domain.
SEQUENCE CAUTION: Sequence=BAB55223.1; Type=Erroneous initiation; Sequence=BAD92135.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 6.74 RPKM in Muscle - Skeletal
Total median expression: 78.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -172.20525-0.328 Picture PostScript Text
3' UTR -22.50165-0.136 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000648 - Oxysterol-bd
IPR018494 - Oxysterol-bd_CS
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology

Pfam Domains:
PF01237 - Oxysterol-binding protein

ModBase Predicted Comparative 3D Structure on Q9BZF3
FrontTopSide
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008289 lipid binding
GO:0015248 sterol transporter activity

Biological Process:
GO:0006699 bile acid biosynthetic process
GO:0006869 lipid transport
GO:0015918 sterol transport

Cellular Component:
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031965 nuclear membrane
GO:0097038 perinuclear endoplasmic reticulum


-  Descriptions from all associated GenBank mRNAs
  AB208898 - Homo sapiens mRNA for oxysterol-binding protein-like protein 6 isoform a variant protein.
BC052259 - Homo sapiens oxysterol binding protein-like 6, mRNA (cDNA clone MGC:59642 IMAGE:6428611), complete cds.
AK300389 - Homo sapiens cDNA FLJ60071 complete cds, highly similar to Oxysterol-binding protein-related protein 6.
AF323728 - Homo sapiens OSBP-related protein 6 mRNA, complete cds.
AF392448 - Homo sapiens oxysterol-binding protein-like protein OSBPL6 (OSBPL6) mRNA, complete cds.
AF462443 - Homo sapiens OSBP-related protein 6 isoform mRNA, complete cds.
AB384766 - Synthetic construct DNA, clone: pF1KB3241, Homo sapiens OSBPL6 gene for oxysterol-binding protein-related protein 6, complete cds, without stop codon, in Flexi system.
AK093902 - Homo sapiens cDNA FLJ36583 fis, clone TRACH2013123, moderately similar to Homo sapiens oxysterol binding protein-related protein 3 (ORP3) mRNA.
AX748360 - Sequence 1885 from Patent EP1308459.
AK027600 - Homo sapiens cDNA FLJ14694 fis, clone NT2RP2005407, weakly similar to OXYSTEROL-BINDING PROTEIN.
BC021248 - Homo sapiens oxysterol binding protein-like 6, mRNA (cDNA clone IMAGE:2822251), partial cds.
JD547077 - Sequence 528101 from Patent EP1572962.
JD106807 - Sequence 87831 from Patent EP1572962.
JD075139 - Sequence 56163 from Patent EP1572962.
JD350451 - Sequence 331475 from Patent EP1572962.
JD236235 - Sequence 217259 from Patent EP1572962.
CU690892 - Synthetic construct Homo sapiens gateway clone IMAGE:100022461 5' read OSBPL6 mRNA.
KJ903647 - Synthetic construct Homo sapiens clone ccsbBroadEn_13041 OSBPL6 gene, encodes complete protein.
BX647557 - Homo sapiens mRNA; cDNA DKFZp686M06110 (from clone DKFZp686M06110).

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BZF3 (Reactome details) participates in the following event(s):

R-HSA-8867667 OSBPs transport 25OH-CHOL from ER membrane to plasma membrane
R-HSA-192105 Synthesis of bile acids and bile salts
R-HSA-194068 Bile acid and bile salt metabolism
R-HSA-8957322 Metabolism of steroids
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B4DTW1, BC052259, C4AMC0, C4AME4, D3DPF6, D3DPF7, ENST00000357080.1, ENST00000357080.2, ENST00000357080.3, ENST00000357080.4, ENST00000357080.5, ENST00000357080.6, ENST00000357080.7, ORP6, OSBL6_HUMAN, Q4ZG68, Q53T68, Q59H61, Q7Z4Q1, Q8N9T0, Q96SR1, Q9BZF3, uc002ulw.1, uc002ulw.2, uc002ulw.3, uc002ulw.4
UCSC ID: ENST00000357080.8
RefSeq Accession: NM_001201482
Protein: Q9BZF3 (aka OSBL6_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.