Human Gene KREMEN1 (ENST00000400335.9) from GENCODE V44
  Description: Homo sapiens kringle containing transmembrane protein 1 (KREMEN1), transcript variant 3, mRNA. (from RefSeq NM_001039570)
RefSeq Summary (NM_001039570): This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000400335.9
Gencode Gene: ENSG00000183762.13
Transcript (Including UTRs)
   Position: hg38 chr22:29,073,035-29,146,820 Size: 73,786 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg38 chr22:29,073,131-29,142,112 Size: 68,982 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:29,073,035-29,146,820)mRNA (may differ from genome)Protein (458 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KREM1_HUMAN
DESCRIPTION: RecName: Full=Kremen protein 1; AltName: Full=Dickkopf receptor; AltName: Full=Kringle domain-containing transmembrane protein 1; AltName: Full=Kringle-containing protein marking the eye and the nose; Flags: Precursor;
FUNCTION: Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential).
SIMILARITY: Contains 1 CUB domain.
SIMILARITY: Contains 1 kringle domain.
SIMILARITY: Contains 1 WSC domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: KREMEN1
Diseases sorted by gene-association score: ectodermal dysplasia 13, hair/tooth type* (1230), osteoblastoma (8), hyperostosis (6), van buchem disease (6), bone benign neoplasm (5)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.69 RPKM in Esophagus - Mucosa
Total median expression: 282.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.9096-0.405 Picture PostScript Text
3' UTR -1933.004708-0.411 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000859 - CUB
IPR017076 - Kremen
IPR000001 - Kringle
IPR013806 - Kringle-like
IPR018056 - Kringle_CS
IPR002889 - WSC_carb-bd

Pfam Domains:
PF00431 - CUB domain
PF00051 - Kringle domain
PF01822 - WSC domain

ModBase Predicted Comparative 3D Structure on Q96MU8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006915 apoptotic process
GO:0007154 cell communication
GO:0016055 Wnt signaling pathway
GO:0030279 negative regulation of ossification
GO:0060173 limb development
GO:0060828 regulation of canonical Wnt signaling pathway
GO:0090090 negative regulation of canonical Wnt signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC063787 - Homo sapiens kringle containing transmembrane protein 1, mRNA (cDNA clone MGC:70445 IMAGE:6315614), complete cds.
AK056425 - Homo sapiens cDNA FLJ31863 fis, clone NT2RP7001591, highly similar to bK57G9.1 (novel Kringle and CUB domain protein).
AB059618 - Homo sapiens kremen mRNA for kringle-containing transmembrane protein, complete cds.
DQ582007 - Homo sapiens piRNA piR-32119, complete sequence.
JD386736 - Sequence 367760 from Patent EP1572962.
JD263830 - Sequence 244854 from Patent EP1572962.
JD437975 - Sequence 418999 from Patent EP1572962.
JD174356 - Sequence 155380 from Patent EP1572962.
JD534976 - Sequence 516000 from Patent EP1572962.
JD442124 - Sequence 423148 from Patent EP1572962.
JD394959 - Sequence 375983 from Patent EP1572962.
JD260769 - Sequence 241793 from Patent EP1572962.
JD220703 - Sequence 201727 from Patent EP1572962.
JD223342 - Sequence 204366 from Patent EP1572962.
JD543245 - Sequence 524269 from Patent EP1572962.
JD478554 - Sequence 459578 from Patent EP1572962.
JD463795 - Sequence 444819 from Patent EP1572962.
JD463796 - Sequence 444820 from Patent EP1572962.
JD148282 - Sequence 129306 from Patent EP1572962.
JD174087 - Sequence 155111 from Patent EP1572962.
JD265234 - Sequence 246258 from Patent EP1572962.
JD324647 - Sequence 305671 from Patent EP1572962.
JD148283 - Sequence 129307 from Patent EP1572962.
JD148284 - Sequence 129308 from Patent EP1572962.
JD249813 - Sequence 230837 from Patent EP1572962.
JD235378 - Sequence 216402 from Patent EP1572962.
JD296443 - Sequence 277467 from Patent EP1572962.
JD553177 - Sequence 534201 from Patent EP1572962.
JD193972 - Sequence 174996 from Patent EP1572962.
JD537068 - Sequence 518092 from Patent EP1572962.
JD491213 - Sequence 472237 from Patent EP1572962.
JD238076 - Sequence 219100 from Patent EP1572962.
JD217494 - Sequence 198518 from Patent EP1572962.
JD478278 - Sequence 459302 from Patent EP1572962.
JD375800 - Sequence 356824 from Patent EP1572962.
JD081397 - Sequence 62421 from Patent EP1572962.
JD222175 - Sequence 203199 from Patent EP1572962.
JD243298 - Sequence 224322 from Patent EP1572962.
JD243297 - Sequence 224321 from Patent EP1572962.
JD079666 - Sequence 60690 from Patent EP1572962.
JD079665 - Sequence 60689 from Patent EP1572962.
JD545843 - Sequence 526867 from Patent EP1572962.
JD487512 - Sequence 468536 from Patent EP1572962.
JD545842 - Sequence 526866 from Patent EP1572962.
JD171565 - Sequence 152589 from Patent EP1572962.
JD238468 - Sequence 219492 from Patent EP1572962.
JD047770 - Sequence 28794 from Patent EP1572962.
DQ597715 - Homo sapiens piRNA piR-35781, complete sequence.
AK172861 - Homo sapiens cDNA FLJ24091 fis, clone COL10314.
DQ574925 - Homo sapiens piRNA piR-43037, complete sequence.
DQ575410 - Homo sapiens piRNA piR-43522, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96MU8 (Reactome details) participates in the following event(s):

R-HSA-3769401 DKK and KRM bind LRP5/6
R-HSA-3772470 Negative regulation of TCF-dependent signaling by WNT ligand antagonists
R-HSA-5339717 Misspliced LRP5 mutants have enhanced beta-catenin-dependent signaling
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-4791275 Signaling by WNT in cancer
R-HSA-195721 Signaling by WNT
R-HSA-5663202 Diseases of signal transduction
R-HSA-162582 Signal Transduction
R-HSA-1643685 Disease

-  Other Names for This Gene
  Alternate Gene Symbols: B0QY46, B0QY47, B1AJR5, ENST00000400335.1, ENST00000400335.2, ENST00000400335.3, ENST00000400335.4, ENST00000400335.5, ENST00000400335.6, ENST00000400335.7, ENST00000400335.8, KREM1_HUMAN, KREMEN, KRM1, NM_001039570, Q5TIB9, Q6P3X6, Q96MU8, Q9BY70, Q9UGS5, Q9UGU1, uc003ael.1, uc003ael.2, uc003ael.3, uc003ael.4
UCSC ID: ENST00000400335.9
RefSeq Accession: NM_001039570
Protein: Q96MU8 (aka KREM1_HUMAN)
CCDS: CCDS43000.2

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.