Human Gene MAT2B (ENST00000280969.9) from GENCODE V44
  Description: Homo sapiens methionine adenosyltransferase 2B (MAT2B), transcript variant 2, mRNA. (from RefSeq NM_182796)
RefSeq Summary (NM_182796): The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012].
Gencode Transcript: ENST00000280969.9
Gencode Gene: ENSG00000038274.18
Transcript (Including UTRs)
   Position: hg38 chr5:163,503,114-163,519,336 Size: 16,223 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr5:163,503,395-163,518,363 Size: 14,969 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:163,503,114-163,519,336)mRNA (may differ from genome)Protein (323 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MAT2B_HUMAN
DESCRIPTION: RecName: Full=Methionine adenosyltransferase 2 subunit beta; AltName: Full=Methionine adenosyltransferase II beta; Short=MAT II beta; AltName: Full=Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase;
FUNCTION: Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S- adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S- adenosylmethionine inhibition.
PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
SUBUNIT: Heterotetramer composed of 2 catalytic alpha subunits (alpha and alpha') (MAT2A) and 1 copy of beta subunit (MAT2B).
TISSUE SPECIFICITY: Widely expressed.
MISCELLANEOUS: Its expression in hepatoma cell lines may lead to increase DNA synthesis and thereby participate in cell proliferation.
SIMILARITY: Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.
SEQUENCE CAUTION: Sequence=AAG39296.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 31.63 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 830.05 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -78.20281-0.278 Picture PostScript Text
3' UTR -202.10973-0.208 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005913 - dTDP_dehydrorham_reduct
IPR016040 - NAD(P)-bd_dom

Pfam Domains:
PF04321 - RmlD substrate binding domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2YDX - X-ray MuPIT 2YDY - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NZL9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD Ensembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0019899 enzyme binding
GO:0048270 methionine adenosyltransferase regulator activity

Biological Process:
GO:0006556 S-adenosylmethionine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0050790 regulation of catalytic activity

