Human Gene CMBL (ENST00000296658.4) from GENCODE V44
Description: Homo sapiens carboxymethylenebutenolidase homolog (CMBL), mRNA. (from RefSeq NM_138809) RefSeq Summary (NM_138809): CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]. Gencode Transcript: ENST00000296658.4 Gencode Gene: ENSG00000164237.9 Transcript (Including UTRs) Position: hg38 chr5:10,277,595-10,307,902 Size: 30,308 Total Exon Count: 6 Strand: - Coding Region Position: hg38 chr5:10,280,453-10,290,762 Size: 10,310 Coding Exon Count: 5
ID:CMBL_HUMAN DESCRIPTION: RecName: Full=Carboxymethylenebutenolidase homolog; EC=3.1.-.-; FUNCTION: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. ENZYME REGULATION: Strongly inhibited by p-chloromercuribenzoate (PCMB). Partially inhibited by bis-p-nitrophenylphosphate (BNPP). Not inhibited by DFP, PMSF, eserine or EDTA. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=170 uM for olmesartan medoxomil; KM=283 uM for faropenem medoxomil; KM=63.4 uM for lenampicillin; Vmax=24.6 nmol/min/mg enzyme toward olmesartan medoxomil; Vmax=16.4 nmol/min/mg enzyme toward faropenem medoxomil; Vmax=4 nmol/min/mg enzyme toward lenampicillin; SUBCELLULAR LOCATION: Cytoplasm, cytosol. TISSUE SPECIFICITY: Widely expressed, with highest levels in liver, followed by kidney, small intestine and colon. Present in liver and intestine (at protein level). SIMILARITY: Belongs to the dienelactone hydrolase family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01738 - Dienelactone hydrolase family
ModBase Predicted Comparative 3D Structure on Q96DG6
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
