Human Gene ZNF621 (ENST00000403205.6) from GENCODE V44
  Description: May be involved in transcriptional regulation. (from UniProt Q6ZSS3)
Gencode Transcript: ENST00000403205.6
Gencode Gene: ENSG00000172888.12
Transcript (Including UTRs)
   Position: hg38 chr3:40,524,878-40,533,526 Size: 8,649 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg38 chr3:40,525,841-40,533,090 Size: 7,250 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:40,524,878-40,533,526)mRNA (may differ from genome)Protein (439 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
HPRDLynxMalacardsMGIneXtProtPubMed
ReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ZN621_HUMAN
DESCRIPTION: RecName: Full=Zinc finger protein 621;
FUNCTION: May be involved in transcriptional regulation.
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 7 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.30 RPKM in Cells - Cultured fibroblasts
Total median expression: 163.50 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -91.60223-0.411 Picture PostScript Text
3' UTR -122.60436-0.281 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF01352 - KRAB box

ModBase Predicted Comparative 3D Structure on Q6ZSS3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  AK127181 - Homo sapiens cDNA FLJ45246 fis, clone BRCOC2012386, weakly similar to Zinc finger protein 184.
AX721066 - Sequence 26 from Patent WO0220754.
AK074366 - Homo sapiens cDNA FLJ23786 fis, clone HEP21350.
BC101619 - Homo sapiens zinc finger protein 621, mRNA (cDNA clone MGC:126668 IMAGE:8069125), complete cds.
BC113415 - Homo sapiens zinc finger protein 621, mRNA (cDNA clone MGC:141975 IMAGE:8322467), complete cds.
CR749268 - Homo sapiens mRNA; cDNA DKFZp686B11107 (from clone DKFZp686B11107).
JD043049 - Sequence 24073 from Patent EP1572962.
AB464409 - Synthetic construct DNA, clone: pF1KB7269, Homo sapiens ZNF621 gene for zinc finger protein 621, without stop codon, in Flexi system.
JD530303 - Sequence 511327 from Patent EP1572962.
EU446630 - Synthetic construct Homo sapiens clone IMAGE:100069876; IMAGE:100011839; FLH258540.01L zinc finger protein 621 (ZNF621) gene, encodes complete protein.
HQ258423 - Synthetic construct Homo sapiens clone IMAGE:100072852 zinc finger protein 621 (ZNF621), transcript variant 2 (ZNF621) gene, encodes complete protein.
KJ896000 - Synthetic construct Homo sapiens clone ccsbBroadEn_05394 ZNF621 gene, encodes complete protein.
AX721112 - Sequence 72 from Patent WO0220754.
JD316290 - Sequence 297314 from Patent EP1572962.
JD314885 - Sequence 295909 from Patent EP1572962.
JD414475 - Sequence 395499 from Patent EP1572962.
JD514431 - Sequence 495455 from Patent EP1572962.
JD371268 - Sequence 352292 from Patent EP1572962.
JD337563 - Sequence 318587 from Patent EP1572962.
JD463087 - Sequence 444111 from Patent EP1572962.
JD413729 - Sequence 394753 from Patent EP1572962.
JD155486 - Sequence 136510 from Patent EP1572962.
JD060220 - Sequence 41244 from Patent EP1572962.
JD533826 - Sequence 514850 from Patent EP1572962.
JD321846 - Sequence 302870 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q6ZSS3 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: AK127181, ENST00000403205.1, ENST00000403205.2, ENST00000403205.3, ENST00000403205.4, ENST00000403205.5, Q14DC7, Q6ZSS3, Q8TE91, uc003ckm.1, uc003ckm.2, uc003ckm.3, uc003ckm.4, ZN621_HUMAN
UCSC ID: ENST00000403205.6
RefSeq Accession: NM_001098414
Protein: Q6ZSS3 (aka ZN621_HUMAN)
CCDS: CCDS2693.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.