Human Gene ZNF133 (ENST00000316358.8) from GENCODE V44
  Description: May be involved in transcriptional regulation as a repressor. (from UniProt P52736)
Gencode Transcript: ENST00000316358.8
Gencode Gene: ENSG00000125846.17
Transcript (Including UTRs)
   Position: hg38 chr20:18,297,985-18,316,996 Size: 19,012 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr20:18,305,687-18,316,816 Size: 11,130 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:18,297,985-18,316,996)mRNA (may differ from genome)Protein (654 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
LynxMalacardsMGIneXtProtPubMedReactome
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ZN133_HUMAN
DESCRIPTION: RecName: Full=Zinc finger protein 133; AltName: Full=Zinc finger protein 150;
FUNCTION: May be involved in transcriptional regulation as a repressor.
INTERACTION: P01100:FOS; NbExp=4; IntAct=EBI-2687350, EBI-852851;
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 15 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ZNF133
Diseases sorted by gene-association score: corneal dystrophy, posterior polymorphous, 1 (10), osteoblastoma (9), split hand-foot malformation 1 (9), bone benign neoplasm (6), connective tissue benign neoplasm (5), alagille syndrome 1 (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.68 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 256.25 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.9097-0.226 Picture PostScript Text
3' UTR -43.50180-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF01352 - KRAB box
PF00096 - Zinc finger, C2H2 type

ModBase Predicted Comparative 3D Structure on P52736
FrontTopSide
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0005515 protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  BC001887 - Homo sapiens zinc finger protein 133, mRNA (cDNA clone MGC:1579 IMAGE:3535533), complete cds.
AK295273 - Homo sapiens cDNA FLJ56079 complete cds, highly similar to Zinc finger protein 133.
AK295513 - Homo sapiens cDNA FLJ61274 complete cds, highly similar to Zinc finger protein 133.
AK291389 - Homo sapiens cDNA FLJ77414 complete cds, highly similar to Homo sapiens zinc finger protein 133 (clone pHZ-13) (ZNF133), mRNA.
AK316386 - Homo sapiens cDNA, FLJ79285 complete cds, highly similar to Zinc finger protein 133.
AK313525 - Homo sapiens cDNA, FLJ94083.
AK294263 - Homo sapiens cDNA FLJ61226 complete cds, highly similar to Zinc finger protein 133.
U09366 - Human zinc finger protein ZNF133.
JD349915 - Sequence 330939 from Patent EP1572962.
JD098653 - Sequence 79677 from Patent EP1572962.
AK074502 - Homo sapiens cDNA FLJ90021 fis, clone HEMBA1000875.
CU674910 - Synthetic construct Homo sapiens gateway clone IMAGE:100017758 5' read ZNF133 mRNA.
KJ897770 - Synthetic construct Homo sapiens clone ccsbBroadEn_07164 ZNF133 gene, encodes complete protein.
AB385083 - Synthetic construct DNA, clone: pF1KB5335, Homo sapiens ZNF133 gene for zinc finger protein 133, complete cds, without stop codon, in Flexi system.
BT007310 - Homo sapiens zinc finger protein 133 (clone pHZ-13) mRNA, complete cds.
AK123005 - Homo sapiens cDNA FLJ16801 fis, clone TESTI4004626, highly similar to Zinc finger protein 133.
DQ598046 - Homo sapiens piRNA piR-36112, complete sequence.
JD083969 - Sequence 64993 from Patent EP1572962.
JD419161 - Sequence 400185 from Patent EP1572962.
JD313001 - Sequence 294025 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P52736 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: A8K5S4, ENST00000316358.1, ENST00000316358.2, ENST00000316358.3, ENST00000316358.4, ENST00000316358.5, ENST00000316358.6, ENST00000316358.7, NM_001387298, P52736, Q53XU1, Q5JXV8, Q9BUV2, Q9H443, uc002wqm.1, uc002wqm.2, uc002wqm.3, ZN133_HUMAN, ZNF150
UCSC ID: ENST00000316358.8
RefSeq Accession: NM_001352463
Protein: P52736 (aka ZN133_HUMAN or Z133_HUMAN)
CCDS: CCDS13134.1, CCDS63234.1, CCDS63233.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.