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005829 cytosol
GO:0048269 methionine adenosyltransferase complex
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF209036 - JP 2014500723-A/16539: Polycomb-Associated Non-Coding RNAs.
MA444613 - JP 2018138019-A/16539: Polycomb-Associated Non-Coding RNAs.
DQ395260 - Homo sapiens methionine adenosyltransferase beta subunit variant II (MAT2B) mRNA, complete cds, alternatively spliced.
DQ413183 - Homo sapiens methionine adenosyltransferase II beta variant 2 (MAT2B) mRNA, complete cds, alternatively spliced.
AJ243721 - Homo sapiens mRNA for dTDP-4-keto-6-deoxy-D-glucose 4-reductase (tgr gene).
AK291919 - Homo sapiens cDNA FLJ76904 complete cds, highly similar to Homo sapiens methionine adenosyltransferase II, beta (MAT2B), transcript variant 2, mRNA.
BC005218 - Homo sapiens methionine adenosyltransferase II, beta, mRNA (cDNA clone MGC:12237 IMAGE:3931953), complete cds.
AY358695 - Homo sapiens clone DNA66307 MAT2B (UNQ2435) mRNA, complete cds.
KJ906126 - Synthetic construct Homo sapiens clone ccsbBroadEn_15796 MAT2B gene, encodes complete protein.
AK312365 - Homo sapiens cDNA, FLJ92687, Homo sapiens methionine adenosyltransferase II, beta (MAT2B), mRNA.
AB073390 - Homo sapiens primary neuroblastoma cDNA, clone:Nbla02999, full insert sequence.
BC093030 - Homo sapiens methionine adenosyltransferase II, beta, mRNA (cDNA clone MGC:110930 IMAGE:30527606), complete cds.
AL136664 - Homo sapiens mRNA; cDNA DKFZp564B246 (from clone DKFZp564B246).
AK096178 - Homo sapiens cDNA FLJ38859 fis, clone MESAN2011632, highly similar to Homo sapiens mRNA for dTDP-4-keto-6-deoxy-D-glucose 4-reductase (tgr gene).
HM005431 - Homo sapiens clone HTL-T-118 testicular tissue protein Li 118 mRNA, complete cds.
BC066645 - Homo sapiens methionine adenosyltransferase II, beta, mRNA (cDNA clone MGC:70595 IMAGE:5749043), complete cds.
CU688168 - Synthetic construct Homo sapiens gateway clone IMAGE:100021695 5' read MAT2B mRNA.
KJ898695 - Synthetic construct Homo sapiens clone ccsbBroadEn_08089 MAT2B gene, encodes complete protein.
AB528179 - Synthetic construct DNA, clone: pF1KE0413, Homo sapiens MAT2B gene for methionine adenosyltransferase II, beta, without stop codon, in Flexi system.
AM393097 - Synthetic construct Homo sapiens clone IMAGE:100001853 for hypothetical protein (MAT2B gene).
AM393737 - Synthetic construct Homo sapiens clone IMAGE:100001860 for hypothetical protein (MAT2B gene).
AF182814 - Homo sapiens methionine adenosyltransferase regulatory beta subunit mRNA, complete cds.
AF113225 - Homo sapiens MSTP045 mRNA, complete cds.
AK294069 - Homo sapiens cDNA FLJ55836 complete cds, highly similar to Homo sapiens methionine adenosyltransferase II, beta (MAT2B), transcript variant 2, mRNA.
LF325419 - JP 2014500723-A/132922: Polycomb-Associated Non-Coding RNAs.
MA560996 - JP 2018138019-A/132922: Polycomb-Associated Non-Coding RNAs.
LF325417 - JP 2014500723-A/132920: Polycomb-Associated Non-Coding RNAs.
MA560994 - JP 2018138019-A/132920: Polycomb-Associated Non-Coding RNAs.
DQ413182 - Homo sapiens nonfunctional methionine adenosyltransferase II beta variant 3 (MAT2B) mRNA, complete sequence, alternatively spliced.
JD533588 - Sequence 514612 from Patent EP1572962.
JD077835 - Sequence 58859 from Patent EP1572962.
JD341242 - Sequence 322266 from Patent EP1572962.
JD561945 - Sequence 542969 from Patent EP1572962.
JD500087 - Sequence 481111 from Patent EP1572962.
JD417264 - Sequence 398288 from Patent EP1572962.
JD326667 - Sequence 307691 from Patent EP1572962.
JD083022 - Sequence 64046 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00270 - Cysteine and methionine metabolism
hsa00450 - Selenoamino acid metabolism
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q9NZL9 (Reactome details) participates in the following event(s):

R-HSA-5603114 MAT2B:MAT2A:K+:2Mg2+ transfers Ado from ATP to L-Met
R-HSA-5696872 USP30 deubiquitinates Ub-MOM proteins
R-HSA-156581 Methylation
R-HSA-5689880 Ub-specific processing proteases
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-5688426 Deubiquitination
R-HSA-211859 Biological oxidations
R-HSA-597592 Post-translational protein modification
R-HSA-1430728 Metabolism
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B2R5Y6, ENST00000280969.1, ENST00000280969.2, ENST00000280969.3, ENST00000280969.4, ENST00000280969.5, ENST00000280969.6, ENST00000280969.7, ENST00000280969.8, MAT2B_HUMAN, MSTP045, Nbla02999, NM_182796, Q1WAI7, Q27J92, Q3LIE8, Q567T7, Q6NYC7, Q9BS89, Q9H3E1, Q9NZL9, Q9UJ54, TGR, uc003lzj.1, uc003lzj.2, uc003lzj.3, uc003lzj.4, uc003lzj.5, UNQ2435/PRO4995
UCSC ID: ENST00000280969.9
RefSeq Accession: NM_182796
Protein: Q9NZL9 (aka MAT2B_HUMAN)
CCDS: CCDS4364.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.